基于小孔径支架和边缘密封水凝胶的再生丝素修复骨软骨缺损。

IF 11.3 1区 医学 Q1 Medicine Biomaterials Research Pub Date : 2023-05-19 DOI:10.1186/s40824-023-00370-1
Yinyue Luo, Menglin Xiao, Bushra Sufyan Almaqrami, Hong Kang, Zhengzhong Shao, Xin Chen, Ying Zhang
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引用次数: 2

摘要

背景:骨软骨缺损是一个巨大的挑战,至今没有令人满意的修复策略。特别是,新软骨与周围原生软骨的横向整合是决定组织修复成功的一个困难和不充分解决的问题。方法:采用正丁醇创新制备基于小孔径支架的再生丝素(RSF)。然后,在RSF支架上培养兔膝关节软骨细胞和骨间充质干细胞(BMSCs),诱导成软骨分化后,制备经14wt % RSF溶液强化的细胞-支架复合物进行体内实验。结果:制备并证实了具有生物相容性和良好粘附性能的多孔支架和RSF密封剂可促进软骨细胞迁移和分化。因此,这种复合材料在体内可实现骨软骨修复和良好的水平整合。结论:总的来说,RSF支架周围边缘封闭的新方法显示出卓越的修复效果,证实了这种新型移植物促进软骨-软骨下骨同时再生的能力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Regenerated silk fibroin based on small aperture scaffolds and marginal sealing hydrogel for osteochondral defect repair.

Background: Osteochondral defects pose an enormous challenge without satisfactory repair strategy to date. In particular, the lateral integration of neo-cartilage into the surrounding native cartilage is a difficult and inadequately addressed problem determining tissue repair's success.

Methods: Regenerated silk fibroin (RSF) based on small aperture scaffolds was prepared with n-butanol innovatively. Then, the rabbit knee chondrocytes and bone mesenchymal stem cells (BMSCs) were cultured on RSF scaffolds, and after induction of chondrogenic differentiation, cell-scaffold complexes strengthened by a 14 wt% RSF solution were prepared for in vivo experiments.

Results: A porous scaffold and an RSF sealant exhibiting biocompatibility and excellent adhesive properties are developed and confirmed to promote chondrocyte migration and differentiation. Thus, osteochondral repair and superior horizontal integration are achieved in vivo with this composite.

Conclusions: Overall, the new approach of marginal sealing around the RSF scaffolds exhibits preeminent repair results, confirming the ability of this novel graft to facilitate simultaneous regeneration of cartilage-subchondral bone.

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来源期刊
Biomaterials Research
Biomaterials Research Medicine-Medicine (miscellaneous)
CiteScore
10.20
自引率
3.50%
发文量
63
审稿时长
30 days
期刊介绍: Biomaterials Research, the official journal of the Korean Society for Biomaterials, is an open-access interdisciplinary publication that focuses on all aspects of biomaterials research. The journal covers a wide range of topics including novel biomaterials, advanced techniques for biomaterial synthesis and fabrication, and their application in biomedical fields. Specific areas of interest include functional biomaterials, drug and gene delivery systems, tissue engineering, nanomedicine, nano/micro-biotechnology, bio-imaging, regenerative medicine, medical devices, 3D printing, and stem cell research. By exploring these research areas, Biomaterials Research aims to provide valuable insights and promote advancements in the biomaterials field.
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