放射治疗对根内牙本质形态和组成结构的影响。

Q2 Medicine Brazilian dental journal Pub Date : 2023-01-01 DOI:10.1590/0103-6440202305101
Georgia Ribeiro Martini, Eduardo A Bortoluzzi, Mariana C Minamisako, Natalia C Trentin Bordignon, Paulo M Rodrigues, Rogério Gondak
{"title":"放射治疗对根内牙本质形态和组成结构的影响。","authors":"Georgia Ribeiro Martini,&nbsp;Eduardo A Bortoluzzi,&nbsp;Mariana C Minamisako,&nbsp;Natalia C Trentin Bordignon,&nbsp;Paulo M Rodrigues,&nbsp;Rogério Gondak","doi":"10.1590/0103-6440202305101","DOIUrl":null,"url":null,"abstract":"<p><p>Considering the side effects in the oral cavity and dental structures of radiotherapy (RDT) for head and neck cancer, this study aimed to evaluate the effects of RDT on the root dentin concerning the obliteration of dentinal tubules, the inorganic composition of intra-radicular dentin, and the integrity of collagen fibers. Thirty human canines were selected from a biobank and randomly divided into two groups (n=15). The samples were sectioned buccolingually, and a hemisection was used for structural analysis by scanning electron microscopy (SEM) and energy-dispersive X-ray spectrometer (EDS). Low-vacuum SEM images were obtained at 2000-x magnification to observe the obliteration of the dentinal tubules. Moreover, compositional evaluation was performed using EDS. After RDT, the SEM and EDS analyses were repeated using the same methodology. RDT was applied fractionally at 2 Gy per day, 5 days per week, for 7 weeks, resulting in a total dose of 70 Gy. The collagen integrity of the irradiated and non-irradiated samples was analyzed using Masson's trichrome and picrosirius red staining polarization microscopy. Samples subjected to RDT exhibited dentinal tubule obliteration (p < 0.001); low integrity of type I and III collagen fibers (p < 0.05); compositional reduction of calcium (p = 0.012), phosphorus (p = 0.001), and magnesium (p < 0.001); an increased Ca/P ratio (p < 0.001). RDT affects the structure of dentinal tubules, the inorganic composition of intra-radicular dentin, and the collagen fiber integrity in the root dentin, which may interfere with the effectiveness and durability of dental procedures.</p>","PeriodicalId":9211,"journal":{"name":"Brazilian dental journal","volume":"34 1","pages":"45-51"},"PeriodicalIF":0.0000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10027097/pdf/","citationCount":"1","resultStr":"{\"title\":\"Impact of radiotherapy on the morphological and compositional structure of intra-radicular dentin.\",\"authors\":\"Georgia Ribeiro Martini,&nbsp;Eduardo A Bortoluzzi,&nbsp;Mariana C Minamisako,&nbsp;Natalia C Trentin Bordignon,&nbsp;Paulo M Rodrigues,&nbsp;Rogério Gondak\",\"doi\":\"10.1590/0103-6440202305101\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Considering the side effects in the oral cavity and dental structures of radiotherapy (RDT) for head and neck cancer, this study aimed to evaluate the effects of RDT on the root dentin concerning the obliteration of dentinal tubules, the inorganic composition of intra-radicular dentin, and the integrity of collagen fibers. Thirty human canines were selected from a biobank and randomly divided into two groups (n=15). The samples were sectioned buccolingually, and a hemisection was used for structural analysis by scanning electron microscopy (SEM) and energy-dispersive X-ray spectrometer (EDS). Low-vacuum SEM images were obtained at 2000-x magnification to observe the obliteration of the dentinal tubules. Moreover, compositional evaluation was performed using EDS. After RDT, the SEM and EDS analyses were repeated using the same methodology. RDT was applied fractionally at 2 Gy per day, 5 days per week, for 7 weeks, resulting in a total dose of 70 Gy. The collagen integrity of the irradiated and non-irradiated samples was analyzed using Masson's trichrome and picrosirius red staining polarization microscopy. Samples subjected to RDT exhibited dentinal tubule obliteration (p < 0.001); low integrity of type I and III collagen fibers (p < 0.05); compositional reduction of calcium (p = 0.012), phosphorus (p = 0.001), and magnesium (p < 0.001); an increased Ca/P ratio (p < 0.001). RDT affects the structure of dentinal tubules, the inorganic composition of intra-radicular dentin, and the collagen fiber integrity in the root dentin, which may interfere with the effectiveness and durability of dental procedures.</p>\",\"PeriodicalId\":9211,\"journal\":{\"name\":\"Brazilian dental journal\",\"volume\":\"34 1\",\"pages\":\"45-51\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10027097/pdf/\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Brazilian dental journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1590/0103-6440202305101\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Brazilian dental journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1590/0103-6440202305101","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Medicine","Score":null,"Total":0}
引用次数: 1

摘要

考虑到头颈癌放射治疗对口腔及口腔结构的副作用,本研究旨在评价放射治疗对牙根本质的影响,包括牙本质小管的堵塞、根内牙本质的无机组成以及胶原纤维的完整性。从生物库中选取30只人犬,随机分为两组(n=15)。对样品进行斜口切片,用扫描电子显微镜(SEM)和能量色散x射线光谱仪(EDS)对样品进行半切片结构分析。在2000倍放大下获得低真空扫描电镜图像,观察牙本质小管的闭塞。并用能谱仪进行成分评价。RDT后,使用相同的方法重复扫描电镜和能谱分析。RDT以每天2 Gy的剂量,每周5天,连续7周,总剂量为70 Gy。用马松三色和小天狼红染色偏振显微镜分析辐照和未辐照样品的胶原完整性。接受RDT治疗的样品表现为牙本质小管闭塞(p < 0.001);I型和III型胶原纤维完整性低(p < 0.05);钙(p = 0.012)、磷(p = 0.001)和镁(p < 0.001)的成分减少;Ca/P比值升高(P < 0.001)。RDT会影响牙本质小管的结构、牙根内牙本质的无机成分和牙根内牙本质胶原纤维的完整性,从而影响牙科手术的有效性和持久性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

摘要图片

摘要图片

摘要图片

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Impact of radiotherapy on the morphological and compositional structure of intra-radicular dentin.

Considering the side effects in the oral cavity and dental structures of radiotherapy (RDT) for head and neck cancer, this study aimed to evaluate the effects of RDT on the root dentin concerning the obliteration of dentinal tubules, the inorganic composition of intra-radicular dentin, and the integrity of collagen fibers. Thirty human canines were selected from a biobank and randomly divided into two groups (n=15). The samples were sectioned buccolingually, and a hemisection was used for structural analysis by scanning electron microscopy (SEM) and energy-dispersive X-ray spectrometer (EDS). Low-vacuum SEM images were obtained at 2000-x magnification to observe the obliteration of the dentinal tubules. Moreover, compositional evaluation was performed using EDS. After RDT, the SEM and EDS analyses were repeated using the same methodology. RDT was applied fractionally at 2 Gy per day, 5 days per week, for 7 weeks, resulting in a total dose of 70 Gy. The collagen integrity of the irradiated and non-irradiated samples was analyzed using Masson's trichrome and picrosirius red staining polarization microscopy. Samples subjected to RDT exhibited dentinal tubule obliteration (p < 0.001); low integrity of type I and III collagen fibers (p < 0.05); compositional reduction of calcium (p = 0.012), phosphorus (p = 0.001), and magnesium (p < 0.001); an increased Ca/P ratio (p < 0.001). RDT affects the structure of dentinal tubules, the inorganic composition of intra-radicular dentin, and the collagen fiber integrity in the root dentin, which may interfere with the effectiveness and durability of dental procedures.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Brazilian dental journal
Brazilian dental journal Dentistry-Dentistry (all)
CiteScore
2.20
自引率
0.00%
发文量
69
审稿时长
12 weeks
期刊介绍: Brazilian Dental Journal, publishes Full-Length Papers, Short Communications and Case Reports, dealing with dentistry or related disciplines and edited six times a year.
期刊最新文献
Can a dentin bonding agent prevent color change in regenerative endodontic procedures? An in vitro evaluation Antibacterial ability of different activated irrigation after root canal preparation: intratubular analyses Inhibition of incipient caries lesion progression by different fluoridated varnishes A new hydroxyapatite-alginate-gelatin biocomposite favor bone regeneration in a critical-sized calvarial defect model Prevalence of lectures about dental esthetics and female speakers in three Brazilian conferences.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1