{"title":"三种临床治疗方法对大鼠股骨头早期骨坏死髋关节软骨细胞因子调节及骨改善的影响","authors":"Shan-Ling Hsu , Shun-Wun Jhan , Chieh-Cheng Hsu , Yi-No Wu , Kay L.H. Wu , Chun-En Aurea Kuo , Hung-Wen Chiu , Jai-Hong Cheng","doi":"10.1016/j.bj.2022.11.004","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>Extracorporeal shockwave therapy (ESWT) and adipose-derived mesenchymal stem cells (ADSCs) have been used clinically for the treatment of osteonecrosis of the femoral head (ONFH). The study elucidated that ESWT, ADSCs, and combination therapy modulated pro-inflammatory cytokines in the articular cartilage and subchondral bone of early rat ONFH.</p></div><div><h3>Methods</h3><p>ESWT and ADSCs were prepared and isolated for treatment. Micro-CT, pathological analysis, and immunohistochemistry were performed and analysed.</p></div><div><h3>Results</h3><p>After treatments, subchondral bone of ONFH was improved in trabecular bone volume (BV/TV) (<em>p</em> < 0.001), thickness (Tb.Th) (<em>p</em> < 0.01 and 0.001), and separation (Tb.Sp) (<em>p</em> < 0.001) and bone mineral density (BMD) (<em>p</em> < 0.001) using micro-CT analysis. The articular cartilage was protected and decreased apoptosis markers after all the treatments. The expression of IL33 (<em>p</em> < 0.001), IL5 (<em>p</em> < 0.001), IL6 (<em>p</em> < 0.001), and IL17A (<em>p</em> < 0.01) was significantly decreased in the ESWT, ADSCs, and Combination groups as compared with ONFH group. The IL33 receptor ST2 was significantly increased after treatment (<em>p</em> < 0.001) as compared with ONFH group. The Combination group (<em>p</em> < 0.01) decreased the expression of IL6 better than the ESWT and ADSCs groups.</p></div><div><h3>Conclusion</h3><p>ESWT, ADSCs and combination therapy significantly protected articular cartilage and subchondral bone of early rat ONFH by modulating the expression of pro-inflammatory cytokines including, IL33 and its receptor ST2, IL5, IL6, and IL17A.</p></div>","PeriodicalId":8934,"journal":{"name":"Biomedical Journal","volume":"46 6","pages":"Article 100571"},"PeriodicalIF":4.1000,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2319417022001561/pdfft?md5=c42212c1d0fb3ae32c011aaba963ee19&pid=1-s2.0-S2319417022001561-main.pdf","citationCount":"1","resultStr":"{\"title\":\"Effect of three clinical therapies on cytokines modulation in the hip articular cartilage and bone improvement in rat early osteonecrosis of the femoral head\",\"authors\":\"Shan-Ling Hsu , Shun-Wun Jhan , Chieh-Cheng Hsu , Yi-No Wu , Kay L.H. Wu , Chun-En Aurea Kuo , Hung-Wen Chiu , Jai-Hong Cheng\",\"doi\":\"10.1016/j.bj.2022.11.004\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>Extracorporeal shockwave therapy (ESWT) and adipose-derived mesenchymal stem cells (ADSCs) have been used clinically for the treatment of osteonecrosis of the femoral head (ONFH). The study elucidated that ESWT, ADSCs, and combination therapy modulated pro-inflammatory cytokines in the articular cartilage and subchondral bone of early rat ONFH.</p></div><div><h3>Methods</h3><p>ESWT and ADSCs were prepared and isolated for treatment. Micro-CT, pathological analysis, and immunohistochemistry were performed and analysed.</p></div><div><h3>Results</h3><p>After treatments, subchondral bone of ONFH was improved in trabecular bone volume (BV/TV) (<em>p</em> < 0.001), thickness (Tb.Th) (<em>p</em> < 0.01 and 0.001), and separation (Tb.Sp) (<em>p</em> < 0.001) and bone mineral density (BMD) (<em>p</em> < 0.001) using micro-CT analysis. The articular cartilage was protected and decreased apoptosis markers after all the treatments. The expression of IL33 (<em>p</em> < 0.001), IL5 (<em>p</em> < 0.001), IL6 (<em>p</em> < 0.001), and IL17A (<em>p</em> < 0.01) was significantly decreased in the ESWT, ADSCs, and Combination groups as compared with ONFH group. The IL33 receptor ST2 was significantly increased after treatment (<em>p</em> < 0.001) as compared with ONFH group. The Combination group (<em>p</em> < 0.01) decreased the expression of IL6 better than the ESWT and ADSCs groups.</p></div><div><h3>Conclusion</h3><p>ESWT, ADSCs and combination therapy significantly protected articular cartilage and subchondral bone of early rat ONFH by modulating the expression of pro-inflammatory cytokines including, IL33 and its receptor ST2, IL5, IL6, and IL17A.</p></div>\",\"PeriodicalId\":8934,\"journal\":{\"name\":\"Biomedical Journal\",\"volume\":\"46 6\",\"pages\":\"Article 100571\"},\"PeriodicalIF\":4.1000,\"publicationDate\":\"2023-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2319417022001561/pdfft?md5=c42212c1d0fb3ae32c011aaba963ee19&pid=1-s2.0-S2319417022001561-main.pdf\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biomedical Journal\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2319417022001561\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomedical Journal","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2319417022001561","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Effect of three clinical therapies on cytokines modulation in the hip articular cartilage and bone improvement in rat early osteonecrosis of the femoral head
Background
Extracorporeal shockwave therapy (ESWT) and adipose-derived mesenchymal stem cells (ADSCs) have been used clinically for the treatment of osteonecrosis of the femoral head (ONFH). The study elucidated that ESWT, ADSCs, and combination therapy modulated pro-inflammatory cytokines in the articular cartilage and subchondral bone of early rat ONFH.
Methods
ESWT and ADSCs were prepared and isolated for treatment. Micro-CT, pathological analysis, and immunohistochemistry were performed and analysed.
Results
After treatments, subchondral bone of ONFH was improved in trabecular bone volume (BV/TV) (p < 0.001), thickness (Tb.Th) (p < 0.01 and 0.001), and separation (Tb.Sp) (p < 0.001) and bone mineral density (BMD) (p < 0.001) using micro-CT analysis. The articular cartilage was protected and decreased apoptosis markers after all the treatments. The expression of IL33 (p < 0.001), IL5 (p < 0.001), IL6 (p < 0.001), and IL17A (p < 0.01) was significantly decreased in the ESWT, ADSCs, and Combination groups as compared with ONFH group. The IL33 receptor ST2 was significantly increased after treatment (p < 0.001) as compared with ONFH group. The Combination group (p < 0.01) decreased the expression of IL6 better than the ESWT and ADSCs groups.
Conclusion
ESWT, ADSCs and combination therapy significantly protected articular cartilage and subchondral bone of early rat ONFH by modulating the expression of pro-inflammatory cytokines including, IL33 and its receptor ST2, IL5, IL6, and IL17A.
期刊介绍：
Biomedical Journal publishes 6 peer-reviewed issues per year in all fields of clinical and biomedical sciences for an internationally diverse authorship. Unlike most open access journals, which are free to readers but not authors, Biomedical Journal does not charge for subscription, submission, processing or publication of manuscripts, nor for color reproduction of photographs.
Clinical studies, accounts of clinical trials, biomarker studies, and characterization of human pathogens are within the scope of the journal, as well as basic studies in model species such as Escherichia coli, Caenorhabditis elegans, Drosophila melanogaster, and Mus musculus revealing the function of molecules, cells, and tissues relevant for human health. However, articles on other species can be published if they contribute to our understanding of basic mechanisms of biology.
A highly-cited international editorial board assures timely publication of manuscripts. Reviews on recent progress in biomedical sciences are commissioned by the editors.
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