城市空气颗粒物修饰卵清蛋白免疫原性的研究

IF 2.9 4区 医学 Q3 IMMUNOLOGY Archivum Immunologiae et Therapiae Experimentalis Pub Date : 2023-05-28 DOI:10.1007/s00005-023-00679-8
Bernadeta Nowak, Anna Wądołek, Olga Mazuryk, Anna Poznańska, Katarzyna Majzner, Grzegorz Majka, Maria Oszajca, Małgorzata Barańska, Grażyna Stochel, Janusz Marcinkiewicz
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引用次数: 0

摘要

暴露于空气颗粒物(PM)与血液氧化应激和全身炎症有关。本研究的目的是阐明氧化PM对主要抗氧化血清蛋白卵清蛋白(OVA)的修饰是否会改变其抗原性和/或免疫原性。卵清蛋白通过透析暴露于标准城市PM (SRM 1648a)或去除有机含量的PM(编码为LAP)。测定了pm修饰OVA的结构变化和生物学特性。采用从C57BL/6和OT-II(323-339表位)OVA特异性T细胞受体(TCR)转基因小鼠中分离的T淋巴细胞和树突状细胞(主要抗原呈递细胞)检测PM对OVA免疫原性的影响。通过刺激细胞的表位特异性T细胞增殖和干扰素γ的产生,SRM 1648a和lap修饰的OVA的免疫原性均显著高于对照OVA。这种作用与OVA表位结构外的载体分子的轻度氧化变化以及pm修饰的OVA对蛋白水解的抵抗力增加有关。有趣的是,当树突状细胞用pm修饰的OVA培养时,树突状细胞显示出增强的蛋白质摄取能力。我们的研究结果表明,pm修饰的OVA的免疫原性增强与抗原性或抗原呈递的改变无关。然而,这可能是由于修饰抗原在树突状细胞中降解较慢和持续时间较长。这一现象是否与在城市PM高污染地区观察到的自身免疫性疾病风险患病率增加有关,需要解释。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Investigation of the Immunogenic Properties of Ovalbumin Modified by Urban Airborne Particulate Matter

Exposure to air particulate matter (PM) is linked to the blood oxidative stress and systemic inflammation. The aim of this study was to elucidate whether oxidative PM modification of ovalbumin (OVA), the major antioxidant serum protein, may alter its antigenicity and/or immunogenicity. Ovalbumin was exposed via dialysis to the standard urban PM (SRM 1648a) or to PM with removed organic content (encoded as LAP). Both structural changes and biological properties of PM-modified OVA were measured. T lymphocytes and dendritic cells (the major antigen-presenting cells) isolated from C57BL/6 and OT-II (323–339 epitope) OVA-specific T cell receptor (TCR)-transgenic mice were used to test the effect of PM on OVA immunogenicity. The immunogenicity of both SRM 1648a and LAP-modified OVA was significantly higher than that of control OVA, as measured by the epitope-specific T cell proliferation and interferon γ production by the stimulated cells. This effect was associated with mild oxidative changes in the carrier molecule outside the structure of the OVA epitope and with increased resistance to proteolysis of PM-modified OVA. Interestingly, dendritic cells showed enhanced capacity for the uptake of proteins when the cells were cultured with PM-modified OVA. Our results suggest that the enhanced immunogenicity of PM-modified OVA is not associated with altered antigenicity or antigen presentation. However, it may result from slower degradation and longer persistence of modified antigens in dendritic cells. Whether this phenomenon is associated with enhanced risk prevalence of autoimmune diseases observed in the areas with high urban PM pollution needs to be explained.

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来源期刊
CiteScore
5.90
自引率
0.00%
发文量
26
审稿时长
>12 weeks
期刊介绍: Archivum Immunologiae et Therapiae Experimentalis (AITE), founded in 1953 by Ludwik Hirszfeld, is a bimonthly, multidisciplinary journal. It publishes reviews and full original papers dealing with immunology, experimental therapy, immunogenetics, transplantation, microbiology, immunochemistry and ethics in science.
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