评估三丁基氯化锡对成年白化大鼠垂体-睾丸轴诱导的组织形态学和生化变化以及橙皮甙可能发挥的改善作用

IF 1.1 4区 医学 Q4 MICROSCOPY Ultrastructural Pathology Pub Date : 2023-07-04 Epub Date: 2023-03-29 DOI:10.1080/01913123.2023.2195489
Sahar F Shaban, Maha A Khattab, Samar H Abd El Hameed, Shaimaa A Abdelrahman
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引用次数: 0

摘要

本研究旨在详细探讨三丁基氯化锡(TBT)对垂体-睾丸轴的毒性作用,以及橙皮甙对其可能的改善作用。72 只成年雄性白化大鼠被分为四组:对照组(I)、TBT 处理组(II)、TBT+橙皮甙组(III)和恢复组(IV)。测量体重和睾丸重量。采集血样,通过酶联免疫吸附试验(ELISA)评估血清中睾酮、FSH 和 LH 激素的水平。测量睾丸匀浆中丙二醛(MDA)的水平。对垂体和睾丸组织样本进行光镜和电子显微镜检查,并对抗 FSH 和 Ki67 蛋白进行免疫组化检测。结果显示,与对照组相比,三丁基锡化合物组的睾丸重量、血清睾酮、前列腺素和促甲状腺激素水平明显下降,而组织中的 MDA 则明显增加。三丁基锡化合物治疗引起了严重的组织病理学变化,垂体中 PAS 染色的嗜碱性粒细胞和抗 FSH 免疫染色的促性腺激素的面积百分比下降。第二组的睾丸也出现了明显的组织损伤、细胞脱落,上皮高度下降,增殖的生精细胞数量减少。事实证明,用 TBT 补充橙皮甙可显著改善两个腺体的上述参数,从而提高男性生育能力。总之:黄酮类化合物橙皮甙有可能保护易感人群免受三丁基锡化合物对生殖系统的损害。
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Evaluating the histomorphological and biochemical changes induced by Tributyltin Chloride on pituitary-testicular axis of adult albino rats and the possible ameliorative role of hesperidin.

This study was performed to explore in detail the toxic effects of Tributyltin Chloride (TBT) on the pituitary-testicular axis and the possible amelioration with Hesperidin. Seventy-two adult male albino rats were divided into four groups: Control group (I), TBT-treated group (II), TBT+Hesperidin group (III), and Recovery group (IV). Body and testicular weights were measured. Blood samples were taken to estimate serum levels of testosterone, FSH and LH hormones by enzyme-linked immunosorbent assay (ELISA). Malondialdehyde (MDA) level was measured in testes homogenates. Tissue samples from the pituitary glands and testes were processed for light, electron microscope examination, and immunohistochemical detection of anti-FSH, and Ki67 proteins. Results showed a statistically significant decrease in testicular weight, serum testosterone, FSH and LH levels and a significant increase in tissue MDA in the TBT group when compared to the control group. TBT treatment caused severe histopathological changes with decreased area percent of PAS-stained basophils, and anti FSH immuno-stained gonadotrophs in the pituitary gland. The testes of group II also showed marked tissue damage, cell loss with decreased epithelial height and decreased number of proliferating spermatogenic cells. Hesperidin supplementation with TBT proved significant amelioration of the previously mentioned parameters in both glands which could improve male fertility. In conclusion: The flavonoid Hesperidin has the potential to protect against the reproductive damage induced by TBT in susceptible individuals.

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来源期刊
Ultrastructural Pathology
Ultrastructural Pathology 医学-病理学
CiteScore
2.00
自引率
10.00%
发文量
40
审稿时长
6-12 weeks
期刊介绍: Ultrastructural Pathology is the official journal of the Society for Ultrastructural Pathology. Published bimonthly, we are the only journal to be devoted entirely to diagnostic ultrastructural pathology. Ultrastructural Pathology is the ideal journal to publish high-quality research on the following topics: Advances in the uses of electron microscopic and immunohistochemical techniques Correlations of ultrastructural data with light microscopy, histochemistry, immunohistochemistry, biochemistry, cell and tissue culturing, and electron probe analysis Important new, investigative, clinical, and diagnostic EM methods.
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