{"title":"C-ACT激活凝血时间测试的参考间隔,在健康的狗使用水浴和人工评估凝血形成。","authors":"K J Nash, S K Day, W A Goodwin","doi":"10.1080/00480169.2023.2201222","DOIUrl":null,"url":null,"abstract":"<p><strong>Aims: </strong>To establish a reference range for the canine C-ACT activated clotting time (ACT) test using a water bath and visual clot assessment technique.</p><p><strong>Methods: </strong>Healthy, privately owned dogs (n = 48) were prospectively recruited to the study. Blood samples were collected via direct jugular venipuncture for complete blood count, serum biochemistry analysis and measurement of prothrombin time (PT) and activated partial thromboplastin time (aPTT). Five animals with major abnormalities or who became agitated during phlebotomy were excluded. For the 43 remaining animals, 2 mL of blood was collected via the cephalic vein and added directly to a C-ACT tube that was shaken vigorously before being placed in a water bath at 37°C. Tubes were visually assessed for clot formation and C-ACT was recorded in seconds when the magnet within the tube lodged in the clot.</p><p><strong>Results: </strong>The nonparametric reference interval (capturing the central 95% of the data) was 50-80 seconds, with a 90% CI for the lower limit of 50-55 seconds and a 90% CI for the upper limit of 75-80 seconds. The C-ACT ACT test had a positive correlation with aPTT (0.42; 95% CI = 0.13-0.64). There was no evidence of a correlation between C-ACT ACT and age, weight, PT, haematocrit, white blood cell count, platelet count or total protein.</p><p><strong>Conclusions and clinical relevance: </strong>The results of this study suggest that the normal reference interval for ACT in dogs using C-ACT tubes in a 37°C water bath is 50-80 seconds. Care should be taken extrapolating the results of this study to the general population, as the smaller study design had less control for confounders than a larger study. However, when using the described analytical methods, C-ACT tube ACT test results >80 seconds should be considered prolonged in dogs and should prompt further investigation.</p>","PeriodicalId":19322,"journal":{"name":"New Zealand veterinary journal","volume":null,"pages":null},"PeriodicalIF":1.1000,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Reference interval for the C-ACT activated clotting time test in healthy dogs using a water bath and manual assessment of clot formation.\",\"authors\":\"K J Nash, S K Day, W A Goodwin\",\"doi\":\"10.1080/00480169.2023.2201222\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Aims: </strong>To establish a reference range for the canine C-ACT activated clotting time (ACT) test using a water bath and visual clot assessment technique.</p><p><strong>Methods: </strong>Healthy, privately owned dogs (n = 48) were prospectively recruited to the study. Blood samples were collected via direct jugular venipuncture for complete blood count, serum biochemistry analysis and measurement of prothrombin time (PT) and activated partial thromboplastin time (aPTT). Five animals with major abnormalities or who became agitated during phlebotomy were excluded. For the 43 remaining animals, 2 mL of blood was collected via the cephalic vein and added directly to a C-ACT tube that was shaken vigorously before being placed in a water bath at 37°C. Tubes were visually assessed for clot formation and C-ACT was recorded in seconds when the magnet within the tube lodged in the clot.</p><p><strong>Results: </strong>The nonparametric reference interval (capturing the central 95% of the data) was 50-80 seconds, with a 90% CI for the lower limit of 50-55 seconds and a 90% CI for the upper limit of 75-80 seconds. The C-ACT ACT test had a positive correlation with aPTT (0.42; 95% CI = 0.13-0.64). There was no evidence of a correlation between C-ACT ACT and age, weight, PT, haematocrit, white blood cell count, platelet count or total protein.</p><p><strong>Conclusions and clinical relevance: </strong>The results of this study suggest that the normal reference interval for ACT in dogs using C-ACT tubes in a 37°C water bath is 50-80 seconds. Care should be taken extrapolating the results of this study to the general population, as the smaller study design had less control for confounders than a larger study. However, when using the described analytical methods, C-ACT tube ACT test results >80 seconds should be considered prolonged in dogs and should prompt further investigation.</p>\",\"PeriodicalId\":19322,\"journal\":{\"name\":\"New Zealand veterinary journal\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.1000,\"publicationDate\":\"2023-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"New Zealand veterinary journal\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1080/00480169.2023.2201222\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"New Zealand veterinary journal","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1080/00480169.2023.2201222","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
引用次数: 0
摘要
目的:采用水浴法和目视凝块评估技术建立犬C-ACT活化凝血时间(ACT)试验的参考范围。方法:前瞻性地招募健康的私人养狗(n = 48)进行研究。直接颈静脉穿刺采血,进行全血细胞计数、血清生化分析、凝血酶原时间(PT)和部分凝血活酶活化时间(aPTT)测定。排除5只有重大异常或在放血过程中出现躁动的动物。其余43只动物经头静脉采集2ml血液,直接加入C- act管中,用力摇动后置于37℃水浴中。目视评估试管是否形成凝块,当试管内的磁铁进入凝块时,在几秒钟内记录C-ACT。结果:非参数参考区间(捕获中心95%的数据)为50-80秒,下限为50-55秒的CI为90%,上限为75-80秒的CI为90%。C-ACT ACT检验与aPTT呈正相关(0.42;95% ci = 0.13-0.64)。没有证据表明C-ACT与年龄、体重、PT、红细胞压积、白细胞计数、血小板计数或总蛋白之间存在相关性。结论及临床意义:本研究结果表明,在37°C水浴中使用C-ACT管的狗的正常参考时间间隔为50-80秒。将本研究的结果外推到一般人群时应谨慎,因为较小的研究设计比较大的研究对混杂因素的控制较少。然而,当使用上述分析方法时,C-ACT管ACT测试结果>80秒应被认为在犬中延长,应提示进一步研究。
Reference interval for the C-ACT activated clotting time test in healthy dogs using a water bath and manual assessment of clot formation.
Aims: To establish a reference range for the canine C-ACT activated clotting time (ACT) test using a water bath and visual clot assessment technique.
Methods: Healthy, privately owned dogs (n = 48) were prospectively recruited to the study. Blood samples were collected via direct jugular venipuncture for complete blood count, serum biochemistry analysis and measurement of prothrombin time (PT) and activated partial thromboplastin time (aPTT). Five animals with major abnormalities or who became agitated during phlebotomy were excluded. For the 43 remaining animals, 2 mL of blood was collected via the cephalic vein and added directly to a C-ACT tube that was shaken vigorously before being placed in a water bath at 37°C. Tubes were visually assessed for clot formation and C-ACT was recorded in seconds when the magnet within the tube lodged in the clot.
Results: The nonparametric reference interval (capturing the central 95% of the data) was 50-80 seconds, with a 90% CI for the lower limit of 50-55 seconds and a 90% CI for the upper limit of 75-80 seconds. The C-ACT ACT test had a positive correlation with aPTT (0.42; 95% CI = 0.13-0.64). There was no evidence of a correlation between C-ACT ACT and age, weight, PT, haematocrit, white blood cell count, platelet count or total protein.
Conclusions and clinical relevance: The results of this study suggest that the normal reference interval for ACT in dogs using C-ACT tubes in a 37°C water bath is 50-80 seconds. Care should be taken extrapolating the results of this study to the general population, as the smaller study design had less control for confounders than a larger study. However, when using the described analytical methods, C-ACT tube ACT test results >80 seconds should be considered prolonged in dogs and should prompt further investigation.
期刊介绍:
The New Zealand Veterinary Journal (NZVJ) is an international journal publishing high quality peer-reviewed articles covering all aspects of veterinary science, including clinical practice, animal welfare and animal health.
The NZVJ publishes original research findings, clinical communications (including novel case reports and case series), rapid communications, correspondence and review articles, originating from New Zealand and internationally.
Topics should be relevant to, but not limited to, New Zealand veterinary and animal science communities, and include the disciplines of infectious disease, medicine, surgery and the health, management and welfare of production and companion animals, horses and New Zealand wildlife.
All submissions are expected to meet the highest ethical and welfare standards, as detailed in the Journal’s instructions for authors.