基于crispr的可编程RNA操作与检测研究进展。

IF 6.4 2区 生物学 Q1 CELL BIOLOGY Wiley Interdisciplinary Reviews: RNA Pub Date : 2023-11-01 Epub Date: 2023-06-06 DOI:10.1002/wrna.1804
Beibei Wang, Hui Yang
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引用次数: 0

摘要

原核生物集群定期间隔的短回文重复序列和CRISPR相关(CRISPR- cas)系统通过rna引导的内切酶识别和消除入侵的外来核酸提供适应性免疫。II型Cas9、V型Cas12、VI型Cas13和III型Csm/Cmr复合物已被很好地表征并开发为可编程平台,用于选择性靶向和操纵原核和真核细胞中感兴趣的RNA分子。这些Cas效应物表现出显著的核糖核蛋白(RNP)组成、靶标识别和切割机制以及自我区分机制的多样性,可用于各种RNA靶向应用。在这里,我们总结了目前对这些Cas效应物的机制和功能特征的理解,概述了迄今为止建立的RNA检测和操作工具箱,包括敲除、编辑、成像、修饰和绘制RNA-蛋白相互作用,并讨论了基于crispr的RNA靶向工具的未来发展方向。本文分类如下:RNA方法>细胞中的RNA分析RNA加工> RNA编辑和修饰RNA与蛋白质和其他分子的相互作用>蛋白质-RNA相互作用:功能意义。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Progress of CRISPR-based programmable RNA manipulation and detection.

Prokaryotic clustered regularly interspaced short palindromic repeats and CRISPR associated (CRISPR-Cas) systems provide adaptive immunity by using RNA-guided endonucleases to recognize and eliminate invading foreign nucleic acids. Type II Cas9, type V Cas12, type VI Cas13, and type III Csm/Cmr complexes have been well characterized and developed as programmable platforms for selectively targeting and manipulating RNA molecules of interest in prokaryotic and eukaryotic cells. These Cas effectors exhibit remarkable diversity of ribonucleoprotein (RNP) composition, target recognition and cleavage mechanisms, and self discrimination mechanisms, which are leveraged for various RNA targeting applications. Here, we summarize the current understanding of mechanistic and functional characteristics of these Cas effectors, give an overview on RNA detection and manipulation toolbox established so far including knockdown, editing, imaging, modification, and mapping RNA-protein interactions, and discuss the future directions for CRISPR-based RNA targeting tools. This article is categorized under: RNA Methods > RNA Analyses in Cells RNA Processing > RNA Editing and Modification RNA Interactions with Proteins and Other Molecules > Protein-RNA Interactions: Functional Implications.

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来源期刊
CiteScore
14.80
自引率
4.10%
发文量
67
审稿时长
6-12 weeks
期刊介绍: WIREs RNA aims to provide comprehensive, up-to-date, and coherent coverage of this interesting and growing field, providing a framework for both RNA experts and interdisciplinary researchers to not only gain perspective in areas of RNA biology, but to generate new insights and applications as well. Major topics to be covered are: RNA Structure and Dynamics; RNA Evolution and Genomics; RNA-Based Catalysis; RNA Interactions with Proteins and Other Molecules; Translation; RNA Processing; RNA Export/Localization; RNA Turnover and Surveillance; Regulatory RNAs/RNAi/Riboswitches; RNA in Disease and Development; and RNA Methods.
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