ABCB1-1Δ基因突变在新西兰猎犬样本中的流行程度。

IF 1.1 4区 农林科学 Q3 VETERINARY SCIENCES New Zealand veterinary journal Pub Date : 2023-05-01 DOI:10.1080/00480169.2023.2181238
K Gedye, E Poole-Crowe, M Shepherd, A Wilding, K Parton, N Lopez-Villalobos, N Cave
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引用次数: 0

摘要

目的:确定ATP结合盒B亚家族成员1-1Δ突变的发生率(ABCB1-1Δ;在一群新西兰猎兔犬中发现了先前的耐多药1 (MDR1)突变。材料和方法:机会性地从新西兰各地的猎犬(n = 189)中收集样本。从怀拉拉帕地区的42名猎人中采集了口腔拭子,从吉斯本、怀卡托、马纳瓦特/旺格努伊、霍克斯湾、坎特伯雷和奥塔哥地区的猎人中采集了147份血液样本。从所有样本中提取DNA并检测ABCB1-1Δ等位基因的存在。结果:189只猎犬中有2只为ABCB1-1Δ等位基因的杂合携带者,其余187/189只猎犬为野生型等位基因的纯合携带者。未发现该突变的纯合犬。结论和临床意义:本研究结果表明,ABCB1-1Δ等位基因存在于猎犬中。该样本的低流行率表明,该等位基因在Huntaway人群中的流行率可能很低。我们建议兽医临床医生与狗主人讨论这种突变的可能性,包括对突变等位基因纯合子的狗的临床意义,以及对突变进行检测的可能性,就像他们对其他已知突变所做的那样。
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Prevalence of the ABCB1-1Δ gene mutation in a sample of New Zealand Huntaway dogs.

Aims: To determine the prevalence of the ATP Binding Cassette Subfamily B Member 1-1Δ mutation (ABCB1-1Δ; previously Multidrug Resistance 1 (MDR1) mutation) in a cohort of New Zealand Huntaway dogs.

Materials and methods: Samples were opportunistically collected from Huntaway dogs (n = 189) from throughout New Zealand. Buccal swabs were collected from 42 Huntaways from the Wairarapa region and 147 blood samples from Huntaways from the Gisborne, Waikato, Manawatū/Whanganui, Hawkes Bay, Canterbury and Otago regions. DNA was extracted from all samples and tested for the presence of the ABCB1-1Δ allele.

Results: Of 189 Huntaway dogs that were tested, two were found to be heterozygous carriers of the ABCB1-1Δ allele and the remaining 187/189 dogs were homozygous for the wild type allele. No dogs homozygous for the mutation were identified.

Conclusions and clinical relevance: The results of this study show that the ABCB1-1Δ allele is present in Huntaway dogs. The low prevalence in this convenience sample suggests that the prevalence of this allele in the Huntaway population is likely to be low. We recommend that veterinary clinicians discuss the potential for this mutation in Huntaways with dog owners including the clinical implications for dogs that are homozygous for the mutated allele and the potential for testing for the mutation, as they would do for other known mutations.

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来源期刊
New Zealand veterinary journal
New Zealand veterinary journal 农林科学-兽医学
CiteScore
3.00
自引率
0.00%
发文量
37
审稿时长
12-24 weeks
期刊介绍: The New Zealand Veterinary Journal (NZVJ) is an international journal publishing high quality peer-reviewed articles covering all aspects of veterinary science, including clinical practice, animal welfare and animal health. The NZVJ publishes original research findings, clinical communications (including novel case reports and case series), rapid communications, correspondence and review articles, originating from New Zealand and internationally. Topics should be relevant to, but not limited to, New Zealand veterinary and animal science communities, and include the disciplines of infectious disease, medicine, surgery and the health, management and welfare of production and companion animals, horses and New Zealand wildlife. All submissions are expected to meet the highest ethical and welfare standards, as detailed in the Journal’s instructions for authors.
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