资源有限环境下的全基因组测序:挑战与机遇。

IF 3.1 Q2 MICROBIOLOGY Current Clinical Microbiology Reports Pub Date : 2022-01-01 DOI:10.1007/s40588-022-00183-1
Fredrickson B Wasswa, Kennedy Kassaza, Kirsten Nielsen, Joel Bazira
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引用次数: 0

摘要

综述目的:MinION全基因组测序技术的引入极大地促进和简化了全球各个科学领域的全基因组测序。从复杂生物体到微生物的序列已经产生,并存储在基因组数据库中,便于研究人员访问。各种用于基因组分析的新软件,以及对旧软件包的升级,正在产生。新的协议也正在得到验证,使WGS技术能够迅速并越来越多地用于现场测序。MinION WGS技术由于其便携性、实时分析和与其他测序技术相比成本更低的优点,已在发达国家实施。虽然这些同样的优势在发展中国家至关重要,但在资源有限的情况下,MinION WGS技术仍未得到充分利用。摘要:在这篇综述中,我们着眼于在资源有限的环境中使用MinION WGS的应用、优势、挑战和机遇。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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MinION Whole-Genome Sequencing in Resource-Limited Settings: Challenges and Opportunities.

Purpose of review: The introduction of MinION whole-genome sequencing technology greatly increased and simplified complete genome sequencing in various fields of science across the globe. Sequences have been generated from complex organisms to microorganisms and are stored in genome databases that are readily accessible by researchers. Various new software for genome analysis, along with upgrades to older software packages, are being generated. New protocols are also being validated that enable WGS technology to be rapidly and increasingly used for sequencing in field settings.

Recent findings: MinION WGS technology has been implemented in developed countries due to its advantages: portability, real-time analysis, and lower cost compared to other sequencing technologies. While these same advantages are critical in developing countries, MinION WGS technology is still under-utilized in resource-limited settings.

Summary: In this review, we look at the applications, advantages, challenges, and opportunities of using MinION WGS in resource-limited settings.

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来源期刊
CiteScore
7.50
自引率
1.90%
发文量
9
期刊介绍: Current Clinical Microbiology Reports commissions expert reviews from leading scientists at the forefront of research in microbiology. The journal covers this broad field by dividing it into four key main areas of study: virology, bacteriology, parasitology, and mycology. Within each of the four sections, experts from around the world address important aspects of clinical microbiology such as immunology, diagnostics, therapeutics, antibiotics and antibiotic resistance, and vaccines. Some of the world’s foremost authorities in the field of microbiology serve as section editors and editorial board members. Section editors select topics for which leading researchers are invited to contribute comprehensive review articles that emphasize new developments and recently published papers of major importance, which are highlighted in annotated reference lists. These timely reviews of the literature examine the latest scientific discoveries and controversies as they emerge and are indispensable to both researchers and clinicians. The editorial board, composed of more than 20 internationally diverse members, reviews the annual table of contents, ensures that topics address all aspects of emerging research, and where applicable suggests topics of critical importance to various countries/regions.
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