IncRNA MALAT1通过靶向miR-873-5p/ROCK1调控骨肉瘤细胞的增殖、凋亡、迁移和侵袭

IF 1.5 4区 医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Critical Reviews in Eukaryotic Gene Expression Pub Date : 2023-01-01 DOI:10.1615/CritRevEukaryotGeneExpr.2022044747
Fan Yang, Mao Wang, Junlong Shi, Gang Xu
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引用次数: 3

摘要

骨恶性肿瘤骨肉瘤(osteosarcoma, OS)是最具侵袭性的肿瘤之一。尽管最近在OS的治疗选择上取得了突破,但在过去的25年里,转移或复发疾病患者的生存率保持不变,约为20%。lncRNA表达失调与癌症发生、进展和转移有关。此外,lncRNAs调控OS细胞生物活性和进展的基本机制仍在研究中。采用实时定量聚合酶链反应(qRT-PCR)检测OS中miR-873-5p和MALAT1的表达。通过Kaplan-Meier绘图仪评估MALAT1表达水平与OS个体生存率的关系。采用CCK-8检测肿瘤细胞的增殖能力。Transwell用于检测肿瘤细胞的迁移和侵袭特性。western blot检测ROCK1蛋白表达,qRT-PCR检测ROCK1 mRNA表达。利用StarBase2.0预测MALAT1或miR-873-5p的靶基因。通过荧光素酶测定证实miR-873-5p与MALAT1或ROCK1之间的靶标关联。采用Spearman相关分析研究ROCK1与OS中MALAT1或miR-873-5p表达的关系。MALAT1表达上调,与OS患者较低的生存率有关。下调MALAT1可抑制细胞的恶性行为。双荧光素酶基因实验证实存在MALAT1/miR-873-5p/ROCK1轴。上调的miR-873-5p阻断了MALAT1对细胞行为的促进作用。过表达MALAT1可通过miR-873-5p/ROCK1轴促进OS细胞的恶性行为。
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IncRNA MALAT1 Regulates the Proliferation, Apoptosis, Migration, and Invasion of Osteosarcoma Cells by Targeting miR-873-5p/ROCK1.

The malignant bone tumor osteosarcoma (OS) was one of the most aggressive tumors. Despite breakthroughs in treatment options for OS recently, the survival rate of patients with metastasis or reoccurring disease has remained unchanged over the last 25 years, at around 20%. lncRNA expression dysregulation is linked to carcinogenesis, advancement, and metastasis. Additionally, the fundamental mechanism of lncRNAs in regulating OS cell biological activity and progression is still being investigated. The expression of miR-873-5p and MALAT1 were detected by quantitative real-time polymerase chain reaction (qRT-PCR) in OS. The relationship between the expression level of MALAT1 and the survival rate of OS individuals was evaluated by the Kaplan-Meier plotter. The tumor cell's capability of proliferation was determined using the CCK-8. Transwell was used to test the migratory and invasive properties of tumor cells. ROCK1 protein expression was analyzed by western blot, while qRT-PCR was used to detect ROCK1 mRNA expression. Targeted genes of MALAT1 or miR-873-5p were predicted by StarBase2.0. The target association among miR-873-5p and MALAT1 or ROCK1 was confirmed using the luciferase assay. The relationship between ROCK1 and MALAT1 or miR-873-5p expression in OS was investigated using Spearman's correlation analysis. MALAT1 was up-regulated and was linked to a lower survival rate of patients in OS. The malignant behaviors of cells were inhibited by down-regulated MALAT1 in vitro. Dual-luciferase gene experiments confirmed the presence of MALAT1/miR-873-5p/ROCK1 axis. The up-regulated miR-873-5p blocked the promoted effects of MALAT1 on cell behaviors. Over-expressed MALAT1 promoted the malignant behaviors of cells by miR-873-5p/ROCK1 axis in OS.

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来源期刊
Critical Reviews in Eukaryotic Gene Expression
Critical Reviews in Eukaryotic Gene Expression 生物-生物工程与应用微生物
CiteScore
2.70
自引率
0.00%
发文量
67
审稿时长
1 months
期刊介绍: Critical ReviewsTM in Eukaryotic Gene Expression presents timely concepts and experimental approaches that are contributing to rapid advances in our mechanistic understanding of gene regulation, organization, and structure within the contexts of biological control and the diagnosis/treatment of disease. The journal provides in-depth critical reviews, on well-defined topics of immediate interest, written by recognized specialists in the field. Extensive literature citations provide a comprehensive information resource. Reviews are developed from an historical perspective and suggest directions that can be anticipated. Strengths as well as limitations of methodologies and experimental strategies are considered.
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