SARS-CoV-2/COVID-19 时代的数字 PCR 应用:未来疫情爆发路线图。

IF 19 1区 医学 Q1 MICROBIOLOGY Clinical Microbiology Reviews Pub Date : 2022-09-21 Epub Date: 2022-03-08 DOI:10.1128/cmr.00168-21
Raphael Nyaruaba, Caroline Mwaliko, David Dobnik, Pavel Neužil, Patrick Amoth, Matilu Mwau, Junping Yu, Hang Yang, Hongping Wei
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引用次数: 0

摘要

由严重急性呼吸系统综合征冠状病毒 2(SARS-CoV-2)引起的 2019 年冠状病毒病(COVID-19)大流行导致了一场全球性的公共卫生灾难。目前诊断感染患者的金标准是实时反转录定量 PCR(RT-qPCR)。这种方法虽然有效,但容易出现假阴性和假阳性结果,影响其精确性,尤其是在检测样本中的低病毒载量时。相比之下,数字 PCR(dPCR)作为第三代 PCR,在检测样本中的低病毒载量方面已被证明比金标准 RT-qPCR 更有效。在这篇综述文章中,我们选取了一些出版物来展示 dPCR 的广泛应用,包括检测方法和参考标准的开发、环境监测、突变检测以及 SARS-CoV-2 的临床诊断,同时将其与黄金标准 RT-qPCR 进行了分析比较。总之,RT-dPCR 的特异性、灵敏度、可重复性和检测限一般不受可能影响 RT-qPCR 的常见因素的影响。由于这是首次在如此大规模的疫情中对 dPCR 进行测试,因此了解其应用情况将有助于为今后诊断和监测传染病疫情指明方向。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Digital PCR Applications in the SARS-CoV-2/COVID-19 Era: a Roadmap for Future Outbreaks.

The ongoing coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has led to a global public health disaster. The current gold standard for the diagnosis of infected patients is real-time reverse transcription-quantitative PCR (RT-qPCR). As effective as this method may be, it is subject to false-negative and -positive results, affecting its precision, especially for the detection of low viral loads in samples. In contrast, digital PCR (dPCR), the third generation of PCR, has been shown to be more effective than the gold standard, RT-qPCR, in detecting low viral loads in samples. In this review article, we selected publications to show the broad-spectrum applications of dPCR, including the development of assays and reference standards, environmental monitoring, mutation detection, and clinical diagnosis of SARS-CoV-2, while comparing it analytically to the gold standard, RT-qPCR. In summary, it is evident that the specificity, sensitivity, reproducibility, and detection limits of RT-dPCR are generally unaffected by common factors that may affect RT-qPCR. As this is the first time that dPCR is being tested in an outbreak of such a magnitude, knowledge of its applications will help chart a course for future diagnosis and monitoring of infectious disease outbreaks.

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来源期刊
Clinical Microbiology Reviews
Clinical Microbiology Reviews 医学-微生物学
CiteScore
54.20
自引率
0.50%
发文量
38
期刊介绍: Clinical Microbiology Reviews (CMR) is a journal that primarily focuses on clinical microbiology and immunology.It aims to provide readers with up-to-date information on the latest developments in these fields.CMR also presents the current state of knowledge in clinical microbiology and immunology.Additionally, the journal offers balanced and thought-provoking perspectives on controversial issues in these areas.
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