番红花和氯沙坦对糖尿病肾病大鼠肾组织TGF-β基因表达和组织病理学的影响。

IF 1.9 Q3 CHEMISTRY, MEDICINAL Avicenna Journal of Phytomedicine Pub Date : 2023-03-01 DOI:10.22038/AJP.2022.21414
Yaser Mohammadi, Mohammad Zangooei, Mahmoud Zardast, Morteza Mamashli, Azam Rezaei Farimani
{"title":"番红花和氯沙坦对糖尿病肾病大鼠肾组织TGF-β基因表达和组织病理学的影响。","authors":"Yaser Mohammadi,&nbsp;Mohammad Zangooei,&nbsp;Mahmoud Zardast,&nbsp;Morteza Mamashli,&nbsp;Azam Rezaei Farimani","doi":"10.22038/AJP.2022.21414","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>Diabetic nephropathy is one of the most common microvascular complications of diabetes mellitus that finally leads to complete loss of kidney function. Therefore, this study aimed to evaluate the effect of crocin and losartan on <i>TGF-β</i> gene expression and histopathology of kidney tissue in a rat model of diabetic nephropathy.</p><p><strong>Materials and methods: </strong>Forty male Wistar rats were randomly divided into five groups (n=8): Untreated control, Diabetic (D), D + crocin, D + losartan, and D + losartan + crocin. Induction of diabetes was performed using streptozotocin (50 mg/kg/ Intraperitoneal injection). At the end of the eight-week period, the rats were sacrificed. Spectrophotometry measured serum glucose, urea, creatinine, and uric acid levels. Microalbumin and creatinine levels were measured in 24-hour urine. Real-time PCR was used to determine the relative expression of the <i>TGF-β</i> gene in kidney tissue. Renal tissue histopathology was also examined.</p><p><strong>Results: </strong>The results showed that hyperglycemia increased biochemical factors associated with diabetes, <i>TGF-β</i> gene expression, and kidney damage. Separate treatment with crocin and losartan led to a decrease in renal function factors and <i>TGF-β</i> gene expression and improved kidney damage.</p><p><strong>Conclusion: </strong>Our results showed that crocin could improve kidney function in diabetic conditions. In addition, we showed that crocin increases the effectiveness of losartan. Consequently, we suggest that crocin in combination with chemical drugs can be a potential therapeutic agent for diabetes and its complications. Nonetheless, human studies are needed to make firm findings.</p>","PeriodicalId":8677,"journal":{"name":"Avicenna Journal of Phytomedicine","volume":null,"pages":null},"PeriodicalIF":1.9000,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10274314/pdf/","citationCount":"1","resultStr":"{\"title\":\"The effect of crocin and losartan on <i>TGF-β</i> gene expression and histopathology of kidney tissue in a rat model of diabetic nephropathy.\",\"authors\":\"Yaser Mohammadi,&nbsp;Mohammad Zangooei,&nbsp;Mahmoud Zardast,&nbsp;Morteza Mamashli,&nbsp;Azam Rezaei Farimani\",\"doi\":\"10.22038/AJP.2022.21414\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>Diabetic nephropathy is one of the most common microvascular complications of diabetes mellitus that finally leads to complete loss of kidney function. Therefore, this study aimed to evaluate the effect of crocin and losartan on <i>TGF-β</i> gene expression and histopathology of kidney tissue in a rat model of diabetic nephropathy.</p><p><strong>Materials and methods: </strong>Forty male Wistar rats were randomly divided into five groups (n=8): Untreated control, Diabetic (D), D + crocin, D + losartan, and D + losartan + crocin. Induction of diabetes was performed using streptozotocin (50 mg/kg/ Intraperitoneal injection). At the end of the eight-week period, the rats were sacrificed. Spectrophotometry measured serum glucose, urea, creatinine, and uric acid levels. Microalbumin and creatinine levels were measured in 24-hour urine. Real-time PCR was used to determine the relative expression of the <i>TGF-β</i> gene in kidney tissue. Renal tissue histopathology was also examined.</p><p><strong>Results: </strong>The results showed that hyperglycemia increased biochemical factors associated with diabetes, <i>TGF-β</i> gene expression, and kidney damage. Separate treatment with crocin and losartan led to a decrease in renal function factors and <i>TGF-β</i> gene expression and improved kidney damage.</p><p><strong>Conclusion: </strong>Our results showed that crocin could improve kidney function in diabetic conditions. In addition, we showed that crocin increases the effectiveness of losartan. Consequently, we suggest that crocin in combination with chemical drugs can be a potential therapeutic agent for diabetes and its complications. Nonetheless, human studies are needed to make firm findings.</p>\",\"PeriodicalId\":8677,\"journal\":{\"name\":\"Avicenna Journal of Phytomedicine\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.9000,\"publicationDate\":\"2023-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10274314/pdf/\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Avicenna Journal of Phytomedicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.22038/AJP.2022.21414\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"CHEMISTRY, MEDICINAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Avicenna Journal of Phytomedicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.22038/AJP.2022.21414","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, MEDICINAL","Score":null,"Total":0}
引用次数: 1

摘要

目的:糖尿病肾病是糖尿病最常见的微血管并发症之一,最终导致肾功能完全丧失。因此,本研究旨在评估番红花和氯沙坦对糖尿病肾病大鼠模型肾组织TGF-β基因表达和组织病理学的影响。材料和方法:雄性Wistar大鼠40只,随机分为5组(n=8):未治疗对照组、糖尿病组(D)、D+克罗辛组、D+氯沙坦组和D+氯沙坦+克罗辛。使用链脲佐菌素(50mg/kg/腹膜内注射)诱导糖尿病。在八周结束时,处死大鼠。分光光度法测量血清葡萄糖、尿素、肌酸酐和尿酸水平。测量24小时尿液中的微量白蛋白和肌酐水平。实时聚合酶链式反应检测肾组织中TGF-β基因的相对表达。还检查了肾组织病理学。结果:高血糖增加了与糖尿病、TGF-β基因表达和肾损伤相关的生化因子。单独使用番红花苷和氯沙坦治疗可降低肾功能因子和TGF-β基因表达,改善肾损伤。结论:番红花苷可改善糖尿病患者的肾功能。此外,我们发现番红花苷能提高氯沙坦的疗效。因此,我们认为番红花苷与化学药物联合治疗糖尿病及其并发症可能是一种潜在的治疗剂。尽管如此,还是需要进行人类研究才能得出确切的结论。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

摘要图片

摘要图片

摘要图片

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
The effect of crocin and losartan on TGF-β gene expression and histopathology of kidney tissue in a rat model of diabetic nephropathy.

Objective: Diabetic nephropathy is one of the most common microvascular complications of diabetes mellitus that finally leads to complete loss of kidney function. Therefore, this study aimed to evaluate the effect of crocin and losartan on TGF-β gene expression and histopathology of kidney tissue in a rat model of diabetic nephropathy.

Materials and methods: Forty male Wistar rats were randomly divided into five groups (n=8): Untreated control, Diabetic (D), D + crocin, D + losartan, and D + losartan + crocin. Induction of diabetes was performed using streptozotocin (50 mg/kg/ Intraperitoneal injection). At the end of the eight-week period, the rats were sacrificed. Spectrophotometry measured serum glucose, urea, creatinine, and uric acid levels. Microalbumin and creatinine levels were measured in 24-hour urine. Real-time PCR was used to determine the relative expression of the TGF-β gene in kidney tissue. Renal tissue histopathology was also examined.

Results: The results showed that hyperglycemia increased biochemical factors associated with diabetes, TGF-β gene expression, and kidney damage. Separate treatment with crocin and losartan led to a decrease in renal function factors and TGF-β gene expression and improved kidney damage.

Conclusion: Our results showed that crocin could improve kidney function in diabetic conditions. In addition, we showed that crocin increases the effectiveness of losartan. Consequently, we suggest that crocin in combination with chemical drugs can be a potential therapeutic agent for diabetes and its complications. Nonetheless, human studies are needed to make firm findings.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Avicenna Journal of Phytomedicine
Avicenna Journal of Phytomedicine CHEMISTRY, MEDICINAL-
CiteScore
3.40
自引率
4.50%
发文量
17
审稿时长
6 weeks
期刊最新文献
Aloe vera supplementation improves cardiovascular risk factors in hemodialysis patients: A randomized, double-blind, placebo-controlled trial. An in vitro antiviral evaluation of punicalagin toward influenza A virus. Apigenin attenuates serum concentrations of TNF-a, interleukin 1b and interleukin 6 in lipopolysaccharide-stimulated rats. Comparison of effects of P-coumaric acid and coumarin on colorectal cancer cell line by inducing apoptosis and autophagy. Effect of pretreatment with Devil's Claw on locomotor activity, infarct volume, and neuronal density in focal cerebral ischemia in rats.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1