用于慢性HBV感染患者不同表面基因变异模式扩增和测定的半巢式PCR技术的开发和评价:HBV S基因扩增方法的开发。

Q3 Medicine Gastroenterology and Hepatology From Bed to Bench Pub Date : 2023-01-01
Fedra Mokhtari, Hami Kaboosi, Seyed Reza Mohebbi, Hamid Asadzadeh Aghdaei, Mohammad Reza Zali
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引用次数: 0

摘要

目的:建立一种进一步研究HBV S基因变异模式的扩增方法。背景:慢性HBV感染患者的Pre-S/S变异可能促进肝损伤和肝细胞癌(HCC)的进展。方法:对10例慢性HBV感染患者进行研究。从患者血浆中提取病毒DNA,进行引物设计,建立半巢式PCR扩增HBV基因组前S/S区方法。随后,对该区域的变异进行测序分析。结果:本研究成功建立了半巢式PCR方法,并对所研究样品的变异类型进行了研究。结论:应在HBV携带者中常规检测Pre-S/S变异,以帮助识别可能处于较不利肝病进展高风险的个体。本研究表明,该技术能够准确扩增前S/S区,产品可成功用于直接测序的变异检测。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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Development and evaluation of a semi-nested PCR technique for amplification and determination of different surface gene variation patterns in patients with chronic HBV infection: development of an amplification method for HBV S gene.

Aim: Development of an amplification method for further investigation of HBV S gene variation patterns.

Background: Pre-S/S variants in patients with chronic HBV infection may contribute to the progression of liver damage and Hepatocellular carcinoma (HCC).

Methods: This study was performed on ten patients with chronic HBV infection. Viral DNA was extracted from patient's plasma, primer design was performed, and a semi-nested PCR method was set up to amplify the pre-S/S region of HBV genome. Subsequently, sequencing was performed to analyze the variants of this region.

Results: In the current study, the semi-nested PCR method was successfully set up, and types of variation in the studied samples were investigated.

Conclusion: Pre-S/S variants should be routinely determined in HBV carriers to help identify individuals who may be at a high risk of less favorable liver disease progression. This study showed that the technique could accurately amplify the pre-S/S region, and the product can be successfully used for variation detection by direct sequencing.

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CiteScore
2.30
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0.00%
发文量
29
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