蜱细胞感染尼帕病毒的实验。

IF 0.8 4区医学 Q4 PARASITOLOGY Tropical biomedicine Pub Date : 2023-03-01 DOI:10.47665/tb.40.1.009

W H Phoon, L Bell-Sakyi, S AbuBakar, L Y Chang

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引用次数: 0

摘要

尼帕病毒（NiV）是副粘病毒科的一种高致病性鸡病毒，可导致 40-70% 的患者患上致命性脑炎，20 多年前首次在马来西亚被报道。翼手目蝙蝠是鸡病毒的天然宿主，有人提出蜱可能是蝙蝠和哺乳动物宿主之间的联系。为了研究这一假设，我们对蜱细胞系 IDE8 感染 NiV 的情况进行了研究。经证实，受 NiV 感染的蜱细胞中存在病毒 RNA 和抗原。从感染了 NiV 的蜱细胞中回收了传染性病毒，并通过电子显微镜观察到了 NiV 的超微结构特征。这些结果表明，蜱可支持NiV感染，并可能在传播中发挥作用。

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Experimental infection of tick cells with Nipah virus.

Nipah virus (NiV), a highly pathogenic henipavirus of the family Paramyxoviridae, which causes fatal encephalitis in 40-70% of affected patients, was first reported in Malaysia over 20 years ago. Pteropid bats are the natural hosts of henipaviruses, and ticks have been proposed as a possible link between bats and mammalian hosts. To investigate this hypothesis, infection of the tick cell line IDE8 with NiV was examined. Presence of viral RNA and antigen in the NiV-infected tick cells was confirmed. Infectious virions were recovered from NiV-infected tick cells and ultrastructural features of NiV were observed by electron microscopy. These results suggest that ticks could support NiV infection, potentially playing a role in transmission.

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来源期刊

Tropical biomedicine 医学-寄生虫学

CiteScore

1.60

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： The Society publishes the Journal – Tropical Biomedicine, 4 issues yearly. It was first started in 1984. The journal is now abstracted / indexed by Medline, ISI Thompson, CAB International, Zoological Abstracts, SCOPUS. It is available free on the MSPTM website. Members may submit articles on Parasitology, Tropical Medicine and other related subjects for publication in the journal subject to scrutiny by referees. There is a charge of US$200 per manuscript. However, charges will be waived if the first author or corresponding author are members of MSPTM of at least three (3) years'' standing.