脂质翻转酶murp的结构与机制

IF 12.1 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Annual review of biochemistry Pub Date : 2022-06-21 DOI:10.1146/annurev-biochem-040320-105145
Alvin C Y Kuk, Aili Hao, Seok-Yong Lee
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引用次数: 6

摘要

许多重要的多糖(包括肽聚糖、脂多糖和n链聚糖)的生物合成需要脂链低聚糖(LLO)从它们的细胞质合成位点跨膜运输到它们的利用位点。我们目前对低聚糖转运的理解大部分来自多药/寡糖脂/多糖(MOP)超家族蛋白MurJ的遗传、生化和结构研究,该蛋白翻转肽聚糖前体脂质II。MurJ在细菌细胞壁合成中起着关键作用,是一种新兴的抗生素靶点。本文综述了MurJ翻转LLO的机制,包括脂质翻转和离子偶联的结构基础。然后,我们讨论了包括humimycin和噬菌体M裂解蛋白在内的抗菌素对MurJ的抑制作用,以及MurJ的研究如何为MOP超家族内外的其他翻转酶提供见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Structure and Mechanism of the Lipid Flippase MurJ.

Biosynthesis of many important polysaccharides (including peptidoglycan, lipopolysaccharide, and N-linked glycans) necessitates the transport of lipid-linked oligosaccharides (LLO) across membranes from their cytosolic site of synthesis to their sites of utilization. Much of our current understanding of LLO transport comes from genetic, biochemical, and structural studies of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) superfamily protein MurJ, which flips the peptidoglycan precursor lipid II. MurJ plays a pivotal role in bacterial cell wall synthesis and is an emerging antibiotic target. Here, we review the mechanism of LLO flipping by MurJ, including the structural basis for lipid II flipping and ion coupling. We then discuss inhibition of MurJ by antibacterials, including humimycins and the phage M lysis protein, as well as how studies on MurJ could provide insight into other flippases, both within and beyond the MOP superfamily.

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来源期刊
Annual review of biochemistry
Annual review of biochemistry 生物-生化与分子生物学
CiteScore
33.90
自引率
0.00%
发文量
31
期刊介绍: The Annual Review of Biochemistry, in publication since 1932, sets the standard for review articles in biological chemistry and molecular biology. Since its inception, these volumes have served as an indispensable resource for both the practicing biochemist and students of biochemistry.
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