卵形金刺草和寻常蒿提取物可抑制age介导的RAGE表达、ROS生成和THP-1细胞的炎症反应。

IF 1.6 4区 医学 Q4 TOXICOLOGY Toxicological Research Pub Date : 2022-07-01 DOI:10.1007/s43188-021-00114-0
Suporn Sukjamnong, Hui Chen, Sonia Saad, Rachana Santiyanont
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引用次数: 4

摘要

晚期糖基化终产物(AGEs)可以通过AGEs受体(RAGE)诱导炎症信号通路。靶向RAGE可能是治疗由AGE-RAGE轴介导的慢性炎症的一种治疗策略。本研究旨在探讨卵泡金霉和寻常蒿提取物对AGE-RAGE信号通路及age介导的THP-1细胞氧化应激和炎症的影响。采用索氏提取法提取枸杞和乌桕,用DPPH和ABTS测定其抗氧化能力。用AGE(600µg/ml)处理人单核细胞系THP-1, AGE加或不加卵泡草和寻常草提取物(100µg/ml)。以线粒体靶向抗氧化剂MitoQ (2 μg/ml)作为阳性对照。分析细胞活力、ROS生成、RAGE、AGE-RAGE信号通路成分和炎症细胞因子水平。在非细胞基础实验中,卵泡草和寻常草提取物显示出抗氧化作用。AGE处理THP-1细胞后,RAGE蛋白水平显著升高,TNF-α、IL-1β、IL-6等细胞因子mRNA表达显著升高。AGEs诱导了ROS的产生和RAGE下游信号分子的水平,包括磷酸化和总Erk1/2、JNK和p38 MAPK,尽管不显著。蛋清和荆芥提取物显著降低了RAGE蛋白水平,显著降低了细胞因子mRNA水平。综上所述,本研究揭示了卵泡草和寻常草提取物通过AGE-RAGE轴发挥抗炎作用。然而,这种通过AGE-RAGE信号传导的抗炎作用的细节还有待进一步研究。补充信息:在线版本包含补充资料,可在10.1007/s43188-021-00114-0获得。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Fimbristylis ovata and Artemisia vulgaris extracts inhibited AGE-mediated RAGE expression, ROS generation, and inflammation in THP-1 cells.

Advanced glycation end products (AGEs) can induce inflammatory signaling pathways through the receptor for AGEs (RAGE). Targeting RAGE could be a therapeutic strategy for treating chronic inflammation mediated by the AGE-RAGE axis. This study aimed to investigate the effects of Fimbristylis ovata and Artemisia vulgaris extracts on AGE-RAGE signaling and AGE-mediated oxidative stress and inflammation in THP-1 cells. F. ovata and A. vulgaris were extracted by a Soxhlet extraction, and antioxidant capacity was evaluated using DPPH and ABTS assays. The human monocytic cell line THP-1 was treated with AGE (600 µg/ml) with and without F. ovata and A. vulgaris extracts (100 µg/ml). The mitochondria-targeting antioxidant MitoQ (2 μg/ml) was used as a positive control. Cell viability, ROS generation, RAGE, AGE-RAGE signaling pathway components, and inflammatory cytokine levels were analyzed. F. ovata and A. vulgaris extracts showed antioxidative effects in non-cell-based assays. Treatment of THP-1 cells with AGE significantly increased the protein levels of RAGE and significantly increased the mRNA expression of cytokines, including TNF-α, IL-1β, and IL-6. AGEs induced the generation of ROS and levels of signaling molecules downstream of RAGE, including phosphorylated and total Erk1/2, JNK, and p38 MAPK, although not significantly. F. ovata and A. vulgaris extracts significantly decreased the protein levels of RAGE and significantly decreased the mRNA levels of cytokines. In conclusion, this study revealed that F. ovata and A. vulgaris extracts exert anti-inflammatory effects through the AGE-RAGE axis. However, details on this anti-inflammatory effect through AGE-RAGE signaling should be further investigated.

Supplementary information: The online version contains supplementary material available at 10.1007/s43188-021-00114-0.

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来源期刊
CiteScore
4.20
自引率
4.30%
发文量
39
期刊介绍: Toxicological Research is the official journal of the Korean Society of Toxicology. The journal covers all areas of Toxicological Research of chemicals, drugs and environmental agents affecting human and animals, which in turn impact public health. The journal’s mission is to disseminate scientific and technical information on diverse areas of toxicological research. Contributions by toxicologists, molecular biologists, geneticists, biochemists, pharmacologists, clinical researchers and epidemiologists with a global view on public health through toxicological research are welcome. Emphasis will be given to articles providing an understanding of the toxicological mechanisms affecting animal, human and public health. In the case of research articles using natural extracts, detailed information with respect to the origin, extraction method, chemical profiles, and characterization of standard compounds to ensure the reproducible pharmacological activity should be provided.
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