{"title":"卵形金刺草和寻常蒿提取物可抑制age介导的RAGE表达、ROS生成和THP-1细胞的炎症反应。","authors":"Suporn Sukjamnong, Hui Chen, Sonia Saad, Rachana Santiyanont","doi":"10.1007/s43188-021-00114-0","DOIUrl":null,"url":null,"abstract":"<p><p>Advanced glycation end products (AGEs) can induce inflammatory signaling pathways through the receptor for AGEs (RAGE). Targeting RAGE could be a therapeutic strategy for treating chronic inflammation mediated by the AGE-RAGE axis. This study aimed to investigate the effects of <i>Fimbristylis</i> <i>ovata</i> and <i>Artemisia</i> <i>vulgaris</i> extracts on AGE-RAGE signaling and AGE-mediated oxidative stress and inflammation in THP-1 cells. <i>F.</i> <i>ovata</i> and <i>A.</i> <i>vulgaris</i> were extracted by a Soxhlet extraction, and antioxidant capacity was evaluated using DPPH and ABTS assays. The human monocytic cell line THP-1 was treated with AGE (600 µg/ml) with and without <i>F.</i> <i>ovata</i> and <i>A.</i> <i>vulgaris</i> extracts (100 µg/ml). The mitochondria-targeting antioxidant MitoQ (2 μg/ml) was used as a positive control. Cell viability, ROS generation, RAGE, AGE-RAGE signaling pathway components, and inflammatory cytokine levels were analyzed. <i>F.</i> <i>ovata</i> and <i>A.</i> <i>vulgaris</i> extracts showed antioxidative effects in non-cell-based assays. Treatment of THP-1 cells with AGE significantly increased the protein levels of RAGE and significantly increased the mRNA expression of cytokines, including TNF-α, IL-1β, and IL-6. AGEs induced the generation of ROS and levels of signaling molecules downstream of RAGE, including phosphorylated and total Erk1/2, JNK, and p38 MAPK, although not significantly. <i>F.</i> <i>ovata</i> and <i>A.</i> <i>vulgaris</i> extracts significantly decreased the protein levels of RAGE and significantly decreased the mRNA levels of cytokines. In conclusion, this study revealed that <i>F.</i> <i>ovata</i> and <i>A.</i> <i>vulgaris</i> extracts exert anti-inflammatory effects through the AGE-RAGE axis. However, details on this anti-inflammatory effect through AGE-RAGE signaling should be further investigated.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s43188-021-00114-0.</p>","PeriodicalId":23181,"journal":{"name":"Toxicological Research","volume":"38 3","pages":"331-343"},"PeriodicalIF":1.6000,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9247136/pdf/43188_2021_Article_114.pdf","citationCount":"4","resultStr":"{\"title\":\"<i>Fimbristylis</i> <i>ovata</i> and <i>Artemisia</i> <i>vulgaris</i> extracts inhibited AGE-mediated RAGE expression, ROS generation, and inflammation in THP-1 cells.\",\"authors\":\"Suporn Sukjamnong, Hui Chen, Sonia Saad, Rachana Santiyanont\",\"doi\":\"10.1007/s43188-021-00114-0\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Advanced glycation end products (AGEs) can induce inflammatory signaling pathways through the receptor for AGEs (RAGE). Targeting RAGE could be a therapeutic strategy for treating chronic inflammation mediated by the AGE-RAGE axis. This study aimed to investigate the effects of <i>Fimbristylis</i> <i>ovata</i> and <i>Artemisia</i> <i>vulgaris</i> extracts on AGE-RAGE signaling and AGE-mediated oxidative stress and inflammation in THP-1 cells. <i>F.</i> <i>ovata</i> and <i>A.</i> <i>vulgaris</i> were extracted by a Soxhlet extraction, and antioxidant capacity was evaluated using DPPH and ABTS assays. The human monocytic cell line THP-1 was treated with AGE (600 µg/ml) with and without <i>F.</i> <i>ovata</i> and <i>A.</i> <i>vulgaris</i> extracts (100 µg/ml). The mitochondria-targeting antioxidant MitoQ (2 μg/ml) was used as a positive control. Cell viability, ROS generation, RAGE, AGE-RAGE signaling pathway components, and inflammatory cytokine levels were analyzed. <i>F.</i> <i>ovata</i> and <i>A.</i> <i>vulgaris</i> extracts showed antioxidative effects in non-cell-based assays. Treatment of THP-1 cells with AGE significantly increased the protein levels of RAGE and significantly increased the mRNA expression of cytokines, including TNF-α, IL-1β, and IL-6. AGEs induced the generation of ROS and levels of signaling molecules downstream of RAGE, including phosphorylated and total Erk1/2, JNK, and p38 MAPK, although not significantly. <i>F.</i> <i>ovata</i> and <i>A.</i> <i>vulgaris</i> extracts significantly decreased the protein levels of RAGE and significantly decreased the mRNA levels of cytokines. In conclusion, this study revealed that <i>F.</i> <i>ovata</i> and <i>A.</i> <i>vulgaris</i> extracts exert anti-inflammatory effects through the AGE-RAGE axis. However, details on this anti-inflammatory effect through AGE-RAGE signaling should be further investigated.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s43188-021-00114-0.</p>\",\"PeriodicalId\":23181,\"journal\":{\"name\":\"Toxicological Research\",\"volume\":\"38 3\",\"pages\":\"331-343\"},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2022-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9247136/pdf/43188_2021_Article_114.pdf\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Toxicological Research\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1007/s43188-021-00114-0\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"TOXICOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Toxicological Research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s43188-021-00114-0","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"TOXICOLOGY","Score":null,"Total":0}
Fimbristylisovata and Artemisiavulgaris extracts inhibited AGE-mediated RAGE expression, ROS generation, and inflammation in THP-1 cells.
Advanced glycation end products (AGEs) can induce inflammatory signaling pathways through the receptor for AGEs (RAGE). Targeting RAGE could be a therapeutic strategy for treating chronic inflammation mediated by the AGE-RAGE axis. This study aimed to investigate the effects of Fimbristylisovata and Artemisiavulgaris extracts on AGE-RAGE signaling and AGE-mediated oxidative stress and inflammation in THP-1 cells. F.ovata and A.vulgaris were extracted by a Soxhlet extraction, and antioxidant capacity was evaluated using DPPH and ABTS assays. The human monocytic cell line THP-1 was treated with AGE (600 µg/ml) with and without F.ovata and A.vulgaris extracts (100 µg/ml). The mitochondria-targeting antioxidant MitoQ (2 μg/ml) was used as a positive control. Cell viability, ROS generation, RAGE, AGE-RAGE signaling pathway components, and inflammatory cytokine levels were analyzed. F.ovata and A.vulgaris extracts showed antioxidative effects in non-cell-based assays. Treatment of THP-1 cells with AGE significantly increased the protein levels of RAGE and significantly increased the mRNA expression of cytokines, including TNF-α, IL-1β, and IL-6. AGEs induced the generation of ROS and levels of signaling molecules downstream of RAGE, including phosphorylated and total Erk1/2, JNK, and p38 MAPK, although not significantly. F.ovata and A.vulgaris extracts significantly decreased the protein levels of RAGE and significantly decreased the mRNA levels of cytokines. In conclusion, this study revealed that F.ovata and A.vulgaris extracts exert anti-inflammatory effects through the AGE-RAGE axis. However, details on this anti-inflammatory effect through AGE-RAGE signaling should be further investigated.
Supplementary information: The online version contains supplementary material available at 10.1007/s43188-021-00114-0.
期刊介绍:
Toxicological Research is the official journal of the Korean Society of Toxicology. The journal covers all areas of Toxicological Research of chemicals, drugs and environmental agents affecting human and animals, which in turn impact public health. The journal’s mission is to disseminate scientific and technical information on diverse areas of toxicological research. Contributions by toxicologists, molecular biologists, geneticists, biochemists, pharmacologists, clinical researchers and epidemiologists with a global view on public health through toxicological research are welcome. Emphasis will be given to articles providing an understanding of the toxicological mechanisms affecting animal, human and public health. In the case of research articles using natural extracts, detailed information with respect to the origin, extraction method, chemical profiles, and characterization of standard compounds to ensure the reproducible pharmacological activity should be provided.