捕获线虫真菌基因操作的工具和基本程序。

IF 4.6 2区 生物学 Q1 MYCOLOGY Mycology Pub Date : 2023-01-01 DOI:10.1080/21501203.2023.2165186
Shunxian Wang, Xingzhong Liu
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引用次数: 3

摘要

捕获线虫真菌(nematotetrapping fungi, NTF)是通过菌丝衍生的多种复杂的捕获器官捕获线虫的大多数肉食性微生物。除了腐生外,它们还可以采取食肉的生活方式,从线虫那里获取额外的营养。NTF作为一种特殊的真菌类群,不仅是研究真菌生活方式转变的良好模式生物,也是探索线虫生物防治的天然资源。然而,NTF的食肉机制仍然知之甚少。目前,NTF的组学研究已经提供了许多与肉食性性状表型相关的基因和途径,这些基因和途径需要分子工具来验证。本文综述了NTF基因操作工具的发展和进展,包括转化的方法和策略、随机基因诱变方法和靶基因诱变方法。总结和讨论了每种方法的原理和实用方法,并描述了每种工具的基本操作流程。本文为NTF及其他类群真菌的研究提供了明确的参考和指导。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Tools and basic procedures of gene manipulation in nematode-trapping fungi.

Nematode-trapping fungi (NTF) are the majority of carnivorous microbes to capture nematodes through diverse and sophisticated trapping organs derived from hyphae. They can adopt carnivorous lifestyles in addition to saprophytism to obtain extra-nutrition from nematodes. As a special group of fungi, the NTF are not only excellent model organism for studying lifestyle transition of fungi but also natural resources of exploring biological control of nematodes. However, the carnivorous mechanism of NTF remains poorly understood. Nowadays, the omics studies of NTF have provided numerous genes and pathways that are associated with the phenotypes of carnivorous traits, which need molecular tools to verify. Here, we review the development and progress of gene manipulation tools in NTF, including methodology and strategy of transformation, random gene mutagenesis methods and target gene mutagenesis methods. The principle and practical approach for each method was summarized and discussed, and the basic operational flow for each tool was described. This paper offers a clear reference and instruction for researchers who work on NTF as well as other group of fungi.

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来源期刊
Mycology
Mycology Medicine-Infectious Diseases
CiteScore
9.10
自引率
0.00%
发文量
18
审稿时长
13 weeks
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