COVID-19患者的氧化应激、DNA损伤和炎症

IF 0.9 Q3 MEDICINE, GENERAL & INTERNAL Northern Clinics of Istanbul Pub Date : 2023-01-01 DOI:10.14744/nci.2022.00947
Guven Bektemur, Kubra Bozali, Sahin Colak, Selman Aktas, Eray Metin Guler
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引用次数: 1

摘要

目的:COVID-19患者严重的炎症和氧化应激可引起累积的抗病毒作用,严重的炎症可增加组织、氧化损伤和DNA损伤。因此,本研究对COVID-19患者的氧化应激、DNA损伤和炎症生物标志物进行了研究。方法:本研究采集了150例经聚合酶链反应诊断的Covid-19患者和150例具有相同人口统计学特征的健康志愿者的血液样本。用光度法测定总氧化状态(TOS)、总抗氧化状态(TAS)、总硫醇(TT)、天然硫醇和髓过氧化物酶(MPO)活性。采用商用试剂盒,采用ELISA法检测炎症标志物肿瘤坏死因子-α (TNF-α)、白细胞介素1β (IL-1β)、白细胞介素6 (IL-6)水平。采用彗星试验评价基因毒性作用。结果:氧化应激生物标志物;二硫化物、TOS、MPO、氧化应激指数以及炎症生物标志物IL-1β、IL-6、TNF-α水平和DNA损伤均升高(结论:在COVID-19患者中,诱导的DNA损伤、炎症和氧化应激可指导疾病的预后和治疗策略。
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Oxidative stress, DNA damage, and inflammation in COVID-19 patients.
OBJECTIVE Severe inflammation and oxidative stress seen in COVID-19 patients cause cumulative antiviral effects, and serious inflammation increases tissue, oxidative damage, and DNA damage. Therefore, in this study, oxidative stress, DNA damage, and inflammation biomarkers were investigated in patients diagnosed with COVID-19. METHODS In this study, blood samples were obtained from 150 Covid-19 patients diagnosed by polymerase chain reaction and 150 healthy volunteers with the same demographic characteristics. Total oxidant status (TOS), total antioxidant status (TAS), total thiol (TT), native thiol, and myeloperoxidase (MPO) activities were measured by photometric methods. The levels of tumor necrosis factor-alpha (TNF-α), interleukin 1 beta (IL-1β), and interleukin 6 (IL-6), which are inflammation markers, were measured by the ELISA method using commercial kits. The genotoxic effect was evaluated by Comet Assay. RESULTS The oxidative stress biomarkers; Disulfide, TOS, MPO, oxidative stress index, and IL-1β, IL-6, and TNF-α levels of inflammation biomarkers and the DNA damage in COVID-19 patients were increased (p<0.001), and the levels of TAS, TT, and NT In COVID-19 patients were decreased (p<0.001). CONCLUSION In COVID-19 patients, induced DNA damage, inflammation, and oxidative stress can guide the prognosis and treatment strategies of the disease.
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来源期刊
Northern Clinics of Istanbul
Northern Clinics of Istanbul MEDICINE, GENERAL & INTERNAL-
CiteScore
0.40
自引率
0.00%
发文量
48
审稿时长
10 weeks
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