Sarah Currò, Luca Fasolato, Stefania Balzan, Giacomo Biziato, Francesco Paesanti, Luca Bargelloni, Barbara Cardazzo, Enrico Novelli
{"title":"用阻抗法评价双壳类软体动物的大肠杆菌污染:与最可能数分析的比较及其与环境参数的相关性。","authors":"Sarah Currò, Luca Fasolato, Stefania Balzan, Giacomo Biziato, Francesco Paesanti, Luca Bargelloni, Barbara Cardazzo, Enrico Novelli","doi":"10.4081/ijfs.2023.11103","DOIUrl":null,"url":null,"abstract":"<p><p>The application of an electrochemical (impedance) tool for monitoring <i>Escherichia coli</i> contamination in shellfish was evaluated after 13 months of observation. The primary aim of the present study was to compare the standard most probable number (MPN) and μ-trac 4200 (log imped/100 g) for the assessment of <i>E. coli</i> contamination (log MPN/100 g) in non-depurated bivalve mollusks (BM) from five sampling areas of the Veneto-Emilian coast (Italy) (118 samples). The secondary aim was to evaluate the correlation between <i>E. coli</i> concentrations in BM and environmental factors on a large data set (690). The methods showed a moderate, positive correlation (0.60 and 0.69 Pearson and Spearman coefficients, respectively; P<i><</i>0.01) in <i>Ruditapes philippinarum.</i> The McNemar test indicated analogous sample classification between methods, and the impedance method overestimated the most contaminated class (P=0.03; >4,600 MPN/100 g). The results highlighted the suitability of the impedance method for a faster evaluation and routine use especially in clams, while in <i>Mytilus</i> it seemed less effective. Different models built by multivariate permutational variance analysis and multinomial logistic regression selected the suitable environmental features able to predict the <i>E. coli</i> load. Overall, salinity and season affected the <i>E. coli</i> contamination, whereas locally it was mainly influenced by hydrometry and salinity. The application of the impedance method coupled with environmental data analysis could help purification phase management to adhere to legal limits and could represent an advantage for local control authorities to define actions, considering extreme meteorological events' effects as a proactive reaction to climate change.</p>","PeriodicalId":14508,"journal":{"name":"Italian Journal of Food Safety","volume":null,"pages":null},"PeriodicalIF":1.8000,"publicationDate":"2023-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/bb/63/ijfs-12-2-11103.PMC10316231.pdf","citationCount":"0","resultStr":"{\"title\":\"Evaluating <i>Escherichia coli</i> contamination in bivalve mollusks using the impedance method: a comparison with most probable number analyses and correlation with environmental parameters.\",\"authors\":\"Sarah Currò, Luca Fasolato, Stefania Balzan, Giacomo Biziato, Francesco Paesanti, Luca Bargelloni, Barbara Cardazzo, Enrico Novelli\",\"doi\":\"10.4081/ijfs.2023.11103\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The application of an electrochemical (impedance) tool for monitoring <i>Escherichia coli</i> contamination in shellfish was evaluated after 13 months of observation. The primary aim of the present study was to compare the standard most probable number (MPN) and μ-trac 4200 (log imped/100 g) for the assessment of <i>E. coli</i> contamination (log MPN/100 g) in non-depurated bivalve mollusks (BM) from five sampling areas of the Veneto-Emilian coast (Italy) (118 samples). The secondary aim was to evaluate the correlation between <i>E. coli</i> concentrations in BM and environmental factors on a large data set (690). The methods showed a moderate, positive correlation (0.60 and 0.69 Pearson and Spearman coefficients, respectively; P<i><</i>0.01) in <i>Ruditapes philippinarum.</i> The McNemar test indicated analogous sample classification between methods, and the impedance method overestimated the most contaminated class (P=0.03; >4,600 MPN/100 g). The results highlighted the suitability of the impedance method for a faster evaluation and routine use especially in clams, while in <i>Mytilus</i> it seemed less effective. Different models built by multivariate permutational variance analysis and multinomial logistic regression selected the suitable environmental features able to predict the <i>E. coli</i> load. Overall, salinity and season affected the <i>E. coli</i> contamination, whereas locally it was mainly influenced by hydrometry and salinity. 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Evaluating Escherichia coli contamination in bivalve mollusks using the impedance method: a comparison with most probable number analyses and correlation with environmental parameters.
The application of an electrochemical (impedance) tool for monitoring Escherichia coli contamination in shellfish was evaluated after 13 months of observation. The primary aim of the present study was to compare the standard most probable number (MPN) and μ-trac 4200 (log imped/100 g) for the assessment of E. coli contamination (log MPN/100 g) in non-depurated bivalve mollusks (BM) from five sampling areas of the Veneto-Emilian coast (Italy) (118 samples). The secondary aim was to evaluate the correlation between E. coli concentrations in BM and environmental factors on a large data set (690). The methods showed a moderate, positive correlation (0.60 and 0.69 Pearson and Spearman coefficients, respectively; P<0.01) in Ruditapes philippinarum. The McNemar test indicated analogous sample classification between methods, and the impedance method overestimated the most contaminated class (P=0.03; >4,600 MPN/100 g). The results highlighted the suitability of the impedance method for a faster evaluation and routine use especially in clams, while in Mytilus it seemed less effective. Different models built by multivariate permutational variance analysis and multinomial logistic regression selected the suitable environmental features able to predict the E. coli load. Overall, salinity and season affected the E. coli contamination, whereas locally it was mainly influenced by hydrometry and salinity. The application of the impedance method coupled with environmental data analysis could help purification phase management to adhere to legal limits and could represent an advantage for local control authorities to define actions, considering extreme meteorological events' effects as a proactive reaction to climate change.
期刊介绍:
The Journal of Food Safety (IJFS) is the official journal of the Italian Association of Veterinary Food Hygienists (AIVI). The Journal addresses veterinary food hygienists, specialists in the food industry and experts offering technical support and advice on food of animal origin. The Journal of Food Safety publishes original research papers concerning food safety and hygiene, animal health, zoonoses and food safety, food safety economics. Reviews, editorials, technical reports, brief notes, conference proceedings, letters to the Editor, book reviews are also welcome. Every article published in the Journal will be peer-reviewed by experts in the field and selected by members of the editorial board. The publication of manuscripts is subject to the approval of the Editor who has knowledge of the field discussed in the manuscript in accordance with the principles of Peer Review; referees will be selected from the Editorial Board or among qualified scientists of the international scientific community. Articles must be written in English and must adhere to the guidelines and details contained in the Instructions to Authors.