基于细菌外膜囊泡的肿瘤疫苗 mRNA 运送平台。

Xiaoyu Gao, Yao Li, Guangjun Nie, Xiao Zhao
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摘要

定制化肿瘤 mRNA 疫苗的快速展示和递送方法十分有限。本文通过对 RNA 结合蛋白 L7Ae 进行表面工程,将细菌衍生的外膜囊泡 (OMV) 用作 mRNA 递送平台。 OMV-L7Ae 可通过 L7Ae-boxC/D 结合快速吸附盒 C/D 序列标记的 mRNA 抗原,并将其递送至 HEK-293T 细胞和树突状细胞。该平台提供了一种有别于脂质纳米颗粒(LNPs)的 mRNA 递送技术,可用于个性化 mRNA 肿瘤疫苗接种,并采用即插即用策略,适合快速制备针对不同肿瘤抗原的个性化 mRNA 肿瘤疫苗。主要特点 OMV 通过 L7Ae-boxC/D 结合被用作 mRNA 递送平台。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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mRNA Delivery Platform Based on Bacterial Outer Membrane Vesicles for Tumor Vaccine.

The rapid display and delivery method for customized tumor mRNA vaccines is limited. Herein, bacteria-derived outer membrane vesicles (OMVs) are employed as an mRNA delivery platform by surface engineering of an RNA-binding protein, L7Ae. OMV-L7Ae can rapidly adsorb boxC/D sequence-labeled mRNA antigens through L7Ae-boxC/D binding and deliver them into HEK-293T and dendritic cells. This platform provides an mRNA delivery technology distinct from lipid nanoparticles (LNPs) for personalized mRNA tumor vaccination and with a Plug-and-Display strategy suitable for rapid preparation of the personalized mRNA tumor vaccine against varied tumor antigens. Key features OMVs are employed as an mRNA delivery platform through L7Ae-boxC/D binding.

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