离线体外循环光子疗法中单核细胞的收集效率:处理时间能否缩短?

IF 2.4 3区 医学 Q2 HEMATOLOGY Blood Transfusion Pub Date : 2024-03-01 Epub Date: 2023-07-14 DOI:10.2450/BloodTransfus.442
Orkan Kartal, Nadja Lindlbauer, Sandra Laner-Plamberger, Eva Rohde, Fabian Foettinger, Laura Ombres, Georg Zimmermann, Cornelia Mrazek, Wanda Lauth, Christoph Grabmer
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引用次数: 0

摘要

背景:体外射血疗法(ECP)是一种行之有效的细胞疗法,但治疗各种疾病(如皮肤T细胞淋巴瘤、移植物抗宿主病和移植后器官排斥反应)耗时长、负担重。至于需要收集多少单核细胞(MNC)才能获得对 ECP 的临床反应,目前仍有争议。这项回顾性研究的目的是通过流式细胞术确定单核细胞产物(MCP)中淋巴细胞、单核细胞和中性粒细胞的数量,以及离线 ECP 环境下的收集效率:我们收集了使用 Spectra Optia 设备进行 162 次 ECP 程序收集 MNC 的 10 位不同患者的数据。使用血液分析仪测定 MCP 的白细胞(WBC)计数。用流式细胞术分析 MNC 的 CD45 和 CD14 表达,以准确确定收集的淋巴细胞和单核细胞部分:结果:收集的 MCP 显示细胞产量高,MNC 为 55.3×106/kg,淋巴细胞为 41.1×106/kg。MCP 的特点是 MNC 比例高(81.3%),中性粒细胞比例低(18.7%)。白细胞和 MNC 的平均收集效率分别为 23.9% 和 62.0%。MNC 部分显示患者外周血细胞计数与 MCP 计数之间存在中度至高度相关性:本研究是通过流式细胞术测定 ECP 的 MCP 中单核细胞与淋巴细胞关系的少数报告之一。与在线 ECP 的历史数据相比,离线 ECP 处理一个总血量的细胞产量要高得多。因此,为了减轻患者的负担,我们建议大幅缩短离线 ECP 处理时间。
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Collection efficiency of mononuclear cells in offline extracorporeal photopheresis: can processing time be shortened?

Background: Extracorporeal photopheresis (ECP) is a well-established but lengthy and burdensome cell-based therapy for various diseases such as cutaneous T-cell lymphoma, graft-versus-host disease and organ rejection after transplantation. The number of mononuclear cells (MNCs) that needs to be collected to obtain a clinical response to ECP is still under debate. The purpose of this retrospective study was to determine the number of lymphocytes, monocytes and neutrophils in mononuclear cell products (MCP) by flow cytometry and the collection efficiency in the offline ECP setting.

Materials and methods: We collected data from 10 different patients undergoing 162 ECP procedures using the Spectra Optia device for MNC collection. White blood cell (WBC) count of MCP was determined using a hematology analyzer. MNCs were analyzed for CD45 and CD14 expression by flow cytometry to exactly determine the collected lymphocyte and monocyte fractions.

Results: Collected MCP showed high cell yields with 55.3×106/kg MNCs and 41.1×106/kg lymphocytes. MCP were characterized by high MNC (81.3%) and low neutrophils (18.7%) percentage. Mean collection efficiency for WBCs and for MNCs was 23.9% and 62.0%, respectively. The MNC fraction showed a moderate to high correlation between peripheral blood cell count of patients and MCP count.

Discussion: This study is one of a few reports showing the monocyte-to-lymphocyte relation in MCP for ECP determined by flow cytometry. In comparison to historical data from inline ECP, the offline ECP processing one total blood volume results in considerably higher cell yields. For this reason, and to reduce the burden on patients, we propose that the offline ECP processing time can be substantially reduced.

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来源期刊
Blood Transfusion
Blood Transfusion HEMATOLOGY-
CiteScore
6.10
自引率
2.70%
发文量
91
审稿时长
2 months
期刊介绍: Blood Transfusion welcomes international submissions of Original Articles, Review Articles, Case Reports and Letters on all the fields related to Transfusion Medicine.
期刊最新文献
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