Abdel-Fattah M. El-Sayed, Claudia Figueiredo-Silva, Salma M. S. Zeid, Sarah O. Makled
{"title":"金属-氨基酸复合物(锌、硒、铜、铁和锰)可增强尼罗罗非鱼的免疫反应、抗氧化能力、肝功能酶和细胞因子基因的表达。","authors":"Abdel-Fattah M. El-Sayed, Claudia Figueiredo-Silva, Salma M. S. Zeid, Sarah O. Makled","doi":"10.1002/aah.10194","DOIUrl":null,"url":null,"abstract":"<p>\n \n </p>","PeriodicalId":15235,"journal":{"name":"Journal of aquatic animal health","volume":"35 4","pages":"248-262"},"PeriodicalIF":1.5000,"publicationDate":"2023-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Metal–amino acid complexes (Zn, Se, Cu, Fe, and Mn) enhance immune response, antioxidant capacity, liver function enzymes, and expression of cytokine genes in Nile Tilapia reared under field conditions\",\"authors\":\"Abdel-Fattah M. El-Sayed, Claudia Figueiredo-Silva, Salma M. S. Zeid, Sarah O. Makled\",\"doi\":\"10.1002/aah.10194\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>\\n \\n </p>\",\"PeriodicalId\":15235,\"journal\":{\"name\":\"Journal of aquatic animal health\",\"volume\":\"35 4\",\"pages\":\"248-262\"},\"PeriodicalIF\":1.5000,\"publicationDate\":\"2023-07-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of aquatic animal health\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/aah.10194\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"FISHERIES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of aquatic animal health","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/aah.10194","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"FISHERIES","Score":null,"Total":0}
引用次数: 0
摘要
目的:研究了在野外条件下，用金属-氨基酸复合物(MAACs)部分或全部替代饲料中无机微量物质(ITM)对尼罗罗非鱼(Oreochromis niloticus)免疫应答、抗氧化能力、细胞因子基因表达和整体健康状况的影响。方法:将平均初始体重约为90 g -1的全雄性尼罗罗非鱼分成四组，以每半区60尾鱼(30尾m-3)的密度放养。每个试验区对养殖条件和试验饲料进行1周的驯化。定期监测水质参数，包括温度(°C)、pH、溶解氧(mg L-1)和总氨(mg L-1)。maac的配方中含有与无机预混料中相同的矿物质浓度。金属-氨基酸复合物以0(对照)、25、50、75和100%的水平替代ITM预混料(表1)。将饲料饲喂笼养尼罗罗非鱼，每天两次，连续80天，每天饲喂量为其体重的3%。每10天称重一次，并重新调整每日口粮。在收获时，每个hapa的鱼被收集、计数和称重。每个hapa的5只鱼被快速麻醉，并用于化学和生理分析，包括抗氧化和免疫反应分析，肝功能分析和基因表达。结果:随着MAACs添加量的增加，溶菌酶、呼吸爆发酶(%)、替代补体酶(ACH50)、吞噬细胞、酚氧化酶、超氧化物歧化酶、谷胱甘肽过氧化物酶和肝功能酶的活性均有所提高，替代量达到50%。与对照组相比，添加maac的饲料显著上调了干扰素、肿瘤坏死因子、白细胞介素-1、血清碱性磷酸酶、综合微生物基因组、氯霉素乙酰转移酶和转化生长因子基因等细胞因子基因的表达。50% MAAC水平下，这些基因的表达量上调幅度最大，而随着饲粮MAAC水平的增加，这些基因的表达量下降到75%和100%。二次回归分析表明，在田间条件下，池内养殖尼罗罗非鱼的免疫和氧化应激反应、基因表达和整体健康状况的最大促进需要60-65%的MAAC。结论:用MAAC预混料替代ITM预混料可增强尼罗罗非鱼的免疫和抗氧化反应。
Metal–amino acid complexes (Zn, Se, Cu, Fe, and Mn) enhance immune response, antioxidant capacity, liver function enzymes, and expression of cytokine genes in Nile Tilapia reared under field conditions
期刊介绍：
The Journal of Aquatic Animal Health serves the international community of scientists and culturists concerned with the health of aquatic organisms. It carries research papers on the causes, effects, treatments, and prevention of diseases of marine and freshwater organisms, particularly fish and shellfish. In addition, it contains papers that describe biochemical and physiological investigations into fish health that relate to assessing the impacts of both environmental and pathogenic features.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
