Mohammad Naser Shafei, Omid Fakharzadeh Moghaddam, Vida Alikhani, Reza Mohebbati
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引用次数: 1
摘要
背景:桥脚被盖核(PPT)参与心血管调节。确定了PPT核中存在μ (μ)阿片受体。在本研究中，研究了该核在正常血压条件下的作用，以及这些受体在出血引起的低血压(HEM)中心血管功能的作用。方法:将动物分为各组:1组:对照组，2组:HEM, 3组:100 nmol吗啡(一般阿片受体激动剂)，4组:100 nmol纳洛酮(一般阿片受体拮抗剂)，5组:吗啡+ HEM, 6组:纳洛酮+ HEM。麻醉后插管两条股动脉，记录心血管参数及取血情况。诱导HEM 2分钟后，向细胞核内注射药物，测量心血管参数。计算药物注射组和HEM组心血管反应的变化(Δ)，并与对照组和HEM组进行比较。结果:与对照组相比，HEM显著降低了收缩压和平均动脉压的变化，并增加了心率变化。微量注射吗啡使正常血压和HEM大鼠收缩压、平均动脉压和心率显著降低，纳洛酮使这些指标显著升高。结论:本研究表明，PPT核在HEM诱导的心血管反应中起调节作用。正常血压和HEM大鼠PPT核的µ阿片受体主要对血压和心率有抑制作用，纳洛酮微注射可消除这种作用。
The possible role of pedunculopontine tegmental nucleus (PPT) opioid receptors in the cardiovascular responses in normotensive and hemorrhagic hypotensive rats.
Background: The pedunculopontine tegmental nucleus (PPT) is involved in cardiovascular regulation. The presence of mu (μ) opioid receptors in the PPT nucleus has been determined. In the present study, the role of this nucleus in normotensive conditions and then the role of these receptors on cardiovascular function in hypotension induced by hemorrhage (HEM) were investigated.
Method: Animals were divided into the following groups: Group 1: control, Group 2: HEM, Group 3: morphine at dose 100 nmol (a general opioid receptor agonist), Group 4: naloxone at dose 100 nmol (a general opioid receptor antagonist), Group 5: morphine + HEM, and Group 6: naloxone + HEM. After anesthesia, two femoral arteries were cannulated to record the cardiovascular parameters and blood withdrawal. Two minutes after induction of HEM, drugs were injected into the nucleus, and cardiovascular parameters were measured. Changes (Δ) in cardiovascular responses due to drug injection and HEM were calculated and compared to control and HEM groups.
Results: HEM significantly reduced changes in systolic and mean arterial pressures and increased heart rate changes compared to control. Morphine microinjection in normotensive and HEM rats significantly decreased systolic blood pressure, mean arterial pressure, and heart rate, and naloxone significantly increased all these parameters.
Conclusion: This study showed that the PPT nucleus plays a role in modulating the cardiovascular responses induced by HEM. The µ opioid receptor of the PPT nucleus in the normotensive and HEM rats have inhibitory effects on blood pressure and heart rate mainly, and these effects are eliminated by naloxone microinjection.
期刊介绍：
Clinical and Experimental Hypertension is a reputable journal that has converted to a full Open Access format starting from Volume 45 in 2023. While previous volumes are still accessible through a Pay to Read model, the journal now provides free and open access to its content. It serves as an international platform for the exchange of up-to-date scientific and clinical information concerning both human and animal hypertension. The journal publishes a wide range of articles, including full research papers, solicited and unsolicited reviews, and commentaries. Through these publications, the journal aims to enhance current understanding and support the timely detection, management, control, and prevention of hypertension-related conditions.
One notable aspect of Clinical and Experimental Hypertension is its coverage of special issues that focus on the proceedings of symposia dedicated to hypertension research. This feature allows researchers and clinicians to delve deeper into the latest advancements in this field.
The journal is abstracted and indexed in several renowned databases, including Pharmacoeconomics and Outcomes News (Online), Reactions Weekly (Online), CABI, EBSCOhost, Elsevier BV, International Atomic Energy Agency, and the National Library of Medicine, among others. These affiliations ensure that the journal's content receives broad visibility and facilitates its discoverability by professionals and researchers in related disciplines.
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