含MA103佐剂的RBF蛋白诱导BALB/c小鼠对人呼吸道合胞病毒的保护性免疫

IF 1.3 4区 医学 Q4 INFECTIOUS DISEASES Japanese journal of infectious diseases Pub Date : 2023-05-24 DOI:10.7883/yoken.JJID.2022.476
Qiongqiong Fang, Hai Li, Hu Ren, Lei Cao, Hongqiao Hu, Yan Zhang, Wenbo Xu
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引用次数: 0

摘要

人类呼吸道合胞病毒(HRSV)疫苗的开发一直受到th2偏向性免疫反应导致的呼吸道疾病加剧的阻碍。本研究采用MA103和磷酸铝(aji - phos)佐剂对重组融合蛋白(大肠杆菌表达的F蛋白)的免疫原性进行了验证。两种佐剂均显著增加小鼠体内分泌干扰素γ (IFN-γ)的CD4+ T细胞的中和抗体滴度和数量。然而,基于免疫球蛋白G1 (IgG1)/IgG2a和IFN-γ/白细胞介素4分泌CD4+ T细胞的比例,MA103显著增强了th1偏向性免疫应答。RBF/MA103组肺病理损伤明显小于RBF/ jul - phos组。此外,RBF/MA103组肺中HRSV拷贝数减少了约3 × log10。这些结果表明MA103对小鼠HRSV具有更好的保护作用。
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RBF Protein with MA103 Adjuvant Elicited Protective Immunity against Human Respiratory Syncytial Virus in BALB/c Mice.

The development of a vaccine against human respiratory syncytial virus (HRSV) has been hampered by enhanced respiratory disease due to the Th2-biased immune response. In the present study, MA103 and aluminum phosphate (Adju-Phos) adjuvants were used to verify the immunogenicity of the recombinant fusion (RBF) protein (F protein expressed by Escherichia coli). Both adjuvants significantly increased the neutralizing antibody titer and number of interferon gamma (IFN-γ)-secreting CD4+ T cells in mice. Based on the immunoglobulin G1 (IgG1)/IgG2a and IFN-γ/interleukin 4-secreting CD4+ T cell ratio, however, MA103 significantly enhanced the Th1-biased immune response. The pathological damage to the lung in the RBF/MA103 group was less than what was seen in the RBF/Adju-Phos group. Additionally, the number of HRSV copies in the lungs of the RBF/MA103 group decreased by approximately 3 × log10. These results suggested that MA103 provides better protection against HRSV in mice.

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来源期刊
CiteScore
4.50
自引率
4.50%
发文量
172
审稿时长
2 months
期刊介绍: Japanese Journal of Infectious Diseases (JJID), an official bimonthly publication of National Institute of Infectious Diseases, Japan, publishes papers dealing with basic research on infectious diseases relevant to humans in the fields of bacteriology, virology, mycology, parasitology, medical entomology, vaccinology, and toxinology. Pathology, immunology, biochemistry, and blood safety related to microbial pathogens are among the fields covered. Sections include: original papers, short communications, epidemiological reports, methods, laboratory and epidemiology communications, letters to the editor, and reviews.
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