海马内cis-P tau显微注射诱导小鼠行为和突触可塑性的长期变化。

IF 4.7 2区 心理学 Q1 BEHAVIORAL SCIENCES Behavioral and Brain Functions Pub Date : 2023-05-25 DOI:10.1186/s12993-023-00211-0
Bakhtiarzadeh Fatemeh, Shahpasand Koorosh, Shojaei Amir, Fathollahi Yaghoub, Mirnajafi-Zadeh Javad
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引用次数: 0

摘要

背景:阿尔茨海默病伴随着异常高的顺式p tau积聚。然而,tau积累后行为的长期变化仍存在争议。本研究探讨了脑损伤对学习记忆、突触可塑性和海马细胞数量的长期影响。结果:将Cis-P tau微量注入C57BL/6小鼠海马背侧,制备阿尔茨海默病样模型。在y迷宫和Barnes迷宫测试中,注射Cis-P tau的动物表现出明显的学习和记忆障碍。在另一组动物中,在注射顺式p tau蛋白7个月后,在海马切片中评估长期增强(LTP)的产生。LTP诱导仅在海马背侧而非腹侧被破坏。海马背侧切片基底突触传递也减少。同时取海马标本,尼氏染色测定细胞数量。结果表明,与对照组相比,顺式P-tau注射动物海马背侧和腹侧的存活细胞数量明显减少。但海马背侧细胞数量的减少明显高于海马腹侧。结论:海马内注射cis-P tau在注射后7个月出现学习记忆障碍。这种损伤可能是由于LTP的破坏和海马背侧神经元数量的显著减少。
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Intra-hippocampal cis-P tau microinjection induces long-term changes in behavior and synaptic plasticity in mice.

Background: Alzheimer's disease is accompanied by an abnormal high accumulation of cis-P tau. However, the long-term changes in behavior following tau accumulation remains under debate. The present study investigated the long-term effects of tauopathy on learning and memory, synaptic plasticity, and hippocampal cell numbers.

Results: Cis-P tau was microinjected into the dorsal hippocampus to generate Alzheimer's like-disease model in C57BL/6 mice. Cis-P tau injected animals showed a significant impairment in learning and memory in Y-maze and Barnes maze tests. In another group of animals, the generation of long-term potentiation (LTP) was evaluated in hippocampal slices 7 months after cis-P tau injection. LTP induction was disrupted only in the dorsal but not ventral hippocampal slices. The basal synaptic transmission was also reduced in dorsal hippocampal slices. In addition, hippocampal sampling was done, and the number of cells was assessed by Nissl staining. Obtained results indicated that the number of survived cells was significantly reduced in the dorsal and ventral hippocampus of cis P-tau injected animals compared to the animals in control group. However, the decrement of cell number was higher in the dorsal compared to the ventral hippocampus.

Conclusions: In conclusion, intra-hippocampal cis-P tau injection produced learning and memory impairment at 7 months after its injection. This impairment might result from LTP disruption and a significant decrease in the number of neurons in the dorsal hippocampus.

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来源期刊
Behavioral and Brain Functions
Behavioral and Brain Functions 医学-行为科学
CiteScore
5.90
自引率
0.00%
发文量
11
审稿时长
6-12 weeks
期刊介绍: A well-established journal in the field of behavioral and cognitive neuroscience, Behavioral and Brain Functions welcomes manuscripts which provide insight into the neurobiological mechanisms underlying behavior and brain function, or dysfunction. The journal gives priority to manuscripts that combine both neurobiology and behavior in a non-clinical manner.
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