秀丽隐杆线虫卵母细胞转录沉默因素的表征。

IF 4.5 2区 生物学 Q1 Agricultural and Biological Sciences PLoS Genetics Pub Date : 2023-07-01 DOI:10.1371/journal.pgen.1010831
Mezmur D Belew, Emilie Chien, W Matthew Michael
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引用次数: 2

摘要

虽然几十年来人们已经认识到,前驱阻滞的卵母细胞在全球水平上是转录沉默的,但促进沉默的分子途径仍然难以捉摸。先前对秀丽隐杆线虫的研究表明,拓扑异构酶II (TOP-2)和凝聚素II与H3K9me异染色质途径协同作用,在L1饥饿期间沉默种系基因的表达,而pie1蛋白沉默了早期胚胎p谱系的基因组。在这里,我们发现所有这三种沉默系统,TOP-2/凝缩素II, H3K9me和PIE-1,都是卵母细胞转录抑制所必需的。我们发现沉默期间染色质上的H3K9me3标记显著增加,并且沉默是受细胞周期控制的。我们还发现PIE-1在沉默之前定位于核仁,而沉默期间核仁的溶解依赖于TOP-2/冷凝蛋白II。我们的数据确定了卵母细胞中基因组沉默的分子成分和触发因素,并建立了PIE-1核仁驻留与其抑制转录能力之间的联系。
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Characterization of factors that underlie transcriptional silencing in C. elegans oocytes.

While it has been appreciated for decades that prophase-arrested oocytes are transcriptionally silenced on a global level, the molecular pathways that promote silencing have remained elusive. Previous work in C. elegans has shown that both topoisomerase II (TOP-2) and condensin II collaborate with the H3K9me heterochromatin pathway to silence gene expression in the germline during L1 starvation, and that the PIE-1 protein silences the genome in the P-lineage of early embryos. Here, we show that all three of these silencing systems, TOP-2/condensin II, H3K9me, and PIE-1, are required for transcriptional repression in oocytes. We find that H3K9me3 marks increase dramatically on chromatin during silencing, and that silencing is under cell cycle control. We also find that PIE-1 localizes to the nucleolus just prior to silencing, and that nucleolar dissolution during silencing is dependent on TOP-2/condensin II. Our data identify both the molecular components and the trigger for genome silencing in oocytes and establish a link between PIE-1 nucleolar residency and its ability to repress transcription.

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来源期刊
PLoS Genetics
PLoS Genetics 生物-遗传学
CiteScore
8.10
自引率
2.20%
发文量
438
审稿时长
1 months
期刊介绍: PLOS Genetics is run by an international Editorial Board, headed by the Editors-in-Chief, Greg Barsh (HudsonAlpha Institute of Biotechnology, and Stanford University School of Medicine) and Greg Copenhaver (The University of North Carolina at Chapel Hill). Articles published in PLOS Genetics are archived in PubMed Central and cited in PubMed.
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