HPV E6诱导ADAM10表达升高对宫颈癌预后的影响

IF 1.1 4区 生物学 Q4 GENETICS & HEREDITY Genetic testing and molecular biomarkers Pub Date : 2023-05-01 DOI:10.1089/gtmb.2022.0170
Xuewang Guo, Yu Dou, Shuiqingqing Liu, Yue Du, Ruimeng Guo, Yingying Yue, Yu Xu, Xueying Liu, Yanying Xu
{"title":"HPV E6诱导ADAM10表达升高对宫颈癌预后的影响","authors":"Xuewang Guo,&nbsp;Yu Dou,&nbsp;Shuiqingqing Liu,&nbsp;Yue Du,&nbsp;Ruimeng Guo,&nbsp;Yingying Yue,&nbsp;Yu Xu,&nbsp;Xueying Liu,&nbsp;Yanying Xu","doi":"10.1089/gtmb.2022.0170","DOIUrl":null,"url":null,"abstract":"<p><p><b><i>Objective:</i></b> To explore the abnormal expression of <i>ADAM10</i>, its cause, and its clinical value in the prognosis of cervical lesions. <b><i>Methods:</i></b> The abnormal expression of <i>ADAM10</i> was explored using the Gene Expression Profiling Interactive Analysis database, and the abnormal expression in cervical lesions was verified using immunohistochemistry (IHC). The transfection effect of shRNA was evaluated using real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The expression of <i>ADAM10</i> in cells was analyzed using western blotting. <b><i>Results:</i></b> <i>ADAM10</i> was highly expressed in multiple cancers. As the disease progressed, the expression of <i>ADAM10</i> gradually increased (<i>p</i> < 0.05). Patients with higher expression of <i>ADAM10</i> had poorer survival outcomes than those with lower expression levels (<i>p</i> < 0.05). The expression levels of <i>ADAM10</i> decreased after expression levels of E6 was inhibited. <b><i>Conclusion:</i></b> <i>ADAM10</i> is highly expressed in cervical cancer; the higher the expression levels, the worse the survival outcome. HPV E6 is the critical driver of the elevated expression of <i>ADAM10</i> in cervical cancer.</p>","PeriodicalId":12603,"journal":{"name":"Genetic testing and molecular biomarkers","volume":"27 5","pages":"165-171"},"PeriodicalIF":1.1000,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Elevated Expression of <i>ADAM10</i> Induced by HPV E6 Influences the Prognosis of Cervical Cancer.\",\"authors\":\"Xuewang Guo,&nbsp;Yu Dou,&nbsp;Shuiqingqing Liu,&nbsp;Yue Du,&nbsp;Ruimeng Guo,&nbsp;Yingying Yue,&nbsp;Yu Xu,&nbsp;Xueying Liu,&nbsp;Yanying Xu\",\"doi\":\"10.1089/gtmb.2022.0170\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><b><i>Objective:</i></b> To explore the abnormal expression of <i>ADAM10</i>, its cause, and its clinical value in the prognosis of cervical lesions. <b><i>Methods:</i></b> The abnormal expression of <i>ADAM10</i> was explored using the Gene Expression Profiling Interactive Analysis database, and the abnormal expression in cervical lesions was verified using immunohistochemistry (IHC). The transfection effect of shRNA was evaluated using real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The expression of <i>ADAM10</i> in cells was analyzed using western blotting. <b><i>Results:</i></b> <i>ADAM10</i> was highly expressed in multiple cancers. As the disease progressed, the expression of <i>ADAM10</i> gradually increased (<i>p</i> < 0.05). Patients with higher expression of <i>ADAM10</i> had poorer survival outcomes than those with lower expression levels (<i>p</i> < 0.05). The expression levels of <i>ADAM10</i> decreased after expression levels of E6 was inhibited. <b><i>Conclusion:</i></b> <i>ADAM10</i> is highly expressed in cervical cancer; the higher the expression levels, the worse the survival outcome. HPV E6 is the critical driver of the elevated expression of <i>ADAM10</i> in cervical cancer.</p>\",\"PeriodicalId\":12603,\"journal\":{\"name\":\"Genetic testing and molecular biomarkers\",\"volume\":\"27 5\",\"pages\":\"165-171\"},\"PeriodicalIF\":1.1000,\"publicationDate\":\"2023-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Genetic testing and molecular biomarkers\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1089/gtmb.2022.0170\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"GENETICS & HEREDITY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Genetic testing and molecular biomarkers","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1089/gtmb.2022.0170","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
引用次数: 1

摘要

目的:探讨ADAM10在宫颈病变预后中的异常表达、原因及临床价值。方法:利用基因表达谱交互分析数据库探索ADAM10的异常表达,并利用免疫组织化学(IHC)验证宫颈病变中的异常表达。采用实时定量逆转录聚合酶链反应(qRT-PCR)评价shRNA转染效果。western blotting分析细胞中ADAM10的表达。结果:ADAM10在多种肿瘤中高表达。随着疾病的进展,ADAM10的表达逐渐增加(p ADAM10比表达水平较低的患者生存结果更差(p ADAM10在E6表达水平被抑制后下降)。结论:ADAM10在宫颈癌中高表达;表达水平越高,生存结果越差。HPV E6是宫颈癌中ADAM10表达升高的关键驱动因素。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Elevated Expression of ADAM10 Induced by HPV E6 Influences the Prognosis of Cervical Cancer.

Objective: To explore the abnormal expression of ADAM10, its cause, and its clinical value in the prognosis of cervical lesions. Methods: The abnormal expression of ADAM10 was explored using the Gene Expression Profiling Interactive Analysis database, and the abnormal expression in cervical lesions was verified using immunohistochemistry (IHC). The transfection effect of shRNA was evaluated using real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The expression of ADAM10 in cells was analyzed using western blotting. Results: ADAM10 was highly expressed in multiple cancers. As the disease progressed, the expression of ADAM10 gradually increased (p < 0.05). Patients with higher expression of ADAM10 had poorer survival outcomes than those with lower expression levels (p < 0.05). The expression levels of ADAM10 decreased after expression levels of E6 was inhibited. Conclusion: ADAM10 is highly expressed in cervical cancer; the higher the expression levels, the worse the survival outcome. HPV E6 is the critical driver of the elevated expression of ADAM10 in cervical cancer.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
2.50
自引率
7.10%
发文量
63
审稿时长
1 months
期刊介绍: Genetic Testing and Molecular Biomarkers is the leading peer-reviewed journal covering all aspects of human genetic testing including molecular biomarkers. The Journal provides a forum for the development of new technology; the application of testing to decision making in an increasingly varied set of clinical situations; ethical, legal, social, and economic aspects of genetic testing; and issues concerning effective genetic counseling. This is the definitive resource for researchers, clinicians, and scientists who develop, perform, and interpret genetic tests and their results. Genetic Testing and Molecular Biomarkers coverage includes: -Diagnosis across the life span- Risk assessment- Carrier detection in individuals, couples, and populations- Novel methods and new instrumentation for genetic testing- Results of molecular, biochemical, and cytogenetic testing- Genetic counseling
期刊最新文献
Association of ANRIL Gene Polymorphisms with Gastric Cancer Risk: A Case-Control Study. Immune Regulatory Circular RNAs, circRasGEF1B and circHIPK3, are Upregulated in Peripheral Blood Mononuclear Cells of COVID-19 Patients. Differential Analysis of Pathogenic Variants in Thoracic Aortic Aneurysm and Dissection at Different Ages. Evaluation of Multigene Methylation for Blood-Based Detection of Colorectal Cancer. Human Leukocyte Antigen-G Gene Polymorphism in Peninsular Malaysia: A Preliminary Report.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1