啮齿动物多囊肾模型中肾脏体积测量方法的准确性和处理时间:半自动肾脏分割的优越性。

IF 3.7 2区 医学 Q1 PHYSIOLOGY American Journal of Physiology-renal Physiology Pub Date : 2023-04-01 Epub Date: 2023-02-16 DOI:10.1152/ajprenal.00295.2022
Mary Claire Doss, Sean Mullen, Ronald Roye, Juling Zhou, Phillip Chumley, Elias Mrug, Darren P Wallace, Feng Qian, Peter C Harris, Bradley K Yoder, Harrison Kim, Michal Mrug
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引用次数: 0

摘要

使用磁共振成像(MRI)测量肾脏总体积(TKV)是监测常染色体显性多囊肾病(PKD)疾病进展的重要方法,在使用动物模型进行临床前研究时也越来越常见。人工勾画肾脏 MRI 区域轮廓(即人工方法 (MM))是确定 TKV 的一种传统方法,但非常耗时。我们开发了一种基于模板的半自动图像分割方法(SAM),并在三种常用的 PKD 模型中进行了验证:Cys1cpk/cpk小鼠、Pkd1RC/RC小鼠和Pkhd1pck/pck大鼠(每个模型n = 10)。我们将基于 SAM 的 TKV 与临床替代方法(包括使用三个肾脏维度的基于椭圆公式的方法 (EM)、最长肾脏长度法 (LM) 和被视为黄金标准的 MM)得出的 TKV 进行了比较。SAM和EM对Cys1cpk/cpk小鼠TKV评估的准确性都很高[类间相关系数(ICC)≥0.94]。在 Pkd1RC/RC 小鼠(ICC = 0.87、0.74)和 Pkhd1pck/pck 大鼠(ICC = 0.59、Cys1cpk/cpk 小鼠(3.6 ± 0.6 vs. 4.4 ± 0.7 min/kidney) 和 Pkd1RC/RC 小鼠 (3.1 ± 0.4 vs. 7.1 ± 2.6 min/kidney, 均 P < 0.001),但 Pkhd1PCK/PCK 大鼠 (3.7 ± 0.8 vs. 3.2 ± 0.5 min/kidney) 则不然。在所有研究模型中,LM 的速度最快(1 分钟),但与基于 MM 的 TKV 的相关性最差。Cys1cpk/cpk小鼠、Pkd1RC/RC小鼠和Pkhd1pck.pck大鼠的MM处理时间较长(66.1 ± 7.3、38.3 ± 7.5和29.2 ± 3.5分钟)。总之,SAM 是测定小鼠和大鼠 PKD 模型中 TKV 的一种快速而准确的方法。新进展和注意事项 肾脏总体积(TKV)是常染色体显性和常染色体隐性多囊肾疾病(ADPKD 和 ARPKD)临床前研究中的一种重要读数。由于传统的 TKV 评估方法是在所有图像中手动勾画肾脏区域轮廓,非常耗时,因此我们开发了一种基于模板的半自动图像分割方法(SAM),并在三种常用的 ADPKD 和 ARPKD 模型中进行了验证。基于 SAM 的 TKV 测量在小鼠和大鼠 ARPKD 和 ADPKD 模型中快速、重复性高且准确。
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Accuracy and processing time of kidney volume measurement methods in rodents polycystic kidney disease models: superiority of semiautomated kidney segmentation.

Measurement of total kidney volume (TKV) using magnetic resonance imaging (MRI) is a valuable approach for monitoring disease progression in autosomal dominant polycystic kidney disease (PKD) and is becoming more common in preclinical studies using animal models. Manual contouring of kidney MRI areas [i.e., manual method (MM)] is a conventional, but time-consuming, way to determine TKV. We developed a template-based semiautomatic image segmentation method (SAM) and validated it in three commonly used PKD models: Cys1cpk/cpk mice, Pkd1RC/RC mice, and Pkhd1pck/pck rats (n = 10 per model). We compared SAM-based TKV with that obtained by clinical alternatives including the ellipsoid formula-based method (EM) using three kidney dimensions, the longest kidney length method (LM), and MM, which is considered the gold standard. Both SAM and EM presented high accuracy in TKV assessment in Cys1cpk/cpk mice [interclass correlation coefficient (ICC) ≥ 0.94]. SAM was superior to EM and LM in Pkd1RC/RC mice (ICC = 0.87, 0.74, and <0.10 for SAM, EM, and LM, respectively) and Pkhd1pck/pck rats (ICC = 0.59, <0.10, and <0.10, respectively). Also, SAM outperformed EM in processing time in Cys1cpk/cpk mice (3.6 ± 0.6 vs. 4.4 ± 0.7 min/kidney) and Pkd1RC/RC mice (3.1 ± 0.4 vs. 7.1 ± 2.6 min/kidney, both P < 0.001) but not in Pkhd1PCK/PCK rats (3.7 ± 0.8 vs. 3.2 ± 0.5 min/kidney). LM was the fastest (∼1 min) but correlated most poorly with MM-based TKV in all studied models. Processing times by MM were longer for Cys1cpk/cpk mice, Pkd1RC/RC mice, and Pkhd1pck.pck rats (66.1 ± 7.3, 38.3 ± 7.5, and 29.2 ± 3.5 min). In summary, SAM is a fast and accurate method to determine TKV in mouse and rat PKD models.NEW & NOTEWORTHY Total kidney volume (TKV) is a valuable readout in preclinical studies for autosomal dominant and autosomal recessive polycystic kidney diseases (ADPKD and ARPKD). Since conventional TKV assessment by manual contouring of kidney areas in all images is time-consuming, we developed a template-based semiautomatic image segmentation method (SAM) and validated it in three commonly used ADPKD and ARPKD models. SAM-based TKV measurements were fast, highly reproducible, and accurate across mouse and rat ARPKD and ADPKD models.

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来源期刊
CiteScore
8.40
自引率
7.10%
发文量
154
审稿时长
2-4 weeks
期刊介绍: The American Journal of Physiology - Renal Physiology publishes original manuscripts on timely topics in both basic science and clinical research. Published articles address a broad range of subjects relating to the kidney and urinary tract, and may involve human or animal models, individual cell types, and isolated membrane systems. Also covered are the pathophysiological basis of renal disease processes, regulation of body fluids, and clinical research that provides mechanistic insights. Studies of renal function may be conducted using a wide range of approaches, such as biochemistry, immunology, genetics, mathematical modeling, molecular biology, as well as physiological and clinical methodologies.
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