类风湿关节炎患者外周血和滑膜液中 miR-124a 表达的诊断价值

IF 1.1 4区 生物学 Q4 GENETICS & HEREDITY Human Heredity Pub Date : 2023-01-01 Epub Date: 2023-06-14 DOI:10.1159/000529171
Tianhao Wu, Yanlong Zhang, Aqin Peng, Xirui Wu
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引用次数: 0

摘要

导言:类风湿性关节炎(RA)是一种慢性自身免疫性疾病,目前正严重威胁着人们的健康。以往的研究记录了各种 miRNA 在 RA 患者中的表达变化。本研究测定了 miR-124a 在 RA 患者中的表达,并估计了其对 RA 的诊断价值:采用反转录定量聚合酶链反应法测定外周血血浆、外周血单核细胞(PBMCs)和滑膜液中 miR-124a 的表达水平,然后进行皮尔逊相关分析。此外,还评估了 miR-124a 与类风湿因子(RF)、红细胞沉降率(ESR)和 28 个关节的疾病活动度评分(DAS28)等主要临床指标之间的关联。结果:miR-124a在RA患者中下调,miR-124a在血浆、PBMCs和滑膜液中的表达水平呈一定程度的正相关,miR-124a与RF、ESR和DAS28成反比。对于 RA 患者的诊断,血浆中 miR-124a 的 AUC 为 0.899,临界值为 0.800,灵敏度为 68.75%,特异度为 94.44%;PBMCs 中 miR-124a 的 AUC 为 0.937,临界值为 0.805,灵敏度为 82.50%,特异度为 91.67%;血浆联合 PBMCs 中 miR-124a 的 AUC 为 0.961,诊断价值高于独立血浆或PBMCs;滑膜液中miR-124a的AUC为0.929,临界值为0.835,敏感性为80.00%,特异性为88.89%。结论:miR-124a在RA患者的血浆、PBMCs和滑膜液中表达下调,对RA具有较高的诊断价值。
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The Diagnostic Value of miR-124a Expression in Peripheral Blood and Synovial Fluid of Patients with Rheumatoid Arthritis.

Introduction: Rheumatoid arthritis (RA), a chronic autoimmune disorder, is currently a severe health threat. Previous studies have documented the altered expression of various miRNAs in RA patients. This study determined the expression of miR-124a in RA patients and estimated its diagnostic value for RA.

Methods: A total of 80 RA patients were enrolled as the study subjects, and 36 patients with osteoarthritis were included, with another 36 healthy people as the controls. miR-124a expression levels in peripheral blood plasma, peripheral blood mononuclear cells (PBMCs), and synovial fluid were measured using reverse transcription quantitative polymerase chain reaction, followed by Pearson correlation analysis. Additionally, the association between miR-124a and major clinical indicators was assessed, such as rheumatoid factor (RF), erythrocyte sedimentation rate (ESR), and disease activity score of 28 joints (DAS28). The diagnostic efficacy of miR-124a expression in plasma, PBMCs, and synovial fluid for RA was evaluated by the receiver operating characteristic curve, and the difference in the area under the curve (AUC) was analyzed.

Results: miR-124a was downregulated in RA patients, and the expression levels of miR-124a in plasma, PBMCs, and synovial fluid showed a certain degree of positive correlation. miR-124a was inversely linked with RF, ESR, and DAS28. For the diagnosis of RA patients, the AUC of plasma miR-124a was 0.899 and the cut-off value was 0.800, with 68.75% sensitivity and 94.44% specificity; the AUC of miR-124a in PBMCs was 0.937 and the cut-off value was 0.805, with 82.50% sensitivity and 91.67% specificity; the AUC of miR-124a in plasma combined with PBMCs was 0.961, with a higher diagnostic value than independent plasma or PBMCs; the AUC of miR-124a in synovial fluid was 0.929 and the cut-off value was 0.835, with 80.00% sensitivity and 88.89% specificity.

Conclusion: miR-124a expression is downregulated in the plasma, PBMCs, and synovial fluid of RA patients and has a high diagnostic value for RA.

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来源期刊
Human Heredity
Human Heredity 生物-遗传学
CiteScore
2.50
自引率
0.00%
发文量
12
审稿时长
>12 weeks
期刊介绍: Gathering original research reports and short communications from all over the world, ''Human Heredity'' is devoted to methodological and applied research on the genetics of human populations, association and linkage analysis, genetic mechanisms of disease, and new methods for statistical genetics, for example, analysis of rare variants and results from next generation sequencing. The value of this information to many branches of medicine is shown by the number of citations the journal receives in fields ranging from immunology and hematology to epidemiology and public health planning, and the fact that at least 50% of all ''Human Heredity'' papers are still cited more than 8 years after publication (according to ISI Journal Citation Reports). Special issues on methodological topics (such as ‘Consanguinity and Genomics’ in 2014; ‘Analyzing Rare Variants in Complex Diseases’ in 2012) or reviews of advances in particular fields (‘Genetic Diversity in European Populations: Evolutionary Evidence and Medical Implications’ in 2014; ‘Genes and the Environment in Obesity’ in 2013) are published every year. Renowned experts in the field are invited to contribute to these special issues.
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