Rhea Michelle J Khodabux, Shanthi Mariappan, Uma Sekar
{"title":"某三级医疗中心临床分离金黄色葡萄球菌毒力因子谱及耐甲氧西林金黄色葡萄球菌SCCmec型流行情况","authors":"Rhea Michelle J Khodabux, Shanthi Mariappan, Uma Sekar","doi":"10.1055/s-0043-1764483","DOIUrl":null,"url":null,"abstract":"<p><p><b>Background</b> Methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) is a widely recognized multidrug-resistant bacteria presenting a major therapeutic challenge to clinicians. <i>Staphylococcus aureus</i> possesses a number of pathogenicity factors that attribute to the severity of infections. This study was undertaken to investigate the common virulence genes in clinical isolates of <i>Staphylococcus aureus</i> , determine their antimicrobial susceptibility profile, and to characterize the staphylococcal cassette chromosome mec (SCCmec) types among MRSA in a tertiary care center. <b>Materials and Methods</b> A total of 133 clinical isolates were included in this study. Susceptibility to various antibiotics was determined by disc diffusion method. Methicillin resistance was screened using cefoxitin disc; m <i>ecA</i> and <i>mecC</i> genes were detected using polymerase chain reaction (PCR). PCR was done to detect 12 virulence factors such as <i>hla</i> , <i>hlb</i> , <i>fnbA</i> , <i>fnbB</i> , <i>sea</i> , <i>seb</i> , <i>sec</i> , <i>icaA</i> , <i>clfA</i> , <i>tst</i> , <i>pvl,</i> and <i>eta</i> . SCCmec typing was done by multiplex PCR. <b>Results</b> Of the 133 clinical isolates, 54 (40.6%) were MRSA. The most common virulence gene detected was <i>hlb</i> (61.6%), <i>hla</i> (39%), and <i>fnbA</i> (37%). SCCmec type I was the most predominant. Mortality rate of 6.7% was observed among patients with staphylococcal infections. Univariate analysis of mortality associated virulence genes did not reveal any significant association between virulence genes and mortality. <b>Conclusion</b> The distribution of virulence genes is similar in both MRSA and methicillin-sensitive <i>Staphylococcus aureus</i> . MRSA belongs to the SCCmec types I to IV. Possession of multiple virulence factors and multidrug resistance profile makes <i>Staphylococcus aureus</i> a formidable pathogen in clinical settings.</p>","PeriodicalId":16149,"journal":{"name":"Journal of Laboratory Physicians","volume":"15 3","pages":"450-461"},"PeriodicalIF":0.9000,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e8/e7/10-1055-s-0043-1764483.PMC10411210.pdf","citationCount":"0","resultStr":"{\"title\":\"Spectrum of Virulence Factors in Clinical Isolates of <i>Staphylococcus aureus</i> and Prevalence of SCCmec Types in Methicillin-Resistant <i>Staphylococcus aureus</i> in a Tertiary Care Center.\",\"authors\":\"Rhea Michelle J Khodabux, Shanthi Mariappan, Uma Sekar\",\"doi\":\"10.1055/s-0043-1764483\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><b>Background</b> Methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) is a widely recognized multidrug-resistant bacteria presenting a major therapeutic challenge to clinicians. <i>Staphylococcus aureus</i> possesses a number of pathogenicity factors that attribute to the severity of infections. This study was undertaken to investigate the common virulence genes in clinical isolates of <i>Staphylococcus aureus</i> , determine their antimicrobial susceptibility profile, and to characterize the staphylococcal cassette chromosome mec (SCCmec) types among MRSA in a tertiary care center. <b>Materials and Methods</b> A total of 133 clinical isolates were included in this study. Susceptibility to various antibiotics was determined by disc diffusion method. Methicillin resistance was screened using cefoxitin disc; m <i>ecA</i> and <i>mecC</i> genes were detected using polymerase chain reaction (PCR). PCR was done to detect 12 virulence factors such as <i>hla</i> , <i>hlb</i> , <i>fnbA</i> , <i>fnbB</i> , <i>sea</i> , <i>seb</i> , <i>sec</i> , <i>icaA</i> , <i>clfA</i> , <i>tst</i> , <i>pvl,</i> and <i>eta</i> . SCCmec typing was done by multiplex PCR. <b>Results</b> Of the 133 clinical isolates, 54 (40.6%) were MRSA. The most common virulence gene detected was <i>hlb</i> (61.6%), <i>hla</i> (39%), and <i>fnbA</i> (37%). SCCmec type I was the most predominant. Mortality rate of 6.7% was observed among patients with staphylococcal infections. Univariate analysis of mortality associated virulence genes did not reveal any significant association between virulence genes and mortality. <b>Conclusion</b> The distribution of virulence genes is similar in both MRSA and methicillin-sensitive <i>Staphylococcus aureus</i> . MRSA belongs to the SCCmec types I to IV. Possession of multiple virulence factors and multidrug resistance profile makes <i>Staphylococcus aureus</i> a formidable pathogen in clinical settings.</p>\",\"PeriodicalId\":16149,\"journal\":{\"name\":\"Journal of Laboratory Physicians\",\"volume\":\"15 3\",\"pages\":\"450-461\"},\"PeriodicalIF\":0.9000,\"publicationDate\":\"2023-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e8/e7/10-1055-s-0043-1764483.PMC10411210.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Laboratory Physicians\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1055/s-0043-1764483\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"MEDICINE, GENERAL & INTERNAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Laboratory Physicians","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1055/s-0043-1764483","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MEDICINE, GENERAL & INTERNAL","Score":null,"Total":0}
Spectrum of Virulence Factors in Clinical Isolates of Staphylococcus aureus and Prevalence of SCCmec Types in Methicillin-Resistant Staphylococcus aureus in a Tertiary Care Center.
Background Methicillin-resistant Staphylococcus aureus (MRSA) is a widely recognized multidrug-resistant bacteria presenting a major therapeutic challenge to clinicians. Staphylococcus aureus possesses a number of pathogenicity factors that attribute to the severity of infections. This study was undertaken to investigate the common virulence genes in clinical isolates of Staphylococcus aureus , determine their antimicrobial susceptibility profile, and to characterize the staphylococcal cassette chromosome mec (SCCmec) types among MRSA in a tertiary care center. Materials and Methods A total of 133 clinical isolates were included in this study. Susceptibility to various antibiotics was determined by disc diffusion method. Methicillin resistance was screened using cefoxitin disc; m ecA and mecC genes were detected using polymerase chain reaction (PCR). PCR was done to detect 12 virulence factors such as hla , hlb , fnbA , fnbB , sea , seb , sec , icaA , clfA , tst , pvl, and eta . SCCmec typing was done by multiplex PCR. Results Of the 133 clinical isolates, 54 (40.6%) were MRSA. The most common virulence gene detected was hlb (61.6%), hla (39%), and fnbA (37%). SCCmec type I was the most predominant. Mortality rate of 6.7% was observed among patients with staphylococcal infections. Univariate analysis of mortality associated virulence genes did not reveal any significant association between virulence genes and mortality. Conclusion The distribution of virulence genes is similar in both MRSA and methicillin-sensitive Staphylococcus aureus . MRSA belongs to the SCCmec types I to IV. Possession of multiple virulence factors and multidrug resistance profile makes Staphylococcus aureus a formidable pathogen in clinical settings.