某三级医疗中心临床分离金黄色葡萄球菌毒力因子谱及耐甲氧西林金黄色葡萄球菌SCCmec型流行情况

IF 0.9 Q3 MEDICINE, GENERAL & INTERNAL Journal of Laboratory Physicians Pub Date : 2023-09-01 DOI:10.1055/s-0043-1764483
Rhea Michelle J Khodabux, Shanthi Mariappan, Uma Sekar
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引用次数: 0

摘要

耐甲氧西林金黄色葡萄球菌(MRSA)是一种广泛认可的多重耐药细菌,对临床医生提出了重大的治疗挑战。金黄色葡萄球菌具有许多致病因素,这归因于感染的严重性。本研究旨在调查某三级医疗中心临床分离的金黄色葡萄球菌常见毒力基因,确定其抗微生物敏感性,并对MRSA中葡萄球菌盒染色体mec (SCCmec)类型进行表征。材料与方法本研究共收集133株临床分离株。采用圆盘扩散法测定对各种抗生素的药敏。采用头孢西丁片筛选甲氧西林耐药性;m采用聚合酶链反应(PCR)检测ecA和mecC基因。采用PCR检测hla、hlb、fnbA、fnbB、sea、seb、sec、icaA、clfA、test、pvl、eta等12种毒力因子。SCCmec分型采用多重PCR。结果133株临床分离物中,54株(40.6%)为MRSA;最常见的毒力基因为hlb(61.6%)、hla(39%)和fnbA(37%)。SCCmec I型最为显著。葡萄球菌感染患者的死亡率为6.7%。死亡率相关毒力基因的单变量分析未显示毒力基因与死亡率之间有任何显著关联。结论MRSA与甲氧西林敏感金黄色葡萄球菌毒力基因分布相似。MRSA属于SCCmec I型至IV型。金黄色葡萄球菌具有多重毒力因子和多重耐药特征,是临床中一种令人生畏的病原体。
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Spectrum of Virulence Factors in Clinical Isolates of Staphylococcus aureus and Prevalence of SCCmec Types in Methicillin-Resistant Staphylococcus aureus in a Tertiary Care Center.

Background  Methicillin-resistant Staphylococcus aureus (MRSA) is a widely recognized multidrug-resistant bacteria presenting a major therapeutic challenge to clinicians. Staphylococcus aureus possesses a number of pathogenicity factors that attribute to the severity of infections. This study was undertaken to investigate the common virulence genes in clinical isolates of Staphylococcus aureus , determine their antimicrobial susceptibility profile, and to characterize the staphylococcal cassette chromosome mec (SCCmec) types among MRSA in a tertiary care center. Materials and Methods  A total of 133 clinical isolates were included in this study. Susceptibility to various antibiotics was determined by disc diffusion method. Methicillin resistance was screened using cefoxitin disc; m ecA and mecC genes were detected using polymerase chain reaction (PCR). PCR was done to detect 12 virulence factors such as hla , hlb , fnbA , fnbB , sea , seb , sec , icaA , clfA , tst , pvl, and eta . SCCmec typing was done by multiplex PCR. Results  Of the 133 clinical isolates, 54 (40.6%) were MRSA. The most common virulence gene detected was hlb (61.6%), hla (39%), and fnbA (37%). SCCmec type I was the most predominant. Mortality rate of 6.7% was observed among patients with staphylococcal infections. Univariate analysis of mortality associated virulence genes did not reveal any significant association between virulence genes and mortality. Conclusion  The distribution of virulence genes is similar in both MRSA and methicillin-sensitive Staphylococcus aureus . MRSA belongs to the SCCmec types I to IV. Possession of multiple virulence factors and multidrug resistance profile makes Staphylococcus aureus a formidable pathogen in clinical settings.

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来源期刊
Journal of Laboratory Physicians
Journal of Laboratory Physicians MEDICINE, GENERAL & INTERNAL-
自引率
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发文量
99
审稿时长
31 weeks
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