多发性骨髓瘤的免疫表型:三级护理中心的经验。

IF 0.9 Q3 MEDICINE, GENERAL & INTERNAL Journal of Laboratory Physicians Pub Date : 2023-09-01 DOI:10.1055/s-0043-1761204
Asish Rath, Tribikram Panda, Jasmita Dass, Tulika Seth, Manoranjan Mahapatra, Seema Tyagi
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引用次数: 0

摘要

流式细胞术对浆细胞(PCs)的免疫分型和计数被认为具有重要的预后意义。然而,由于与形态学的差异和样品处理过程中pc的损失,流式细胞术计数具有挑战性。异常浆细胞(APCs)和正常浆细胞(NPCs)的计数和分化是困难的,因为在NPCs亚群中可以看到异常抗原表达。当依赖有限的抗体组时尤其如此。目的与目的应用流式细胞术研究新诊断的多发性骨髓瘤(MM)患者的免疫表型特征,评价单个抗原和联合抗原的敏感性和特异性。方法采用六色三管流式细胞仪检测新诊断MM患者(48例)和对照组(10例)的pc免疫表型。评估MM中抗原的敏感性和特异性,并与对照病例进行比较。结果大多数MM病例(n = 43)均存在抗原表达异常。包括CD81和CD38 MFI在内的多种标记物的组合应用于准确检测APC。
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Immunophenotypic Profile of Multiple Myeloma: A Tertiary Care Centre Experience.

Background  Immunophenotyping and enumeration of plasma cells (PCs) by flow cytometry are deemed to be prognostically significant. However, PCs enumeration by flow cytometry is challenging owing to discrepancy with morphology and PCs loss during sample processing. Enumeration and differentiation of abnormal plasma cells (APCs) and normal plasma cells (NPCs) is difficult because abnormal antigen expression can be seen in subsets of NPCs. This is particularly true when a limited panel of antibodies are relied upon. Aims and purpose  To study the immunophenotypic profile of newly diagnosed multiple myeloma (MM) cases by flow cytometry and evaluate the sensitivities and specificities of individual antigens and combinations. Methods  We studied immunophenotype of PCs in newly diagnosed MM cases ( n  = 48) and control cases ( n  = 10) by a 6-color, 3-tube flow cytometry panel. The sensitivities and specificities of antigens in MM were evaluated and compared with control cases. Results  Majority of MM cases ( n  = 43) had < 3% NPCs. CD19 was the most sensitive (100%) and CD81 was the most specific marker (100%) for differentiating APCs from NPCs. CD38 MFI came out as a useful marker for APCs identification. In combination, CD19 and CD81 had a higher sensitivity and specificity to detect APCs. Conclusion  NPCs may show aberrant antigen expression. A combination of multiple markers including CD81 and CD38 MFI should be used for accurate APC detection.

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来源期刊
Journal of Laboratory Physicians
Journal of Laboratory Physicians MEDICINE, GENERAL & INTERNAL-
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发文量
99
审稿时长
31 weeks
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