圆盘扩散法和琼脂稀释法检测皮肤和软组织感染葡萄球菌分离株莫匹罗星耐药性的比较。

IF 0.9 Q3 MEDICINE, GENERAL & INTERNAL Journal of Laboratory Physicians Pub Date : 2023-09-01 DOI:10.1055/s-0042-1760672
Vijaya Shivanna, Venkatesha Dasegowda
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引用次数: 2

摘要

目的和目的莫匹罗星是一种广泛使用的局部抗生素,用于治疗皮肤和软组织感染。这导致耐药性,导致治疗失败。因此,本研究旨在确定从皮肤和软组织感染中获得的葡萄球菌分离株中莫匹罗星耐药性的流行情况。比较了圆盘扩散法和琼脂稀释法检测莫匹罗星耐药性的效果。材料与方法本横断面研究于2018年1 - 12月在卡纳塔克邦一家三级卫生保健中心微生物科进行。临床样本,如伤口拭子、组织和脓液也包括在研究中。对所有葡萄球菌分离株分别使用5µg和200µg碟片进行低水平(MuL)和高水平(MuH)的莫匹罗星耐药筛选。用琼脂稀释法测定最低抑菌浓度(MIC)。结果100株葡萄球菌中,金黄色葡萄球菌68株,金黄色葡萄球菌32株,其中11株检出MuH。与金黄色葡萄球菌(1/11)相比,MuH在con(10/11)中更为常见。本研究未发现MuL。在我们的研究中,11株莫匹罗星耐药菌株中有10株对甲氧西林耐药,差异有统计学意义(p)结论在了解药敏报告后,司法处方莫匹罗星应成为标准做法。在资源有限的情况下,可通过盘片扩散筛查莫匹罗星耐药性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Comparison of Disk Diffusion and Agar Dilution Method for the Detection of Mupirocin Resistance in Staphylococcal Isolates from Skin and Soft Tissue Infections.

Aims and Objectives  Mupirocin is a widely used topical antibiotic for the treatment of skin and soft tissue infections. This has resulted in resistance leading to treatment failure. Hence, the present study aimed to determine the prevalence of mupirocin resistance among staphylococcal isolates obtained from the skin and soft tissue infections. Also, comparison of disc diffusion and agar dilution method in detecting mupirocin resistance was done. Materials and Methods  This cross-sectional study was conducted in the Department of Microbiology of a tertiary health care center in Karnataka from January to December, 2018. Clinical samples such as wound swabs, tissues, and pus were included in the study. All staphylococcal isolates were screened for mupirocin resistance using 5 µg and 200 µg discs for low-level (MuL) and high-level mupirocin resistance (MuH), respectively. Minimum inhibitory concentration (MIC) was determined using the agar dilution method. Results  Out of 100 staphylococcal isolates, 68 were Staphylococcus aureus and 32 were CoNS. MuH was detected in 11 isolates. MuH was more common in CoNS (10/11) compared with S. aureus (1/11). MuL was not found in the study. Discussion  In our study, 10 out of 11 mupirocin-resistant isolates were methicillin resistant, which is statistically significant ( p  < 0.05). The correlation between results of disc diffusion and MIC were appropriate in this study. Conclusion  Judicial prescription of mupirocin after knowing the susceptibility report should become the standard practice. Screening for mupirocin resistance can be done by disc diffusion in resource-limited settings.

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来源期刊
Journal of Laboratory Physicians
Journal of Laboratory Physicians MEDICINE, GENERAL & INTERNAL-
自引率
0.00%
发文量
99
审稿时长
31 weeks
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