用四种常用膨化剂冷冻公牛精液的精子特性及抗氧化剂和氧化剂水平的比较。

Pub Date : 2023-01-01 DOI:10.30466/vrf.2023.562594.3631
Kimia Maleki, Esmail Ayen, Amir Khaki, Ali Soleimanzadeh
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引用次数: 1

摘要

精子在新鲜精液中存活的时间很短,缓慢冷却至5.00˚C会杀死大量精子。本研究旨在比较4种膨化剂(手动膨化剂、三合甘油酯膨化剂、甾体甘油酯膨化剂和AndroMed膨化剂)的精液质量参数和抗氧化水平。精液样本取自西门塔尔牛种中心饲养的12头双用途西门塔尔公牛,该公牛使用人工阴道饲养了3个月。评估精子活力、活力、形态异常、质膜完整性、DNA损伤、染色质质量、总抗氧化能力(TAC)和脂质过氧化。用Triladyl冷冻的精液组的进行活力、活力、质膜完整性和TAC最高,丙二醛水平最低。三甘醇冻融后精液的运动参数高于其他组,与手动延长剂有显著差异。在所研究的延长剂中,三甘醇最适合用于西门塔尔公牛的精液冷冻。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Comparison of sperm characteristics and antioxidant and oxidant levels in bull semen frozen with four widely used extenders.

Sperm survives for a very short time in fresh semen, and slow cooling to 5.00 ˚C kills a large number of sperms. This study was aimed to compare the semen quality parameters and anti-oxidant levels in four extenders (manual, Triladyl, Steridyl and AndroMed). Semen samples were obtained from a total number of 12 dual-purpose Simmental bulls kept in the Simmental Cattle Breeding Center for a period of 3 months using an artificial vagina. Sperm viability, motility, abnormal morphology, plasma membrane integrity, DNA damage, chromatin quality, total antioxidant capacity (TAC) and lipid peroxidation were evaluated. The highest progressive motility, viability, plasma membrane integrity, and TAC and the lowest levels of malondialdehyde in the frozen-thawed semen belonged to the semen group frozen with Triladyl. Parameters of motility were higher in the frozen-thawed semen with Triladyl than in other groups, indicating a significant difference from the manual extender. Among the extenders studied, Triladyl was the most suitable for semen freezing in Simmental bulls.

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