对氧磷和草甘膦诱导DNA双链断裂，但不是II型拓扑异构酶毒素

IF 2.3 4区医学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Mutation research. Genetic toxicology and environmental mutagenesis Pub Date : 2023-08-01 DOI:10.1016/j.mrgentox.2023.503657

Regina Montero-Montoya , Karen Suárez-Larios, Luis Serrano-García

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引用次数: 0

摘要

我们检验了农药对氧磷和草甘膦通过毒害II型拓扑异构酶（topo-II）导致DNA双链断裂（DSB）的假设。G0期外周血淋巴细胞经杀虫剂处理，加或减TopoⅡ抑制剂ICRF-187后，可刺激其增殖；测定了诱导的细胞遗传学损伤。微核、染色质芽、核质桥和核外片段通过杀虫剂处理诱导，而不考虑ICRF-187的预处理。这些结果表明，这些杀虫剂不起拓扑Ⅱ型毒物的作用。DSB的诱导可能通过其他机制发生，例如对参与重组修复的其他蛋白质的影响。

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Paraoxon and glyphosate induce DNA double-strand breaks but are not type II topoisomerase poisons

We tested the hypothesis that the pesticides paraoxon and glyphosate cause DNA double-strand breaks (DSB) by poisoning the enzyme Type II topoisomerase (topo II). Peripheral lymphocytes in G0 phase, treated with the pesticides, plus or minus ICRF-187, an inhibitor of Topo II, were stimulated to proliferate; induced cytogenetic damage was measured.

Micronuclei, chromatin buds, nucleoplasmic bridges, and extranuclear fragments were induced by treatments with the pesticides, irrespective of the pre-treatment with ICRF-187. These results indicate that the pesticides do not act as topo II poisons. The induction of DSB may occur by other mechanisms, such as effects on other proteins involved in recombination repair.

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来源期刊

Mutation research. Genetic toxicology and environmental mutagenesis 生物-毒理学

CiteScore

3.80

自引率

5.30%

发文量

审稿时长

105 days

期刊介绍： Mutation Research - Genetic Toxicology and Environmental Mutagenesis (MRGTEM) publishes papers advancing knowledge in the field of genetic toxicology. Papers are welcomed in the following areas: New developments in genotoxicity testing of chemical agents (e.g. improvements in methodology of assay systems and interpretation of results). Alternatives to and refinement of the use of animals in genotoxicity testing. Nano-genotoxicology, the study of genotoxicity hazards and risks related to novel man-made nanomaterials. Studies of epigenetic changes in relation to genotoxic effects. The use of structure-activity relationships in predicting genotoxic effects. The isolation and chemical characterization of novel environmental mutagens. The measurement of genotoxic effects in human populations, when accompanied by quantitative measurements of environmental or occupational exposures. The application of novel technologies for assessing the hazard and risks associated with genotoxic substances (e.g. OMICS or other high-throughput approaches to genotoxicity testing). MRGTEM is now accepting submissions for a new section of the journal: Current Topics in Genotoxicity Testing, that will be dedicated to the discussion of current issues relating to design, interpretation and strategic use of genotoxicity tests. This section is envisaged to include discussions relating to the development of new international testing guidelines, but also to wider topics in the field. The evaluation of contrasting or opposing viewpoints is welcomed as long as the presentation is in accordance with the journal''s aims, scope, and policies.