Prp16与ADP复合物的晶体结构

IF 1.1 4区 生物学 Q4 BIOCHEMICAL RESEARCH METHODS Acta crystallographica. Section F, Structural biology communications Pub Date : 2023-08-07 DOI:10.1107/S2053230X23005721
Tim Benedict Garbers, Marieke Enders, Piotr Neumann, Ralf Ficner
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引用次数: 0

摘要

DEAH-box解旋酶在前信使核糖核酸剪接中起着至关重要的作用,因为它们负责剪接体的主要重排,并参与各种质量保证步骤。Prp16是剪接体催化过程中的驱动力,将C状态重塑为C*状态。本文报道了嗜热毛壳菌Prp16与ADP复合物的第一个晶体结构为1.9 Å分辨率。与其他剪接体DEAH盒解旋酶Prp2、Prp22和Prp43的比较揭示了完全相同的结构域结构。β-发夹是RecA2结构域的一个结构元件,表现出独特的位置,突出了其灵活性。对含有Prp16的剪接体复合物的冷冻电镜模型的分析表明,这些模型显示Prp16处于无核苷酸状态,使本文提出的模型成为Prp16与核苷酸复合物的第一个结构。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Crystal structure of Prp16 in complex with ADP

DEAH-box helicases play a crucial role in pre-mRNA splicing as they are responsible for major rearrangements of the spliceosome and are involved in various quality-ensuring steps. Prp16 is the driving force during spliceosomal catalysis, remodeling the C state into the C* state. Here, the first crystal structure of Prp16 from Chaetomium thermophilum in complex with ADP is reported at 1.9 Å resolution. Comparison with the other spliceosomal DEAH-box helicases Prp2, Prp22 and Prp43 reveals an overall identical domain architecture. The β-hairpin, which is a structural element of the RecA2 domain, exhibits a unique position, punctuating its flexibility. Analysis of cryo-EM models of spliceosomal complexes containing Prp16 reveals that these models show Prp16 in its nucleotide-free state, rendering the model presented here the first structure of Prp16 in complex with a nucleotide.

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来源期刊
Acta crystallographica. Section F, Structural biology communications
Acta crystallographica. Section F, Structural biology communications BIOCHEMICAL RESEARCH METHODSBIOCHEMISTRY &-BIOCHEMISTRY & MOLECULAR BIOLOGY
CiteScore
1.90
自引率
0.00%
发文量
95
期刊介绍: Acta Crystallographica Section F is a rapid structural biology communications journal. Articles on any aspect of structural biology, including structures determined using high-throughput methods or from iterative studies such as those used in the pharmaceutical industry, are welcomed by the journal. The journal offers the option of open access, and all communications benefit from unlimited free use of colour illustrations and no page charges. Authors are encouraged to submit multimedia content for publication with their articles. Acta Cryst. F has a dedicated online tool called publBio that is designed to make the preparation and submission of articles easier for authors.
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