Effect of nitrogen application levels on photosynthetic nitrogen distribution and use efficiency in soybean seedling leaves

IF 4 3区生物学 Q1 PLANT SCIENCES Journal of plant physiology Pub Date : 2023-08-01 DOI:10.1016/j.jplph.2023.154051

Binbin Qiang , Weixin Zhou , Xingjie Zhong , Chenye Fu , Liang Cao , Yuxian Zhang , Xijun Jin

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引用次数: 1

Abstract

Background

Nitrogen nutrition is strongly associated with crop growth and development. Nitrogen application level affects leaf size as well as nitrogen content and distribution, and thus affects photosynthetic nitrogen-use efficiency (PNUE) and yield. In this study, soybean varieties “Jinyuan 55” and “Keshan 1” were treated with nitrogen as urea at: N0, 0 kg hm⁻²; N0.5, 60 kg hm⁻²; N1, 120 kg hm⁻²; and N1.5, 180 kg hm⁻². We compared the effect of nitrogen level on plant morphology, biomass, photosynthetic physiology, nitrogen distribution, PNUE, and other soybean seedling leaf characteristics.

Results

Maximum carboxylation and electron transfer, net photosynthetic rates, and PNUE of both soybean varieties showed initial significant increases with increasing nitrogen application rate and subsequent stabilization. PNUE, carboxylation system components, electron transport components, and non-photosynthetic system distribution ratios in the photosynthetic system increased and subsequently decreased with increased nitrogen application rate. The nitrogen ratio between carboxylation and electron transport systems was positively correlated with PNUE in both soybean varieties. The nitrogen ratio in light-harvesting and non-photosynthetic systems showed a linear negative correlation with PNUE.

Conclusions

Overall, an appropriate nitrogen level maintained a high photosynthetic nitrogen ratio, whereas low- or high-nitrogen conditions increased or decreased the nitrogen ratio in non-photosynthetic and photosynthetic systems, respectively, thus decreasing the PNUE and photosynthetic capacity. Moreover, increased nitrogen application rate led to a decreased nitrogen ratio in the light-harvesting system and an increased nitrogen ratio of electron transport and carboxylation systems. Our results provide a theoretical basis for optimizing leaf nitrogen distribution, determining optimum nitrogen levels, and promoting soybean seedling growth.

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施氮水平对大豆幼苗叶片光合氮分布及利用效率的影响

氮营养与作物生长发育密切相关。施氮量影响叶片大小、氮素含量和分布，进而影响光合氮利用效率和产量。以大豆品种“金源55”和“克山1号”为试验材料，以氮肥为尿素，施氮量为:n = 0, 0 kg hm−2;N0.5, 60 kg hm−2;N1, 120 kg hm−2;N1.5, 180 kg hm−2。比较了氮素水平对大豆幼苗形态、生物量、光合生理、氮素分布、PNUE等叶片特性的影响。结果两种大豆品种的最大羧基化和电子转移量、净光合速率和PNUE均随施氮量的增加而显著增加，随后趋于稳定。光合系统中PNUE、羧基化系统组分、电子传递组分和非光合系统分配比例随施氮量的增加先升高后降低。在两个大豆品种中，羧基化系统和电子传递系统之间的氮比与PNUE呈正相关。采光系统和非光合系统的氮素比与PNUE呈线性负相关。结论总体而言，适当的氮素水平维持了较高的光合氮比，而低氮和高氮条件分别增加或降低了非光合系统和光合系统的氮比，从而降低了PNUE和光合能力。氮素施用量的增加导致光收集系统的氮比降低，电子传递系统和羧基化系统的氮比增加。研究结果为优化叶片氮素分布、确定最佳氮素水平、促进大豆幼苗生长提供了理论依据。

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来源期刊

Journal of plant physiology 生物-植物科学

CiteScore

7.20

自引率

4.70%

发文量

196

审稿时长

32 days

期刊介绍： The Journal of Plant Physiology is a broad-spectrum journal that welcomes high-quality submissions in all major areas of plant physiology, including plant biochemistry, functional biotechnology, computational and synthetic plant biology, growth and development, photosynthesis and respiration, transport and translocation, plant-microbe interactions, biotic and abiotic stress. Studies are welcome at all levels of integration ranging from molecules and cells to organisms and their environments and are expected to use state-of-the-art methodologies. Pure gene expression studies are not within the focus of our journal. To be considered for publication, papers must significantly contribute to the mechanistic understanding of physiological processes, and not be merely descriptive, or confirmatory of previous results. We encourage the submission of papers that explore the physiology of non-model as well as accepted model species and those that bridge basic and applied research. For instance, studies on agricultural plants that show new physiological mechanisms to improve agricultural efficiency are welcome. Studies performed under uncontrolled situations (e.g. field conditions) not providing mechanistic insight will not be considered for publication. The Journal of Plant Physiology publishes several types of articles: Original Research Articles, Reviews, Perspectives Articles, and Short Communications. Reviews and Perspectives will be solicited by the Editors; unsolicited reviews are also welcome but only from authors with a strong track record in the field of the review. Original research papers comprise the majority of published contributions.