Journal of plant physiology最新文献

The assimilation of inorganic nitrogen by cluster and proteoid roots of Aspalathus linearis (Burm. f.) R. Dahlgren and Protea cynaroides (L.) L. in nutrient-poor ecosystems 杨梅簇状根和类蛋白根对无机氮的同化。f。)Dahlgren和cynaroides （L.）L.在营养贫乏的生态系统

IF 4.1 3区生物学 Q1 PLANT SCIENCES

Journal of plant physiology

Pub Date : 2026-01-12 DOI: 10.1016/j.jplph.2026.154703

Stian Griebenow , Lida - Mari Groenewald , Nokwanda Makunga , Maik Veste , Paul Hills , Aleysia Kleinert , Alexander Valentine

Certain plant families have evolved cluster (or proteoid) roots, which facilitate their survival in nutrient-poor ecosystems, specifically related to phosphorus impoverished environments, such as in South Africa, South Western Australia and Chile. Most cluster (or proteoid) rooted studies have focused on their capacity for phosphate acquisition, while in nutrient-poor ecosystems along with phosphate, nitrogen is the most limiting for plant growth. The role of cluster (or proteoid) roots in nitrogen nutrition is poorly understood. Therefore, in a field based experiments two cluster/proteoid rooted species, Protea cynaroides (L.) L. and Aspalathus linearis (Burm. f.) R. Dahlgren, the cluster/proteoid root capacity for inorganic nitrogen assimilation and organic nitrogen recycling utilising was assessed utilising an enzymatic approach. It was shown that cluster/proteoid roots are able to assimilate both NH₄⁺ and NO₃⁻ through the enzyme activities of Glutamine synthase (GS) (EC 6.3.1.2) and Nitrate reductase (NR) (EC 1.7.1.1). Additionally, cluster/proteoid roots were also able to recycle amino acids into other useable forms. The assimilation and recycling of inorganic - and organic nitrogen by cluster/proteoid roots along with their capacity for phosphorus mobilisation, provides insight into how cluster/proteoid roots form part of a larger system in which belowground organs are integrated to acquire scarce resources.

某些植物科已经进化出簇状（或类蛋白质）根，这有助于它们在营养贫乏的生态系统中生存，特别是在南非、澳大利亚西南部和智利等磷匮乏的环境中。大多数基于簇（或类蛋白质）的研究都集中在它们获取磷酸盐的能力上，而在营养贫乏的生态系统中，氮和磷酸盐一起是植物生长的最大限制因素。簇状（或类蛋白质）根在氮营养中的作用尚不清楚。因此，在野外实验中，两种簇状/类蛋白质根物种，cynaroides （L.）芦笋和芦笋；f。)R. Dahlgren，利用酶的方法评估了簇/蛋白质根对无机氮同化和有机氮循环利用的能力。结果表明，簇/类蛋白根能够通过谷氨酰胺合成酶（GS）（EC 6.3.1.2）和硝酸还原酶（NR）（EC 1.7.1.1）的酶活性吸收NH4+和NO3−。此外，簇/类蛋白质根也能够将氨基酸循环成其他可用的形式。簇状/类蛋白质根对无机氮和有机氮的同化和再循环，以及它们对磷的动员能力，提供了对簇状/类蛋白质根如何形成一个更大系统的一部分的见解，在这个系统中，地下器官被整合以获取稀缺资源。

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引用次数: 0

Auxin-mediated regulation and functional adaptation of leaf veins under heat stress 生长素对热胁迫下叶脉的调控及功能适应

IF 4.1 3区生物学 Q1 PLANT SCIENCES

Journal of plant physiology

Pub Date : 2026-01-11 DOI: 10.1016/j.jplph.2026.154702

Afroz Naznin , Yuanyuan Wang , Jing He , Md Mazadul Islam , Asad Abbas , Jay Bose , Oula Ghannoum , Zhong-Hua Chen

Elevated global temperatures threaten crop yield and quality by impairing plant hydraulic efficiency and photosynthetic stability, hence highlighting the significance of vascular architectural plasticity in heat stress tolerance. Leaf vein architecture, the principal conduit for water, nutrients, and photosynthates, provides structural support and controls gas exchange, which are critical for sustaining growth and productivity under heat stress. Increasing evidence shows that vascular plasticity, including adjustments in vein density and patterning, underpins plant resilience by maintaining physiological homeostasis. This review summarizes the current knowledge of how heat stress influences leaf and vein structure, with an emphasis on the molecular regulatory networks that drive vascular structural adaptation. We highlight the central role of auxin in coordinating vascular differentiation through its regulation of biosynthesis, polar transport, and signalling transduction, and discuss how auxin integrates with other hormonal pathways to fine-tune vascular traits in response to environmental cues. Particularly, we focus on the unique vein patterning strategies and physiological function in the grass family, including species of many major food and cash crops with agricultural and ecological significance. By integrating these insights, we propose a framework that links vascular plasticity with plant development and yield, offering research insights and practical guidance for breeding heat-resilient crop varieties.

全球气温升高会损害植物的水力效率和光合稳定性，从而威胁作物的产量和质量，因此，维管结构可塑性在耐热性中的重要性凸显出来。叶脉结构是水、养分和光合作用的主要通道，提供结构支持和控制气体交换，这对在热胁迫下维持生长和生产力至关重要。越来越多的证据表明，血管可塑性，包括血管密度和模式的调整，通过维持生理稳态来支撑植物的恢复力。本文综述了目前关于热胁迫如何影响叶片和叶脉结构的知识，重点介绍了驱动血管结构适应的分子调控网络。我们强调了生长素通过调节生物合成、极性运输和信号转导在协调血管分化中的核心作用，并讨论了生长素如何与其他激素途径整合以微调血管性状以响应环境信号。我们特别关注草科植物中独特的静脉模式策略和生理功能，包括许多具有农业和生态意义的主要粮食和经济作物物种。通过整合这些见解，我们提出了一个将维管可塑性与植物发育和产量联系起来的框架，为耐热作物品种的选育提供了研究见解和实践指导。

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引用次数: 0

The cyclin-dependent kinase inhibitor SlKRP3 negatively regulates plant height and fruit shape in tomato via inhibiting cell elongation 周期蛋白依赖性激酶抑制剂SlKRP3通过抑制细胞伸长负向调节番茄植株高度和果实形状

IF 4.1 3区生物学 Q1 PLANT SCIENCES

Journal of plant physiology

Pub Date : 2026-01-10 DOI: 10.1016/j.jplph.2026.154694

Genzhong Liu , Xiaofang Liu , Jiaojiao Fan, Chaoyu Li, Wang Zheng, Fangfang Ma, Zhilong Bao

Plant height and fruit shape are significant traits affecting plant yield and appearance quality. Kip-related protein (KRP) is a cyclin-dependent kinase inhibitor that plays a critical role in the inhibition of cell cycle progression during plant development. However, the mechanism by which SlKRP3 regulates tomato plant height and fruit shape through cell cycle progression remains unclear. Here, we unveil functional characterization of SlKRP3, which is responsible for plant height and fruit shape in tomato. As expected, overexpression of SlKRP3 resulted in shorter cell elongation and decreased endoreduplication in the tomato stem. VIGS assay was performed to obtain SlKRP3-silenced plants and demonstrated that silencing of SlKRP3 increased plant height. Transcriptome analysis showed that the xyloglucosyl transferase genes are also dysregulated in SlKRP3 overexpression lines, as are cell elongation and cell cycle-related genes. This argues that SlKRP3 negatively regulates cell expansion via inhibiting endoreduplication in tomato. Notably, we uncover that SlKRP3 physically interacted with cyclin D3.1 by AlphaFold3, yeast two-hybrid, and bimolecular fluorescence complementation (BiFC) assays. These findings shed light on the functional regulation of SlKRP3 and offer potential strategies for the genetic improvement of plant architecture and fruit shape in tomato.

株高和果形是影响植株产量和外观品质的重要性状。kip相关蛋白（KRP）是一种周期蛋白依赖性激酶抑制剂，在植物发育过程中对细胞周期进程的抑制起关键作用。然而，SlKRP3通过细胞周期进程调控番茄植株高度和果实形状的机制尚不清楚。在这里，我们揭示了SlKRP3的功能特征，它负责番茄的株高和果实形状。正如预期的那样，SlKRP3的过表达导致番茄茎中的细胞伸长缩短和内复制减少。通过VIGS实验获得了SlKRP3沉默植株，结果表明SlKRP3沉默增加了植株的高度。转录组分析显示，在SlKRP3过表达系中，木糖基转移酶基因也出现了异常，细胞伸长和细胞周期相关基因也出现了异常。这表明SlKRP3通过抑制番茄内复制负向调节细胞扩增。值得注意的是，我们通过AlphaFold3、酵母双杂交和双分子荧光互补（BiFC）实验发现，SlKRP3与细胞周期蛋白D3.1存在物理相互作用。这些发现揭示了SlKRP3的功能调控，为番茄植株结构和果实形状的遗传改良提供了潜在的策略。

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引用次数: 0

Screening frost-tolerant kale (Brassica oleracea L. var. acephala) genotypes through cold-responsive metabolic changes in open field conditions 通过大田条件下冷响应代谢变化筛选耐冻甘蓝基因型

IF 4.1 3区生物学 Q1 PLANT SCIENCES

Journal of plant physiology

Pub Date : 2026-01-09 DOI: 10.1016/j.jplph.2026.154695

Lovro Sinkovič , Barbara Pipan , Mohamed Neji , Hajer Ben Ammar , Vladimir Meglič , Robert Veberič , Ana Slatnar , Jerneja Jakopič

Kale (Brassica oleracea L. var. acephala) is a cold-tolerant leafy vegetable whose metabolic plasticity under frost stress remains underexplored. In this study, leaf tissues from 26 kale accessions grown under open-field conditions were metabolically profiled, focusing on soluble sugars, glucosinolates, and photosynthetic pigments before and after exposure to short-term frost. Frost stress induced significant quantitative and compositional shifts in sugar profiles, notably an accumulation of sucrose, suggesting its role as a key osmoprotectant. Among the twelve glucosinolates identified, indolic compounds dominated the unfrosted profile (67.4 %) but declined post-frost (51.6 %), coinciding with a marked increase in aliphatic glucosinolates (from 20.7 % to 38.6 %). Chlorophyll and carotenoid contents declined in most accessions following frost exposure. Notably, the metabolic profile of Accession_4 indicates a potentially resilient phenotype, characterised by limited pigment degradation and a shift toward aliphatic glucosinolates. This may reflect a stress-adaptation strategy and could be explored as a candidate for breeding or metabolotype selection approaches.

羽衣甘蓝（Brassica oleracea L. var. acephala）是一种耐寒的叶菜，其在霜冻胁迫下的代谢可塑性尚不充分。在本研究中，对26份在露天条件下生长的羽衣甘蓝叶片组织进行了代谢分析，重点研究了短期霜冻前后可溶性糖、硫代葡萄糖苷和光合色素的代谢变化。霜冻胁迫诱导糖谱发生了显著的数量和成分变化，尤其是蔗糖的积累，这表明它是一种关键的渗透保护剂。在鉴定的12种硫代葡萄糖苷中，吲哚类化合物在未霜冻的情况下占主导地位（67.4%），但霜冻后含量下降（51.6%），与脂肪族硫代葡萄糖苷显著增加（从20.7%增加到38.6%）相一致。多数品种的叶绿素和类胡萝卜素含量在霜冻后下降。值得注意的是，Accession_4的代谢谱显示出一种潜在的弹性表型，其特征是有限的色素降解和向脂肪族硫代葡萄糖苷的转变。这可能反映了一种压力适应策略，可以作为育种或代谢型选择方法的候选物。

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引用次数: 0

ABA receptors: function and post-translational modifications in plants ABA受体：在植物中的功能和翻译后修饰

IF 4.1 3区生物学 Q1 PLANT SCIENCES

Journal of plant physiology

Pub Date : 2026-01-08 DOI: 10.1016/j.jplph.2026.154692

Shaotong Qin, Qianqian Qin, Suiwen Hou

The plant hormone abscisic acid (ABA) plays an important role in plant growth, development and abiotic stresses. ABA perception is mediated by its receptors, PYRABACTIN RESISTANCE 1 (PYR1)/PYR1-like (PYL) proteins (collectively referred to as PYLs), which initiate downstream ABA signaling. The functional regulation of PYLs, particularly through post-translational modifications (PTMs) is gradually attracting extensive attention. Here, we have summarized recent advances in research on PTMs of PYL family, highlighting how mechanisms such as phosphorylation, ubiquitination and nitration fine-tune their activity, stability and subcellular localization. We also briefly review the biological function and genetic phenotypes of PYL family, underscoring their central role in ABA signaling and stress adaptive responses in plants. Future studies should address key questions regarding the additional PTMs, specific sites and crosstalk of these PTMs.

植物激素脱落酸（ABA）在植物生长发育和非生物胁迫中起着重要作用。ABA感知是由其受体PYRABACTIN RESISTANCE 1 (PYR1)/PYR1样（PYL）蛋白（统称PYL）介导的，这些蛋白启动下游ABA信号传导。pyl的功能调控，尤其是通过翻译后修饰（post-translational modification, PTMs）的功能调控逐渐受到广泛关注。本文综述了PYL家族PTMs的最新研究进展，重点介绍了磷酸化、泛素化和硝化等机制对其活性、稳定性和亚细胞定位的调控作用。我们还简要回顾了PYL家族的生物学功能和遗传表型，强调了它们在植物ABA信号传导和逆境适应反应中的核心作用。未来的研究应解决的关键问题，关于额外的PTMs，具体的位置和这些PTMs的串扰。

引用次数: 0

Functional analysis of SlCDPK12 and its interacting protein SlACS11 in the regulation of tomato resistance to Phytophthora infestans SlCDPK12及其互作蛋白SlACS11调控番茄对疫霉抗性的功能分析

IF 4.1 3区生物学 Q1 PLANT SCIENCES

Journal of plant physiology

Pub Date : 2026-01-05 DOI: 10.1016/j.jplph.2026.154689

Yan Li , Jiaxuan Zhu , Ruili Lv, Zhengjie Wang, Huimin Li, Ruirui Yang, Yushi Luan

Tomato (Solanum lycopersicum) is a globally important economic vegetable crop, but its growth and yield are often limited by biotic stresses. Calcium-dependent protein kinases (CDPKs), as key components of the Ca²⁺ signaling pathway, play crucial roles in plant stress responses and have been extensively studied in relation to biotic stress adaptation. However, the specific role of CDPKs in tomato resistance to late blight (Phytophthora infestans) remains largely unclear. In this study, we demonstrated that SlCDPK12 functions as a positive regulator of tomato resistance to P. infestans. Overexpression of SlCDPK12 in the P. infestans-susceptible cultivar ZaoFen No.2 significantly enhanced resistance. This enhanced resistance was accompanied by elevated expression of pathogenesis-related (PR) genes and increased accumulation of reactive oxygen species (ROS). Using yeast two-hybrid screening, we identified SlACS11, a member of the 1-aminocyclopropane-1-carboxylic acid synthase (ACS) family, as a candidate interacting protein of SlCDPK12. Intriguingly, transient silencing of SlACS11 enhanced tomato resistance to P. infestans. Overall, this research provides new insights into the molecular mechanisms underlying tomato resistance to P. infestans and contributes to our understanding of the biological functions of the CDPK gene family in plant-pathogen interactions.

番茄（Solanum lycopersicum）是全球重要的经济蔬菜作物，但其生长和产量往往受到生物胁迫的限制。钙依赖性蛋白激酶（Calcium-dependent protein kinase, CDPKs）作为Ca2+信号通路的关键组分，在植物的逆境响应中起着至关重要的作用，并与生物逆境适应有关。然而，CDPKs在番茄抗晚疫病（疫霉）中的具体作用仍不清楚。在这项研究中，我们证明了SlCDPK12作为番茄对病原菌抗性的正调节因子。SlCDPK12的过表达显著增强了耐药品种早粉2号的抗性。这种抗性的增强伴随着致病相关基因（PR）的表达升高和活性氧（ROS）的积累增加。通过酵母双杂交筛选，我们确定了1-氨基环丙烷-1-羧酸合成酶（ACS）家族成员SlACS11作为SlCDPK12的候选相互作用蛋白。有趣的是，SlACS11的短暂沉默增强了番茄对病原菌的抗性。总的来说，这项研究为番茄抵抗病原菌的分子机制提供了新的见解，并有助于我们了解CDPK基因家族在植物-病原体相互作用中的生物学功能。

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引用次数: 0

Molecular mechanisms and biotechnological advances in herbicide resistance: Insights into the development of herbicide-tolerant crops 抗除草剂的分子机制和生物技术进展：对耐除草剂作物发展的见解。

IF 4.1 3区生物学 Q1 PLANT SCIENCES

Journal of plant physiology

Pub Date : 2026-01-05 DOI: 10.1016/j.jplph.2026.154690

Sheng Zheng , Haiyan Tie , Shangli Chai, Mingxiao Wang, Sirui Wang, Yuan-Yuan Zeng, Guofan Wu, Teng-Guo Zhang

Herbicides play a pivotal role in modern agriculture by controlling weed populations and safeguarding crop yields. However, the long-term and extensive use of herbicides has accelerated the evolution of herbicide-resistant weeds, thereby diminishing their efficacy and posing a serious threat to global food security. Recent advances in molecular biology and plant biotechnology have greatly expanded our understanding of herbicide resistance mechanisms and enabled the development of crops with enhanced herbicide resistance. Herbicide resistance genes function primarily by encoding detoxifying enzymes, modifying herbicide target sites, or activating specific metabolic pathways that mitigate herbicidal toxicity. Emerging genetic tools, including transgenic approaches and CRISPR/Cas-mediated genome editing, have further facilitated the precise introduction of resistance traits into major crops. It is noteworthy that this review offers novel insights into the latest CRISPR/Cas applications, including base editing and prime editing for developing novel, non-transgenic herbicide-resistant crops. Furthermore, it provides a systematic overview of advanced strategies for engineering multi-gene stacking traits to combat complex or evolving weed resistance. This review integrates recent progress in elucidating the molecular targets of herbicides and the underlying resistance mechanisms, and highlights the potential of modern biotechnological strategies for engineering herbicide-resistant crops to promote sustainable and environmentally responsible weed management.

除草剂在现代农业中起着控制杂草数量和保障作物产量的关键作用。然而，长期和广泛使用除草剂加速了抗除草剂杂草的进化，从而降低了其药效，对全球粮食安全构成严重威胁。分子生物学和植物生物技术的最新进展极大地扩展了我们对除草剂抗性机制的理解，并使开发具有增强抗除草剂能力的作物成为可能。除草剂抗性基因主要通过编码解毒酶、修饰除草剂靶点或激活特定的代谢途径来减轻除草剂毒性。新兴的遗传工具，包括转基因方法和CRISPR/ cas介导的基因组编辑，进一步促进了将抗性性状精确引入主要作物。值得注意的是，本综述为CRISPR/Cas的最新应用提供了新的见解，包括碱基编辑和引体编辑在开发新型非转基因抗除草剂作物中的应用。此外，它提供了一个系统的概述了先进的策略，工程多基因堆叠性状，以对抗复杂的或不断发展的杂草抗性。本文综述了除草剂分子靶点和潜在抗性机制的最新研究进展，并强调了现代生物技术策略在抗除草剂作物工程方面的潜力，以促进可持续和对环境负责的杂草管理。

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引用次数: 0

Coordinated systemic regulation maintains plant nutrient homeostasis 协调的系统调节维持植物营养平衡

IF 4.1 3区生物学 Q1 PLANT SCIENCES

Journal of plant physiology

Pub Date : 2026-01-05 DOI: 10.1016/j.jplph.2026.154691

Qian-Qian Li, Yi-Qun Gao

Plant survival in fluctuating environments depends on the precise maintenance of nutrient homeostasis, which requires the integration of local nutrient availability with whole-plant demand. While the molecular components of local nutrient sensing and uptake are well-characterized, the systemic signaling networks that coordinate acquisition, allocation, and utilization across distant organs are less understood. This review synthesizes recent advances in understanding the systemic regulation of plant nutrient homeostasis. We highlight how plants coordinate photosynthetic activity in shoots with mineral nutrient absorption in roots to optimize growth. Furthermore, we examine the long-distance communication systems through which diverse mobile signals are translocated via the vasculature to synchronize root nutrient uptake with shoot photosynthetic status and developmental demands. Collectively, this synthesis illuminates the complex and finely tuned regulatory mechanisms that balance growth with environmental adaptation.

植物在波动环境中的生存依赖于营养稳态的精确维持，这需要将当地的营养供应与整个植物的需求结合起来。虽然局部营养感知和吸收的分子成分已被很好地表征，但协调远端器官获取、分配和利用的系统信号网络尚不清楚。本文综述了植物营养平衡系统调控的最新研究进展。我们强调了植物如何协调芽部的光合作用与根系的矿质养分吸收以优化生长。此外，我们还研究了远距离通信系统，通过该系统，各种移动信号通过脉管系统进行转运，以同步根营养吸收与茎部光合状态和发育需求。总的来说，这种综合阐明了平衡生长与环境适应的复杂而精细的调节机制。

引用次数: 0

Multi-omics profiling identifies potential biological nitrification inhibitor 1,9-decanediol biosynthesis and secretion mechanisms in diverse rice varieties 多组学分析鉴定了不同水稻品种潜在的生物硝化抑制剂1,9-十二烷二醇的生物合成和分泌机制。

IF 4.1 3区生物学 Q1 PLANT SCIENCES

Journal of plant physiology

Pub Date : 2026-01-02 DOI: 10.1016/j.jplph.2026.154688

Dong-Wei Di , Chi-Shang Luan , Ming-Kun Ma , Ting Yang , Herbert J. Kronzucker , Ju Min , Yufang Lu , Xiaofei Liu , Weiming Shi

1,9-Decanediol (1,9-D) was identified as the first fatty alcohol with biological nitrification inhibition (BNI) activity in rice root exudates. In a previous study, transcriptomic and untargeted metabolomic analyses contrasted two rice varieties, suggesting metabolic pathways and associated gene families involved in 1,9-D biosynthesis and secretion. However, due to the small number of varieties and technical constraints, the pool of candidate genes remained extensive, impeding precise identification and functional validation. Here, we employ ten rice varieties with diverging root-secretion properties for integrated transcriptomic and targeted metabolomic profiling. Our results demonstrate that ammonium treatment significantly enhances 1,9-D secretion across varieties, indicating a potentially conserved regulatory mechanism in rice. Integrated analysis revealed that α-linolenic acid (LN) and linoleic acid (LA) may serve as biosynthetic precursors of 1,9-D and identified key candidate genes in LN/LA metabolism, including Os04g37430, Os04g47120, Os08g39840, Os08g39850, and Os08g08220. Furthermore, our data show that root secretion of 1,9-D is actively regulated by specific transporters, including the major facilitator superfamily (MFS) member Os11g04104, the ATP-binding cassette (ABC) transporter Os01g07870, and the multidrug and toxic compound extrusion (MATE) transporter Os10g20390. This study reveals a novel multi-gene regulatory network underlying 1,9-D synthesis and secretion, providing candidate targets for improving nitrogen-use efficiency in rice.

1,9-癸二醇（1,9- d）是水稻根系分泌物中第一个具有生物硝化抑制（BNI）活性的脂肪醇。在之前的研究中，转录组学和非靶向代谢组学分析对比了两个水稻品种，发现了参与1,9- d生物合成和分泌的代谢途径和相关基因家族。然而，由于品种较少和技术限制，候选基因池仍然广泛，阻碍了精确鉴定和功能验证。在这里，我们采用了10个具有不同根分泌特性的水稻品种进行综合转录组学和靶向代谢组学分析。我们的研究结果表明，铵处理显著提高了水稻品种间1,9- d的分泌，表明了一个潜在的保守调控机制。综合分析发现α-亚麻酸（LN）和亚油酸（LA）可能是1,9- d的生物合成前体，并鉴定出参与LN/LA代谢的关键候选基因Os04g37430、Os04g47120、Os08g39840、Os08g39850和Os08g08220。此外，我们的数据显示，1,9- d的根分泌受到特定转运体的积极调节，包括主要促进剂超家族（MFS）成员Os11g04104、atp结合盒（ABC）转运体Os01g07870和多药和有毒化合物挤出（MATE）转运体Os10g20390。该研究揭示了1,9- d合成和分泌的一个新的多基因调控网络，为提高水稻氮素利用效率提供了候选靶点。

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引用次数: 0

Localization of heterosis loci for quality traits and identification of candidate genes in Brassica napus 甘蓝型油菜品质性状杂种优势位点定位及候选基因鉴定。

IF 4.1 3区生物学 Q1 PLANT SCIENCES

Journal of plant physiology

Pub Date : 2025-12-31 DOI: 10.1016/j.jplph.2025.154687

Guoqiang Zheng , Ying Wang , Jiaping Wei , Zefeng Wu , Jinxiong Wang , Qi Yang , Junmei Cui , Yan Fang , Xiaoyun Dong , Xinyi Zhang , Qian Luo , Jiayue Yang , Zigang Liu

The quality traits of rapeseed are critical genetic characteristics that determine seed value and its applications. Heterosis manifests not only in yield traits but also in quality traits. In this study, multiple interspecific and intraspecific hybrid crosses were generated to evaluate their heterosis effects. Our results demonstrated that the heterosis of oil content, oleic acid, linoleic acid, and linolenic acid was higher in the winter × spring crosses than in the winter × winter crosses, whereas the opposite was true for erucic acid and glucosinolate. Furthermore, we identified a substantial numerous SNP markers through targeted sequencing and mapped the heterosis-related loci for the corresponding traits in the F₁ population. A lot of 233, 46, 247, 192, 203, and 64 QTLs were identified as being associated with the seed traits of erucic acid, glucosinolates, oleic acid, linoleic acid, linolenic acid, oil content and their heterosis. These were consolidated into 425 consensus QTLs (cq-QTLs). By integrating RNA-seq data, we identified 10 pathways involved in lipid and pyruvate metabolism, among which 28 candidate genes were annotated. These conclusions showed that these cq-QTLs can serve as hotspots for mining regulatory genes related to the heterosis of rapeseed quality traits, laying a foundation for research on the heterosis of rapeseed quality traits.

油菜籽品质性状是决定油菜籽价值及其应用的关键遗传性状。杂种优势不仅表现在产量性状上，也表现在品质性状上。本研究通过建立多个种间和种内杂交组合来评价其杂种优势效应。结果表明，冬×春杂交中油酸、亚油酸和亚麻酸的杂种优势高于冬×冬杂交，而芥酸和硫代葡萄糖苷的杂种优势则相反。此外，我们通过靶向测序鉴定了大量SNP标记，并绘制了F1群体中相应性状的杂种优势相关位点。其中233个、46个、247个、192个、203个和64个qtl与芥酸、硫代葡萄糖苷、油酸、亚油酸、亚麻酸、含油量及其杂种优势性状相关。这些被整合成425个共识qtl （cq- qtl）。通过整合RNA-seq数据，我们确定了10个参与脂质和丙酮酸代谢的途径，其中28个候选基因被注释。综上所述，这些cq- qtl可作为油菜籽品质性状杂种优势相关调控基因挖掘的热点，为油菜籽品质性状杂种优势研究奠定基础。

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引用次数: 0