Journal of plant physiology最新文献

{"title":"AmCERK1 and AmLYK3 interaction mediates CIP-induced defense responses in A. macrocephala","authors":"Lei Chen ,&nbsp;Xuyan Fu ,&nbsp;Kun Wu ,&nbsp;Xiangbing Chang ,&nbsp;Wei Tian","doi":"10.1016/j.jplph.2025.154497","DOIUrl":"10.1016/j.jplph.2025.154497","url":null,"abstract":"<div><div>Southern blight caused by <em>Sclerotium rolfsii</em> (<em>S. rolfsii</em>) represents a significant threat to the medicinal plant <em>Atractylodes macrocephala</em> Koidz. (<em>A. macrocephala</em>), with effective control measures remaining limited. <em>Chrysanthemum indicum</em> polysaccharides (CIP) have been identified as an elicitor capable of inducing defense responses in <em>A. macrocephala</em> against <em>S. rolfsii</em> infection. However, the molecular mechanisms underlying CIP recognition remain poorly understood. In this study, comparative transcriptome analysis revealed two potential <em>LysM</em>-receptor kinases, Am<em>CERK1</em> and Am<em>LYK3</em>, as candidate receptors for CIP recognition. These genes, which are orthologous to <em>Arabidopsis CERK1</em> and <em>Medicago truncatula LYK3</em>, exhibited significant up-regulation upon CIP treatment. Bimolecular fluorescence complementation (BiFC) assays demonstrated that Am<em>CERK1</em> and Am<em>LYK3</em> interact in a CIP-dependent manner. Transient overexpression experiments further confirmed that CIP treatment markedly enhanced the expression of these receptor genes. Virus-induced gene silencing (VIGS) assays indicated that CIP treatment could partially compensate for the suppression of Am<em>CERK1</em> and Am<em>LYK3</em>, highlighting their critical role in CIP-induced defense responses. Collectively, these findings suggest that Am<em>CERK1</em> and Am<em>LYK3</em> form a pattern recognition receptor (PRR) complex essential for CIP perception, potentially facilitating pattern-triggered immunity (PTI) in <em>A. macrocephala</em>. These findings reveal a novel receptor recognition complex comprising Am<em>CERK1</em> and Am<em>LYK3</em>, offering crucial insights into the mechanisms of innate immune recognition in plants.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"308 ","pages":"Article 154497"},"PeriodicalIF":4.0,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143863560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Xanthophyll cycle and photosynthetic electron transport enhanced by galactolipid modification alleviate drought-induced leaf senescence","authors":"Jili Xu ,&nbsp;Guanqiang Zuo ,&nbsp;Shuaikang Liu ,&nbsp;Hao Shi ,&nbsp;Lina Yin ,&nbsp;Shiwen Wang ,&nbsp;Xiping Deng","doi":"10.1016/j.jplph.2025.154499","DOIUrl":"10.1016/j.jplph.2025.154499","url":null,"abstract":"<div><div>Previous studies have demonstrated galactolipid modification was involved in drought-induced leaf senescence. Under drought stress, overactivation of the photosynthetic electron transfer chain leads to excessive light energy absorption, resulting in photooxidative damage to crops. The xanthophyll cycle, a key photoprotective mechanism, mitigates light-induced damage by dissipating excess energy as heat. However, the role of the xanthophyll cycle pigments and photosynthetic electron transport in the process of galactolipid modification alleviates drought-induced leaf senescence has not yet been clarified clearly. In this study, a comparative experiment was conducted to investigate changes in the xanthophyll cycle and photosynthetic electron transport during drought and re-watering in two maize varieties: a drought-tolerant variety (Liangyu66) and a senescent variety (Liangyu99). Drought stress induced more severely wilted and leaf senescence in Liangyu99, with lower shoot biomass, photosynthetic rate, chlorophyll <em>a</em>/b, monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG) content, corresponding gene expression level and DGDG/MGDG ratio compared to Liangyu66. Furthermore, PSII electron transport rate (ETRⅡ), the PSI electron transport rate (ETRⅠ), and cyclic electron flow (CEF) in Liangyu66 were 14 %, 47 %, and 83 % higher, respectively, than in Liangyu99 under drought stress. Notably, the de-epoxidation state of the xanthophyll cycle (A + Z)/(A + Z + V) was significantly higher in Liangyu66 than in Liangyu99. Non-photochemical quenching (NPQ) increased in both varieties under drought stress, Liangyu66 displayed a higher NPQ than Liangyu99. These findings suggest that galactolipid modification alleviates drought-induced leaf senescence by enhancing the xanthophyll cycle and optimizing photosynthetic electron transport.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"308 ","pages":"Article 154499"},"PeriodicalIF":4.0,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143859224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overexpression of FvGCN5 enhances the resistance of woodland strawberry against Botrytis cinerea","authors":"Miao Yu,&nbsp;Jiaqi Zhang,&nbsp;Feifei Bai,&nbsp;Yihan Gao,&nbsp;Shan Jiang,&nbsp;Hui Liu,&nbsp;Aisheng Xiong,&nbsp;Zongming Cheng,&nbsp;Jinsong Xiong","doi":"10.1016/j.jplph.2025.154496","DOIUrl":"10.1016/j.jplph.2025.154496","url":null,"abstract":"<div><div>Epigenetic modifications mediated by histone acetylation play essential roles in plant development and stress response. However, the mechanism of regulating biotic stress through histone acetyltransferase GCN5 in strawberry is still unclear. In this study, we isolated <em>FvGCN5</em> from woodland strawberry and found that FvGCN5 may form the conserved SAGA (Spt-Ada-Gcn5 acetyltransferase) complex through interaction with FvADA2a and FvADA2b. In addition, we found that <em>FvGCN5</em> could be significantly induced by the infection of fungal pathogen <em>Botrytis cinerea</em>, and that the transgenic strawberry plants overexpressing <em>FvGCN5</em> exhibited enhanced resistance against <em>B. cinerea</em>. Further RNA-seq-based transcriptome and quantitative PCR analysis indicated that several disease-resistant genes such as <em>FvMYC2</em> and, <em>FvPR1</em> were significantly upregulated in <em>FvGCN5</em> overexpression lines. Taken together, our study indicates that <em>FvGCN5</em> plays important roles in the resistance against <em>B. cinerea</em> in woodland strawberry through activating disease-resistant genes.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"308 ","pages":"Article 154496"},"PeriodicalIF":4.0,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143859223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative transcriptome and metabolome analyses reveal the molecular mechanism of re-flowering induction in Hydrangea macrophylla","authors":"Haixia Chen ,&nbsp;Huijun Zhang ,&nbsp;Denghui Wang ,&nbsp;Yajing Wang,&nbsp;Hui Jiang,&nbsp;Jiren Chen","doi":"10.1016/j.jplph.2025.154492","DOIUrl":"10.1016/j.jplph.2025.154492","url":null,"abstract":"<div><div>Flowering duration is pivotal for ornamental appeal, with re-flowering being essential for prolonging the decorative period and enhancing the aesthetics of flowers. We conducted transcriptome and metabolome sequencing analyses on the primary and secondary flower buds of <em>H. macrophylla</em> cv 'White Angel', aiming to reveal the molecular regulatory mechanism of secondary flowering. Results showed that the key MADS-box transcription factor family genes closely related to flowering regulation such as <em>AGL42</em>, <em>AGL24</em>, and <em>SVP</em> demonstrated a substantial increase in expression levels within the secondary flower buds. The up-regulation of these genes may promote the transition from vegetative growth to reproductive growth by regulating the expression of downstream target genes, thus triggering secondary flowering. In addition, genes related to starch and sucrose metabolism (such as TPS and TPP) were significantly overexpressed in secondary flower buds, promoting the accumulation of energy metabolites such as Trehalose-6p, Trehalose and D-Glucose, which may create conditions for secondary flowering by providing necessary energy support. At the same time, terpenoid biosynthesis-related genes (such as <em>KO</em>, <em>KAO</em>, <em>GA2ox</em> and <em>GA3ox)</em> were highly expressed in secondary flower buds, significantly increasing the contents of GA4 and GA7, while decreasing the level of GA3. These dynamic changes of gibberellins (GAs) may regulate the expression of flowering related genes. Further promote the occurrence of secondary flowering. In summary, this study revealed the synergistic effect of genes and metabolites in the regulation of secondary flowering of <em>Hydrangea macrophylla</em> 'White Angel', and the MADS-box transcription factor directly promoted the transformation of reproductive growth through up-regulated expression. The accumulation of starch, sucrose and its derivatives and gibberellin metabolites may trigger the secondary flowering process of plants through energy supply and hormone signal regulation. These findings provide a new perspective for in-depth analysis of the flowering regulation mechanism of <em>Hydrangea macrophylla</em>, and lay a theoretical foundation for further cultivation of horticultural varieties with excellent ornamental characteristics.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"308 ","pages":"Article 154492"},"PeriodicalIF":4.0,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143844509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulatory network of lncRNAs and mRNAs explains why salinity promotes photosynthesis and plant growth in the halophyte Suaeda salsa","authors":"Chenyang Li ,&nbsp;Runtai Zhao ,&nbsp;Bing Cui ,&nbsp;Ranran Liu ,&nbsp;Chaoran Shi ,&nbsp;Jie Song","doi":"10.1016/j.jplph.2025.154493","DOIUrl":"10.1016/j.jplph.2025.154493","url":null,"abstract":"<div><div><em>Suaeda salsa</em> L. exhibits strong salt tolerance, with 200 mM NaCl being the optimum salt concentration for its growth. However, the specific molecular regulatory network remains unclear. This study used high-throughput sequencing technology to identify the expression abundance of lncRNAs after 24 h of 200 mM NaCl treatment (S24). A total of 16533 novel_lncRNAs were obtained, mainly divided into 10764 lincRNAs (65.11 %), 4936 antisense (29.85 %), and 833 sense overlap (5.04 %). Comparing lncRNAs at S24 and CK revealed 231 up-regulated and 257 down-regulated lncRNAs detected. The differential target genes corresponding to lncRNAs were mainly enriched in carbon metabolism, glycolysis/gluconeogenesis, carbon fixation in photosynthetic organisms and glyoxylate and dicarboxylate metabolism. Comparing the mRNAs at S24 and CK, the up-and down-regulated genes were 998 and 776, respectively, which corresponded to those for lncRNAs. Further investigation revealed that a particular lncRNA, TCONS_00024624 (lnc24), interacts with three genes that collectively regulate ribulose bisphosphate carboxylase (Rubisco). The expression of target genes of lncRNAs and activity of Rubisco and GAPDH in the leaves of <em>S. salsa</em> were upregulated and increased at S24 compared with CK. In conclusion, the results suggest that lncRNAs play important roles in enhancing the photosynthetic capacity of <em>S. salsa</em> and promoting its growth at 200 mM NaCl. This provides new references for studying salt tolerance mechanisms in <em>S. salsa</em>.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"308 ","pages":"Article 154493"},"PeriodicalIF":4.0,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143844507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Is the redox state of the PQ pool involved in regulating the ET biosynthesis pathway of CAM facultative semi-halophytes?","authors":"Miron Gieniec ,&nbsp;Zbigniew Miszalski ,&nbsp;Piotr Rozpądek ,&nbsp;Roman J. Jędrzejczyk ,&nbsp;Isabel Nogues ,&nbsp;Emanuele Pallozzi ,&nbsp;Walter Stefanoni ,&nbsp;Michał Nosek","doi":"10.1016/j.jplph.2025.154491","DOIUrl":"10.1016/j.jplph.2025.154491","url":null,"abstract":"<div><div>The mechanisms and factors regulating ethylene (ET) biosynthesis and its action remain largely unexplored, particularly in CAM-facultative and (semi)halophytic plants. The use of <em>Mesembryanthemum crystallinum</em> L. (ice plant) provides a unique opportunity to examine plastoquinone (PQ) - ET interactions in semi-halophytes and CAM-facultative plants simultaneously. Here, we present the results of an analysis of the common ice plant's response to prolonged (14-day) salinity stress and DCMU, which maintains the PQ pool in a more oxidised state, thereby mimicking darkness conditions. Differentially expressed gene (DEG) analysis showed that the expression of genes involved in ET regulation was not significantly altered after DCMU application. However, in C₃ plants not affected by salinity stress, the expression of genes related to both photosystems, photosynthesis, and the photosynthetic electron transport chain was significantly affected by DCMU. We propose that sustained salinity stress and the occurrence of CAM photosynthesis render physiological processes insensitive to disruptions caused by a modified PQ pool redox state. The UPLC-MS analysis of the ET biosynthesis pathway central intermediate – 1-aminocyclopropane-1-carboxylic acid (ACC) – confirmed the molecular analysis results, as ACC content was similarly affected in salinity untreated and treated plants. Moreover, the analysis of key antioxidative system components, namely catalase and superoxide dismutases, suggests that PQ pool redox state does not directly regulate them. Instead, an alternative regulation mechanism involving reactive oxygen species (ROS) accumulation and a ROS-induced signalling cascade has been proposed.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"308 ","pages":"Article 154491"},"PeriodicalIF":4.0,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143826481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ionome profiling discriminate genotype-dependent responses to drought in durum wheat","authors":"Giulia Quagliata ,&nbsp;Andrea Ferrucci ,&nbsp;Miriam Marín-Sanz ,&nbsp;Francisco Barro ,&nbsp;Gianpiero Vigani ,&nbsp;Stefania Astolfi","doi":"10.1016/j.jplph.2025.154487","DOIUrl":"10.1016/j.jplph.2025.154487","url":null,"abstract":"<div><div>Low-resource environments, such as dry or infertile soils, result in limited plant growth and development, which in turn constrain crop productivity. Water shortage is a significant threat to agricultural productivity all over the world. Drought may also affect plant nutrient uptake and assimilation capability causing nutrient deficiencies even in fertilized fields. Durum wheat is an important staple food crop for ensuring food security in the Mediterranean area, which is increasingly subjected to periods of severe drought due to global changes. Thus, identifying wheat cultivars/genotypes able to cope with suboptimal water, and with unbalanced nutrient availability deriving from drought is crucial to mitigate climate change's adverse effects on agriculture.</div><div>In this study, a detailed analysis of the phenome, including biomass production, proline production, and characterization of root system architecture, and the ionome, was performed on a panel of 15 Triticum turgidum genotypes, differing for drought tolerance, in order to understand the genotype-specific physiological responses to drought and to identify those genotypes characterised by a positive correlation between ion homeostasis and drought response. The characterization of root system architecture helped our understanding of the morphological responses of wheat plants to drought. Our findings demonstrated that drought exposure for 7 days significantly impacted the ionomic profiles of most genotypes in both shoot and root tissues, albeit to varying degrees. The Lcye A⁻B^- genotype showed the highest accumulation efficiency for most nutrients in shoots, while Bulel tritordeum and Karim in roots. It is also important to understand how micronutrients interact with each other and with macronutrients. Thus, we performed a nutrient correlation network analysis, which showed that drought altered the interactions between nutrients in most genotypes. These findings underscore the importance of understanding the mechanisms regulating nutrient homeostasis, as these mechanisms can either mitigate or exacerbate the impact of drought stress.</div><div>Understanding the interplay between ionomic profiles and environmental conditions can provide valuable insights into developing more resilient crops that can thrive in challenging environments, ultimately contributing to global food security in the face of climate change.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"308 ","pages":"Article 154487"},"PeriodicalIF":4.0,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143815881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functions of plant hormones and calcium signaling in regulating root hydrotropism","authors":"Huimin Liang ,&nbsp;Ling Wang ,&nbsp;Fuqiang Gong ,&nbsp;Jinke Chang","doi":"10.1016/j.jplph.2025.154490","DOIUrl":"10.1016/j.jplph.2025.154490","url":null,"abstract":"<div><div>Hydrotropism enables plant roots to grow toward areas with high water availability. This capacity is essential for plant growth and development, particularly when water availability is a limiting factor. The physiological characterization of hydrotropism began approximately 270 years ago, and substantial progress has been made in elucidating its molecular mechanisms over the past two decades. Auxin, cytokinin, abscisic acid, brassinosteroid, and calcium have been reported by various laboratories to regulate root hydrotropism. However, the interrelation among these regulatory components in controlling root hydrotropism remains unknown. This review summarized the regulatory mechanisms of hydrotropism from the perspective of plant hormones and calcium, aiming to elucidate the internal cross-talks between their signaling pathways. Additionally, we addressed central scientific questions, provided insights into future research directions, and highlighted strategies for advancing the application of root hydrotropism in agricultural breeding.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"308 ","pages":"Article 154490"},"PeriodicalIF":4.0,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143760102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soluble sugars maintain redox homeostasis and accelerate the growth of cultured Malva neglecta cells under 2D-clinorotation","authors":"Somayeh Alikhani ,&nbsp;Faezeh Ghanati ,&nbsp;Zahra Hajebrahimi ,&nbsp;Maryam Soleimani ,&nbsp;Naba Najar ,&nbsp;Elham Khalili","doi":"10.1016/j.jplph.2025.154489","DOIUrl":"10.1016/j.jplph.2025.154489","url":null,"abstract":"<div><div>In addition to their nutritional role, carbohydrates play essential roles in metabolism, growth, development, and response to the environment. In the present study, the effects of clinorotation on structural and soluble sugar metabolism and the redox system were investigated in cultured <em>Malva neglecta</em> cells. A rapidly growing cell line was established from leaf explants of <em>M. neglecta</em> on a solidified LS medium, and the cells were exposed to 2D-clinostat for 7 days. Clinorotation significantly increased monosaccharide content, including glucose, fructose, rhamnose, mannose, and xylose, while reducing sucrose levels compared to control groups. The activities of pectin methylesterase (PME) and β-1, 3-glucanase increased, whereas those of covalently wall-bound peroxidase (CPO) and polyphenol oxidase (PPO) decreased. This reduction, along with a decrease in callose, cellulose, and phenolic acid content, likely accelerated cell growth by reducing cell wall crosslinking and stiffness. The content of reactive oxygen/nitrogen species i.e., hydrogen peroxide (H₂O₂), hydroxyl radical (^.OH), and nitric oxide (NO) radicals significantly decreased in response to clinorotation compared with 1g-grown cells. Hierarchical cluster analysis revealed a strong negative correlation between NO and catalase (CAT) activity. The observed decrease in these oxidants can be attributed, at least in part, to the increased content of soluble sugars through the oxidative pentose-phosphate pathway or tricarboxylic acid cycle (TCA), and more significantly, to the enhancement of catalase activity and flavonoid content.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"308 ","pages":"Article 154489"},"PeriodicalIF":4.0,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143785445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unravelling the gene regulatory network linking red leaf and red flesh traits in teinturier grape","authors":"Haoran Li ,&nbsp;Yi Zhang ,&nbsp;Wen Zhang ,&nbsp;Chenxu Sun ,&nbsp;Liyuan Huang ,&nbsp;Yang Dong ,&nbsp;Yaxin Yang ,&nbsp;Hui Li ,&nbsp;Huan Zheng ,&nbsp;Jianmin Tao","doi":"10.1016/j.jplph.2025.154488","DOIUrl":"10.1016/j.jplph.2025.154488","url":null,"abstract":"<div><div>Despite the extensive research conducted on grape anthocyanins, previous studies have predominantly focused on grape skin colour changes, with limited research on flesh colour and leaf colour. In this study, we utilised the superior line 'Zhongshan 151' strain (red flesh and red leaves) as a target and identified that the primary driving force for the transition of leaf colour from green to red was the accumulation of anthocyanins. The study identified a candidate gene, <em>VvMYBA6,</em> and determined that the encoded protein is located in the nucleus and possesses transcriptional activation activity. Subsequent experiments revealed that <em>VvMYBA6</em> significantly promoted anthocyanin accumulation in tobacco through its overexpression. Further mechanistic investigations elucidated the interaction of <em>VvMYBA6</em> with the <em>VvMYC1</em> protein, which activates the expression of <em>VvUFGT,</em> thereby promoting anthocyanin accumulation. Furthermore, an interaction between <em>VvMYBA1</em> and <em>VvMYC1</em> was identified in leaves, which is consistent with the mechanism of flesh colour regulation in red-fleshed grapes and affects anthocyanin accumulation by regulating the expression of <em>VvUFGT</em>. The interaction between <em>VvMYBA1</em> and <em>VvMYBA6</em> was further verified by yeast two-hybrid (Y2H) and pull-down experiments. This finding indicates that the interaction between <em>VvMYBA6, VvMYBA1</em> and <em>VvMYC1</em> plays a pivotal role in the regulation of anthocyanin synthesis, which may significantly impact the development of fruit colour in teinturier grapes.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"308 ","pages":"Article 154488"},"PeriodicalIF":4.0,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143725480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}