In vitro induction and selection of mutants obtained through gamma irradiation with improved processing traits in potato (Solanum tuberosum L.).

IF 2.1 4区医学 Q2 BIOLOGY International Journal of Radiation Biology Pub Date : 2024-01-01 Epub Date: 2023-08-14 DOI:10.1080/09553002.2023.2245472

Rupinder Kaur, Sat Pal Sharma, Anu Kalia, Navraj Kaur, Pooja Manchanda

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引用次数: 0

Abstract

Purpose: This manuscript aimed for the generation of γ-irradiation derived mutants of potato genotype PAU/RR-1501 possessing desirable processing traits.

Materials and methods: Nodal cuttings from virus-free explants were established on basal MS medium and irradiated with different doses (0, 5, 10 and 20 Gy) of γ-irradiation. The 5 and 10 Gy treated plantlets were multiplied and used for micro-tuber induction. Harvested micro-tubers were planted in pots for the selection and evaluation of mutants in M1V2 generation.

Results: Four weeks post-treatment, plantlets (5 Gy) showed enhanced growth as compared to the control while 20 Gy treatment exhibited completely ceased shoot growth. The highest number and weight of mini-tubers per plant was recorded for 10 Gy followed by 5 Gy treatment as compared to control. The γ-irradiation treatments caused changes in the skin color and shape of M1V2 tubers.

Conclusion: Under the 5 Gy treatment 49.9% of clones produced exhibited cream and 8.53% brown skin color. Nine putative mutants were identified in genotype PAU/RR-1501 exhibiting promising processing traits.

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体外诱导和筛选通过伽马辐照获得的突变体，改善马铃薯（Solanum tuberosum L.）的加工性状。

目的：本手稿旨在生成具有理想加工性状的马铃薯基因型 PAU/RR-1501 的γ-辐照衍生突变体：在基础 MS 培养基上建立无病毒外植体的节间插条，并用不同剂量（0、5、10 和 20 Gy）的 γ 辐射照射。经 5 和 10 Gy 处理的小植株被繁殖并用于微管诱导。收获的微管种植在花盆中，用于 M1V2 代突变体的筛选和评估：处理后四周，与对照组相比，5 Gy 处理的小苗生长速度加快，而 20 Gy 处理的小苗则完全停止了生长。与对照相比，10 Gy 处理的每株小管数量和重量最高，其次是 5 Gy 处理。γ-辐照处理导致 M1V2 块茎的表皮颜色和形状发生变化：结论：在 5 Gy 处理下，49.9% 的克隆表现出奶油色，8.53% 的克隆表现出棕色。在基因型 PAU/RR-1501 中发现了 9 个推定突变体，这些突变体具有良好的加工性状。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Radiation Biology 医学-核科学技术

CiteScore

5.00

自引率

11.50%

发文量

142

审稿时长

3 months

期刊介绍： The International Journal of Radiation Biology publishes original papers, reviews, current topic articles, technical notes/reports, and meeting reports on the effects of ionizing, UV and visible radiation, accelerated particles, electromagnetic fields, ultrasound, heat and related modalities. The focus is on the biological effects of such radiations: from radiation chemistry to the spectrum of responses of living organisms and underlying mechanisms, including genetic abnormalities, repair phenomena, cell death, dose modifying agents and tissue responses. Application of basic studies to medical uses of radiation extends the coverage to practical problems such as physical and chemical adjuvants which improve the effectiveness of radiation in cancer therapy. Assessment of the hazards of low doses of radiation is also considered.