{"title":"Production of desmethyl-gregatin A, a possible causative toxin of brown stem rot in adzuki bean, by <i>Phialophora gregata</i> f. sp. <i>adzukicola</i>.","authors":"Moe Aizawa, Hayate Saito, Takuya Mitazaki, Takara Taketani, Keiichi Noguchi, Sho Miyazaki, Hiroshi Kawaide, Masahiro Natsume","doi":"10.1584/jpestics.D22-070","DOIUrl":null,"url":null,"abstract":"<p><p>To elucidate the cause of brown stem rot in the adzuki bean, we re-evaluated the phytotoxins produced in cultures of the causative agent, <i>Phialophora gregata</i> f. sp. <i>adzukicola</i>. The ethyl acetate-soluble acidic fraction of the culture, as well as the neutral fraction, inhibited the growth of alfalfa seedlings. In the neutral fraction, known phytotoxins gregatin A, B, and C or D and penicilliol A were present. Although the phytotoxins in the acidic fraction were unstable, liquid chromatography-mass spectrometry analysis of the partially purified material suggested that one phytotoxin present was the non-methylated gregatin desmethyl-gregatin A (gregatinic acid A).</p>","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":null,"pages":null},"PeriodicalIF":1.5000,"publicationDate":"2023-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/02/59/jps-48-2-D22-070.PMC10288003.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Pesticide Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1584/jpestics.D22-070","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
To elucidate the cause of brown stem rot in the adzuki bean, we re-evaluated the phytotoxins produced in cultures of the causative agent, Phialophora gregata f. sp. adzukicola. The ethyl acetate-soluble acidic fraction of the culture, as well as the neutral fraction, inhibited the growth of alfalfa seedlings. In the neutral fraction, known phytotoxins gregatin A, B, and C or D and penicilliol A were present. Although the phytotoxins in the acidic fraction were unstable, liquid chromatography-mass spectrometry analysis of the partially purified material suggested that one phytotoxin present was the non-methylated gregatin desmethyl-gregatin A (gregatinic acid A).
为了阐明小豆褐色茎腐病的原因,我们重新评估了病原菌phalophora gregata f. sp. adzukicola培养物中产生的植物毒素。乙酸乙酯可溶性酸性部分和中性部分抑制了苜蓿幼苗的生长。在中性部分中,存在已知的植物毒素gregatin A, B和C或D和青霉醇A。虽然酸性部分中的植物毒素不稳定,但液相色谱-质谱分析部分纯化的物质表明,其中一种植物毒素是未甲基化的gregatin去甲基-gregatin A (gregatin acid A)。
期刊介绍:
The Journal of Pesticide Science publishes the results of original research regarding the chemistry and biochemistry of pesticides including bio-based materials. It also covers their metabolism, toxicology, environmental fate and formulation.