Rb analog Whi5 regulates G1 to S transition and cell size but not replicative lifespan in budding yeast

Q2 Medicine Translational Medicine of Aging Pub Date : 2019-01-01 DOI:10.1016/j.tma.2019.10.002

Matthew M. Crane , Mitsuhiro Tsuchiya , Ben W. Blue , Jared D. Almazan , Kenneth L. Chen , Siobhan R. Duffy , Alexandra Golubeva , Annaiz M. Grimm , Alison M. Guard , Shauna A. Hill , Ellen Huynh , Ryan M. Kelly , Michael Kiflezghi , Hyunsung D. Kim , Mitchell Lee , Ting-I. Lee , Jiayi Li , Bao M.G. Nguyen , Riley M. Whalen , Feng Y. Yeh , Matt Kaeberlein

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引用次数: 4

Abstract

An increase in cell size with age is a characteristic feature of replicative aging in budding yeast. Deletion of the gene encoding Whi5 results in shortened duration of G1 and reduced cell size, and has been previously suggested to increase replicative lifespan. Upon careful analysis of multiple independently derived haploid and homozygous diploid whi5Δ mutants, we find no effect on lifespan, but we do confirm the reduction in cell size. We suggest that instead of antagonizing lifespan, the elongated G1 phase of the cell cycle during aging may actually play an important role in allowing aged cells time to repair accumulating DNA damage.

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Rb类似物wh5调节出芽酵母G1向S的转变和细胞大小，但不调节复制寿命

细胞大小随年龄增长而增加是出芽酵母复制老化的一个特征。编码wh5的基因缺失导致G1持续时间缩短，细胞大小减小，并且先前被认为可以延长复制寿命。在仔细分析了多个独立衍生的单倍体和纯合子二倍体whi5Δ突变体后，我们发现对寿命没有影响，但我们确实证实了细胞大小的减少。我们认为，衰老过程中延长的细胞周期G1期实际上可能在允许衰老细胞有时间修复累积的DNA损伤方面发挥重要作用，而不是对抗寿命。

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