Effect of Curcumin-containing Nanofibrous Gelatin-hydroxyapatite Scaffold on Proliferation and Early Osteogenic Differentiation of Dental Pulp Stem Cells.

Q2 Pharmacology, Toxicology and Pharmaceutics Pharmaceutical nanotechnology Pub Date : 2024-01-01 DOI:10.2174/2211738511666230817102159

Solmaz Maleki Dizaj, Yashar Rezaei, Fatemeh Namaki, Simin Sharifi, Elaheh Dalir Abdolahinia

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Abstract

Background: In recent years, the electrospinning method has received attention because of its usage in producing a mimetic nanocomposite scaffold for tissue regeneration. Hydroxyapatite and gelatin are suitable materials for producing scaffolds, and curcumin has the osteogenesis induction effect.

Aims: This study aimed to evaluate the toxicity and early osteogenic differentiation stimulation of nanofibrous gelatin-hydroxyapatite scaffold containing curcumin on dental pulp stem cells (DPSCs).

Objective: The objective of the present investigation was the evaluation of the proliferative effect and primary osteogenic stimulation of DPSCs with a nanofibrous gelatin-hydroxyapatite scaffold containing curcumin. Hydroxyapatite and gelatin were used as suitable and biocompatible materials to make a scaffold suitable for stimulating osteogenesis. Curcumin was added to the scaffold as an osteogenic differentiation- enhancing agent.

Methods: The effect of nano-scaffold on the proliferation of DPSCs was evaluated. The activity of alkaline phosphatase (ALP) as the early osteogenic marker was considered to assess primary osteogenesis stimulation in DPSCs.

Results: The nanofibrous gelatin-hydroxyapatite scaffold containing curcumin significantly increased the proliferation and the ALP activity of DPSCs (P<0.05). The proliferative effect was insignificant in the first 2 days, but the scaffold increased cell proliferation by more than 40% in the fourth and sixth days. The prepared scaffold increased the activity of the ALP of DPSCs by 60% compared with the control after 14 days (p<0.05).

Conclusion: The produced nanofibrous gelatin-hydroxyapatite scaffold containing curcumin can be utilized as a potential candidate in tissue engineering and regeneration of bone and tooth.

Future prospects: The prepared scaffold in the present study could be a beneficial biomaterial for tissue engineering and the regeneration of bone and tooth soon.

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含姜黄素的纳米纤维明胶-羟磷灰石支架对牙髓干细胞增殖和早期成骨分化的影响

背景：近年来，电纺丝方法因其用于生产组织再生的仿生纳米复合支架而受到关注。目的：本研究旨在评估含有姜黄素的纳米纤维明胶-羟基磷灰石支架对牙髓干细胞（DPSCs）的毒性和早期成骨分化刺激作用：本研究的目的是评估含有姜黄素的纳米纤维明胶-羟基磷灰石支架对牙髓干细胞的增殖作用和早期成骨分化刺激。羟基磷灰石和明胶是合适的生物相容性材料，可用于制作刺激成骨的支架。在支架中添加姜黄素作为成骨分化增强剂：方法：评估了纳米支架对 DPSCs 增殖的影响。方法：评估了纳米支架对 DPSCs 增殖的影响，并将碱性磷酸酶（ALP）的活性作为早期成骨标志物，以评估对 DPSCs 初级成骨的刺激作用：结果：含有姜黄素的纳米纤维明胶-羟基磷灰石支架显著提高了DPSCs（PC）的增殖和ALP活性：含有姜黄素的纳米纤维明胶-羟基磷灰石支架可作为组织工程和骨与牙齿再生的潜在候选材料：未来展望：本研究制备的支架可能很快成为组织工程和骨与牙齿再生的一种有益的生物材料。

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来源期刊

Pharmaceutical nanotechnology Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science

CiteScore

4.20

自引率

0.00%

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期刊介绍： Pharmaceutical Nanotechnology publishes original manuscripts, full-length/mini reviews, thematic issues, rapid technical notes and commentaries that provide insights into the synthesis, characterisation and pharmaceutical (or diagnostic) application of materials at the nanoscale. The nanoscale is defined as a size range of below 1 µm. Scientific findings related to micro and macro systems with functionality residing within features defined at the nanoscale are also within the scope of the journal. Manuscripts detailing the synthesis, exhaustive characterisation, biological evaluation, clinical testing and/ or toxicological assessment of nanomaterials are of particular interest to the journal’s readership. Articles should be self contained, centred around a well founded hypothesis and should aim to showcase the pharmaceutical/ diagnostic implications of the nanotechnology approach. Manuscripts should aim, wherever possible, to demonstrate the in vivo impact of any nanotechnological intervention. As reducing a material to the nanoscale is capable of fundamentally altering the material’s properties, the journal’s readership is particularly interested in new characterisation techniques and the advanced properties that originate from this size reduction. Both bottom up and top down approaches to the realisation of nanomaterials lie within the scope of the journal.