Metagenomic analysis of ready biodegradability tests to ascertain the relationship between microbiota and the biodegradability of test chemicals.

IF 1.5 4区 农林科学 Q2 ENTOMOLOGY Journal of Pesticide Science Pub Date : 2023-05-20 DOI:10.1584/jpestics.D22-067
Yoshinari Takano, Saki Takekoshi, Kotaro Takano, Yoshihide Matoba, Makiko Mukumoto, Osamu Shirai
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Abstract

Ready biodegradability tests conducted in accordance with the Organisation for Economic Co-operation and Development guidelines (test 301C or test 301F) are performed using activated sludge (AS) prepared by the Chemicals Evaluation and Research Institute (AS-CERI) or that taken from a sewage treatment plant (AS-STP). It had been reported that AS-CERI had lower activity than AS-STP in biodegrading test chemicals, and that biodegradation was accelerated by increasing the volume of the test medium. However, these phenomena have not been clarified from the perspective of the microbiota. In this study, using metagenomic analysis, we first showed that the microbiota of AS-CERI was biased in its distribution of phyla, less diverse, and had greater lot-to-lot variability than that of AS-STP. Second, after cultivation for a long period of time, the microbiota of AS-STP and AS-CERI became more similar to each other in terms of community structure. Third, determining degraders of test substances when each substance was actively biodegraded was found to be an effective approach. Finally, we clarified experimentally that a large volume of test medium increased the number of species that could degrade test substances in the condition where the initial concentrations of each substance and AS-STP were kept constant.

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对现成的生物降解性试验进行元基因组分析,以确定微生物群与试验化学品的生物降解性之间的关系。
根据经济合作与发展组织准则(试验 301C 或试验 301F)进行的即用生物降解性试验,使用的是化学品评估和研究所(AS-CERI)制备的活性污泥(AS)或从污水处理厂提取的活性污泥(AS-STP)。据报道,AS-CERI 在生物降解测试化学品方面的活性低于 AS-STP,而且生物降解会随着测试介质体积的增加而加快。然而,这些现象尚未从微生物群的角度得到澄清。在本研究中,我们首先利用元基因组分析表明,与 AS-STP 相比,AS-CERI 的微生物区系在门类分布上存在偏差,多样性较低,批次间的变异性较大。其次,经过长期培养后,AS-STP 和 AS-CERI 的微生物群在群落结构上变得更加相似。第三,我们发现在每种物质被积极生物降解时测定其降解者是一种有效的方法。最后,我们通过实验证明,在每种物质和 AS-STP 的初始浓度保持不变的情况下,大量的试验培养基增加了能够降解试验物质的物种数量。
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来源期刊
Journal of Pesticide Science
Journal of Pesticide Science 农林科学-昆虫学
CiteScore
4.30
自引率
4.20%
发文量
28
审稿时长
18-36 weeks
期刊介绍: The Journal of Pesticide Science publishes the results of original research regarding the chemistry and biochemistry of pesticides including bio-based materials. It also covers their metabolism, toxicology, environmental fate and formulation.
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