Combination human umbilical cord perivascular and endothelial colony forming cell therapy for ischemic cardiac injury.

IF 6.4 1区 医学 Q1 CELL & TISSUE ENGINEERING npj Regenerative Medicine Pub Date : 2023-08-25 DOI:10.1038/s41536-023-00321-3
Farwah Iqbal, Alexander Johnston, Brandon Wyse, Razieh Rabani, Poonam Mander, Banafshe Hoseini, Jun Wu, Ren-Ke Li, Andrée Gauthier-Fisher, Peter Szaraz, Clifford Librach
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引用次数: 1

Abstract

Cell-based therapeutics are promising interventions to repair ischemic cardiac tissue. However, no single cell type has yet been found to be both specialized and versatile enough to heal the heart. The synergistic effects of two regenerative cell types including endothelial colony forming cells (ECFC) and first-trimester human umbilical cord perivascular cells (FTM HUCPVC) with endothelial cell and pericyte properties respectively, on angiogenic and regenerative properties were tested in a rat model of myocardial infarction (MI), in vitro tube formation and Matrigel plug assay. The combination of FTM HUCPVCs and ECFCs synergistically reduced fibrosis and cardiomyocyte apoptosis, while promoting favorable cardiac remodeling and contractility. These effects were in part mediated by ANGPT2, PDGF-β, and VEGF-C. PDGF-β signaling-dependent synergistic effects on angiogenesis were also observed in vitro and in vivo. FTM HUCPVCs and ECFCs represent a cell combination therapy for promoting and sustaining vascularization following ischemic cardiac injury.

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脐带血管周围细胞与内皮细胞集落形成细胞联合治疗缺血性心脏损伤。
基于细胞的治疗方法是修复缺血心脏组织的有希望的干预措施。然而,目前还没有发现一种细胞类型既特化又万能,足以治愈心脏。采用大鼠心肌梗死(MI)模型、体外成管实验和Matrigel塞实验,研究了内皮集落形成细胞(ECFC)和孕早期脐带血管周围细胞(FTM HUCPVC)对血管生成和再生特性的协同作用。FTM HUCPVCs和ecfc联合使用可协同减少纤维化和心肌细胞凋亡,同时促进有利的心脏重塑和收缩。这些作用部分是由ANGPT2、PDGF-β和VEGF-C介导的。体外和体内也观察到PDGF-β信号依赖性对血管生成的协同作用。FTM HUCPVCs和ecfc代表了一种促进和维持缺血性心脏损伤后血管化的细胞联合疗法。
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来源期刊
npj Regenerative Medicine
npj Regenerative Medicine Engineering-Biomedical Engineering
CiteScore
10.00
自引率
1.40%
发文量
71
审稿时长
12 weeks
期刊介绍: Regenerative Medicine, an innovative online-only journal, aims to advance research in the field of repairing and regenerating damaged tissues and organs within the human body. As a part of the prestigious Nature Partner Journals series and in partnership with ARMI, this high-quality, open access journal serves as a platform for scientists to explore effective therapies that harness the body's natural regenerative capabilities. With a focus on understanding the fundamental mechanisms of tissue damage and regeneration, npj Regenerative Medicine actively encourages studies that bridge the gap between basic research and clinical tissue repair strategies.
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