Ultraflexible polyvinylidene fluoride film based amperometric enzyme-free sensor for selective detection of uric acid in a trace level.

IF 1.6 4区医学 Q4 BIOPHYSICS Biointerphases Pub Date : 2023-07-01 DOI:10.1116/6.0002792

Abhisek Maikap, Riju Karmakar, Ajit Kumar Meikap, Subhra Samanta

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Abstract

The present invention describes a novel flexible nanosensor for the electrochemical detection of uric acid (UA) present in urine. The synthesized graphite-boron nanocomposite with an average thickness of ∼32 nm was grown up on a flexible polyvinylidene fluoride film with an average thickness of ∼50 μm and it acts as a nonenzymatic sensor for UA. The developed flexible sensor showed a prominent reduction peak in cyclic voltammetry and amperometric response with the presence of different concentrations of aqueous UA solution. In the electrochemical study, the redox peak was generated near ∼-0.42 V with a detection limit of around ∼2.09 μM as the bottom level. The high robustness of the developed sensor originated from the polymeric film base and the rapid response time of ∼0.5 s for detecting UA present in human urine. The interference property of the sensor was confirmed in the presence of bilirubin and creatinine as an eventual reference toward selectivity. The phase and morphology of the sensor surface were extensively observed before and after sensing to comprehend the electrochemical interaction between the sensor and target molecules. The generated quantitative results of the integrated system were verified by testing known and unknown concentrations of UA solutions.

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基于超柔性聚偏氟乙烯薄膜的无酶安培传感器，用于微量尿酸的选择性检测。

本发明描述了一种用于电化学检测尿液中尿酸(UA)的新型柔性纳米传感器。将合成的平均厚度为~ 32 nm的石墨-硼纳米复合材料生长在平均厚度为~ 50 μm的柔性聚偏氟乙烯薄膜上，并作为UA的非酶传感器。所研制的柔性传感器在不同浓度的UA水溶液存在时，在循环伏安和安培响应中表现出明显的还原峰。在电化学研究中，氧化还原峰在~ -0.42 V附近产生，检测限为~ 2.09 μM左右。所开发的传感器的高鲁棒性源于聚合物膜基和检测人体尿液中UA的快速响应时间(~ 0.5 s)。在胆红素和肌酐的存在下，传感器的干扰特性被证实为选择性的最终参考。在传感前后广泛观察传感器表面的物相和形貌，以了解传感器与目标分子之间的电化学相互作用。通过测试已知和未知浓度的UA溶液，验证了集成系统生成的定量结果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Biointerphases 生物-材料科学：生物材料

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期刊介绍： Biointerphases emphasizes quantitative characterization of biomaterials and biological interfaces. As an interdisciplinary journal, a strong foundation of chemistry, physics, biology, engineering, theory, and/or modelling is incorporated into originated articles, reviews, and opinionated essays. In addition to regular submissions, the journal regularly features In Focus sections, targeted on specific topics and edited by experts in the field. Biointerphases is an international journal with excellence in scientific peer-review. Biointerphases is indexed in PubMed and the Science Citation Index (Clarivate Analytics). Accepted papers appear online immediately after proof processing and are uploaded to key citation sources daily. The journal is based on a mixed subscription and open-access model: Typically, authors can publish without any page charges but if the authors wish to publish open access, they can do so for a modest fee. Topics include: bio-surface modification nano-bio interface protein-surface interactions cell-surface interactions in vivo and in vitro systems biofilms / biofouling biosensors / biodiagnostics bio on a chip coatings interface spectroscopy biotribology / biorheology molecular recognition ambient diagnostic methods interface modelling adhesion phenomena.