Changes in Gene Expression After Exposing Arabidopsis thaliana Plants to Nanosecond High Amplitude Electromagnetic Field Pulses

IF 1.8 3区 生物学 Q3 BIOLOGY Bioelectromagnetics Pub Date : 2023-07-06 DOI:10.1002/bem.22475
Alexis Porcher, Nancy Wilmot, Pierre Bonnet, Vincent Procaccio, Alain Vian
{"title":"Changes in Gene Expression After Exposing Arabidopsis thaliana Plants to Nanosecond High Amplitude Electromagnetic Field Pulses","authors":"Alexis Porcher,&nbsp;Nancy Wilmot,&nbsp;Pierre Bonnet,&nbsp;Vincent Procaccio,&nbsp;Alain Vian","doi":"10.1002/bem.22475","DOIUrl":null,"url":null,"abstract":"<p>The biological effects of exposure to electromagnetic fields due to wireless technologies and connected devices are a subject of particular research interest. Ultrashort high-amplitude electromagnetic field pulses delivered to biological samples using immersed electrodes in a dedicated cuvette have widely demonstrated their effectiveness in triggering several cell responses including increased cytosolic calcium concentration and reactive oxygen species (ROS) production. In contrast, the effects of these pulses are poorly documented when electromagnetic pulses are delivered through an antenna. Here we exposed <i>Arabidopsis thaliana</i> plants to 30,000 pulses (237  kV m<sup>−1</sup>, 280 ps rise-time, duration of 500 ps) emitted through a Koshelev antenna and monitored the consequences of electromagnetic fields exposure on the expression levels of several key genes involved in calcium metabolism, signal transduction, ROS, and energy status. We found that this treatment was mostly unable to trigger significant changes in the messenger RNA accumulation of calmodulin, Zinc-Finger protein <i>ZAT12</i>, NADPH oxidase/respiratory burst oxidase homolog (<i>RBOH</i>) isoforms D and F, Catalase (<i>CAT2</i>), glutamate-cystein ligase (<i>GSH1</i>), glutathione synthetase (<i>GSH2</i>), Sucrose non-fermenting-related Kinase 1 (<i>SnRK1</i>) and Target of rapamycin (<i>TOR</i>). In contrast, Ascorbate peroxidases <i>APX-1</i> and APX-6 were significantly induced 3 h after the exposure. These results suggest that this treatment, although quite strong in amplitude, is mostly ineffective in inducing biological effects at the transcriptional level when delivered by an antenna. © 2023 The Authors. Bioelectromagnetics published by Wiley Periodicals LLC on behalf of Bioelectromagnetics Society.</p>","PeriodicalId":8956,"journal":{"name":"Bioelectromagnetics","volume":null,"pages":null},"PeriodicalIF":1.8000,"publicationDate":"2023-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bem.22475","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioelectromagnetics","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/bem.22475","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

The biological effects of exposure to electromagnetic fields due to wireless technologies and connected devices are a subject of particular research interest. Ultrashort high-amplitude electromagnetic field pulses delivered to biological samples using immersed electrodes in a dedicated cuvette have widely demonstrated their effectiveness in triggering several cell responses including increased cytosolic calcium concentration and reactive oxygen species (ROS) production. In contrast, the effects of these pulses are poorly documented when electromagnetic pulses are delivered through an antenna. Here we exposed Arabidopsis thaliana plants to 30,000 pulses (237  kV m−1, 280 ps rise-time, duration of 500 ps) emitted through a Koshelev antenna and monitored the consequences of electromagnetic fields exposure on the expression levels of several key genes involved in calcium metabolism, signal transduction, ROS, and energy status. We found that this treatment was mostly unable to trigger significant changes in the messenger RNA accumulation of calmodulin, Zinc-Finger protein ZAT12, NADPH oxidase/respiratory burst oxidase homolog (RBOH) isoforms D and F, Catalase (CAT2), glutamate-cystein ligase (GSH1), glutathione synthetase (GSH2), Sucrose non-fermenting-related Kinase 1 (SnRK1) and Target of rapamycin (TOR). In contrast, Ascorbate peroxidases APX-1 and APX-6 were significantly induced 3 h after the exposure. These results suggest that this treatment, although quite strong in amplitude, is mostly ineffective in inducing biological effects at the transcriptional level when delivered by an antenna. © 2023 The Authors. Bioelectromagnetics published by Wiley Periodicals LLC on behalf of Bioelectromagnetics Society.

Abstract Image

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
拟南芥暴露于纳秒级高幅电磁场脉冲后的基因表达变化。
接触无线技术和联网设备产生的电磁场对生物的影响是一个特别值得关注的研究课题。使用专用比色皿中的浸入式电极向生物样本输送超短高振幅电磁场脉冲,已广泛证明它们能有效触发多种细胞反应,包括增加细胞膜钙浓度和产生活性氧(ROS)。相比之下,当电磁脉冲通过天线传输时,这些脉冲的效果却鲜有记录。在这里,我们将拟南芥暴露于通过科舍廖夫天线发射的 30,000 个脉冲(237 kV m-1、280 ps 上升时间、500 ps 持续时间)中,并监测了电磁场暴露对涉及钙代谢、信号转导、ROS 和能量状态的几个关键基因表达水平的影响。我们发现,这种处理在很大程度上无法引发钙调蛋白、锌指蛋白 ZAT12、NADPH 氧化酶/呼吸猝灭氧化酶同源物(RBOH)异构体 D 和 F、过氧化氢酶(CAT2)、谷氨酸-半胱氨酸连接酶(GSH1)、谷胱甘肽合成酶(GSH2)、蔗糖不发酵相关激酶 1(SnRK1)和雷帕霉素靶(TOR)的信使 RNA 积累发生显著变化。相比之下,抗坏血酸过氧化物酶 APX-1 和 APX-6 在暴露 3 小时后被显著诱导。这些结果表明,这种处理方法虽然振幅很大,但通过天线传递时在转录水平上对生物效应的诱导大多无效。© 2023 作者。生物电磁学》由 Wiley Periodicals LLC 代表生物电磁学协会出版。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Bioelectromagnetics
Bioelectromagnetics 生物-生物物理
CiteScore
4.60
自引率
0.00%
发文量
44
审稿时长
6-12 weeks
期刊介绍: Bioelectromagnetics is published by Wiley-Liss, Inc., for the Bioelectromagnetics Society and is the official journal of the Bioelectromagnetics Society and the European Bioelectromagnetics Association. It is a peer-reviewed, internationally circulated scientific journal that specializes in reporting original data on biological effects and applications of electromagnetic fields that range in frequency from zero hertz (static fields) to the terahertz undulations and visible light. Both experimental and clinical data are of interest to the journal''s readers as are theoretical papers or reviews that offer novel insights into or criticism of contemporary concepts and theories of field-body interactions. The Bioelectromagnetics Society, which sponsors the journal, also welcomes experimental or clinical papers on the domains of sonic and ultrasonic radiation.
期刊最新文献
Protective effect of radiofrequency exposure against menadione-induced oxidative DNA damage in human neuroblastoma cells: The role of exposure duration and investigation on key molecular targets. Characterising core body temperature response of free-moving C57BL/6 mice to 1.95 GHz whole-body radiofrequency-electromagnetic fields. The influence of eyelashes on electric field distribution and absorbed power density in the cornea under millimeter-wave exposure. Numerical and analytical inspection of magnetic field effects in the radical pair mechanism by a simplified rate equation model. Action potential threshold variability for different electrostimulation models and its potential impact on occupational exposure limit values.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1