Amyloid-β-Induced Transglutaminase 2 Expression and Activities are Modulated by 2-Pentadecyl-2-Oxazoline in Mouse and Human Microglial Cell Lines.

IF 1.8 4区 医学 Q3 CLINICAL NEUROLOGY Current Alzheimer research Pub Date : 2023-01-01 DOI:10.2174/1567205020666230804100831
Andrea Parente, Rosa Giacca, Roberta Arena, Ilenia Rullo, Francesca Guida, Sabatino Maione, Vittorio Gentile
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Abstract

Background: Transglutaminase 2 is an ubiquitously multifunctional enzyme and the most widely studied of the transglutaminase family. Consistent with its role in promoting post-translational modifications of proteins, Transglutaminase 2 is involved in many physiological processes such as apoptosis, signal transduction, and cellular adhesion. Several findings indicate that Transglutaminase 2 plays a role in the pathological processes of various inflammation-related diseases, including neurodegenerative diseases.

Objective: We tested the potential modulatory effects on amyloid-β-induced Transglutaminase 2 expression and activities of 2-pentadecyl-2-oxazoline, a plant-derived agent, which has shown effectiveness against chronic pain and associated neuropsychiatric disorders, both in mouse and human microglial cell lines.

Methods: We used biochemistry, molecular and cell biology techniques to evaluate the potential modulatory effects on amyloid-β-induced Transglutaminase 2 expression and activities of 2- pentadecyl-2-oxazoline in mouse and human microglial cell lines.

Results: 2-pentadecyl-2-oxazoline was able to modulate amyloid-β-induced Transglutaminase 2 expression and activities in mouse and human microglial cell lines.

Conclusion: Transglutaminase 2 confirms its role as a neuroinflammation marker, the inhibition of which could be a potential preventive and therapeutic approach, while 2-pentadecyl-2-oxazoline is a potent modulator of the amyloid-β-induced Transglutaminase 2 expression and activities in mouse and human microglial cell lines.

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2- pentadecyl -2- oxazoline对小鼠和人小胶质细胞中淀粉样蛋白β诱导的转谷氨酰胺酶2的表达和活性的调节
背景:转谷氨酰胺酶2是一种普遍存在的多功能酶,也是转谷氨酰胺酶家族中研究最广泛的酶。除了促进蛋白质翻译后修饰的作用外,转谷氨酰胺酶2还参与许多生理过程,如细胞凋亡、信号转导和细胞粘附。一些研究结果表明,转谷氨酰胺酶2在多种炎症相关疾病的病理过程中发挥作用,包括神经退行性疾病。目的:我们测试了2-pentadecyl-2-oxazoline对淀粉样蛋白β诱导的转谷氨酰胺酶2表达和活性的潜在调节作用。2-pentadecyl-2-oxazoline是一种植物源性药物,在小鼠和人类小胶质细胞系中显示出对慢性疼痛和相关神经精神疾病的有效性。方法:采用生物化学、分子生物学和细胞生物学等技术,研究2- pentadecyl-2-恶唑啉对淀粉样蛋白β诱导的转谷氨酰胺酶2的表达和活性的潜在调节作用。结果:2-十六烷基-2-恶唑啉能够调节淀粉样蛋白β诱导的小鼠和人小胶质细胞系转谷氨酰胺酶2的表达和活性。结论:谷氨酰胺转肽类酶2证实了其作为神经炎症标志物的作用,抑制其可能是一种潜在的预防和治疗方法,而2-pentadecyl-2-oxazoline是淀粉样蛋白β诱导的小鼠和人小胶质细胞系中谷氨酰胺转肽类酶2表达和活性的有效调节剂。
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来源期刊
Current Alzheimer research
Current Alzheimer research 医学-神经科学
CiteScore
4.00
自引率
4.80%
发文量
64
审稿时长
4-8 weeks
期刊介绍: Current Alzheimer Research publishes peer-reviewed frontier review, research, drug clinical trial studies and letter articles on all areas of Alzheimer’s disease. This multidisciplinary journal will help in understanding the neurobiology, genetics, pathogenesis, and treatment strategies of Alzheimer’s disease. The journal publishes objective reviews written by experts and leaders actively engaged in research using cellular, molecular, and animal models. The journal also covers original articles on recent research in fast emerging areas of molecular diagnostics, brain imaging, drug development and discovery, and clinical aspects of Alzheimer’s disease. Manuscripts are encouraged that relate to the synergistic mechanism of Alzheimer''s disease with other dementia and neurodegenerative disorders. Book reviews, meeting reports and letters-to-the-editor are also published. The journal is essential reading for researchers, educators and physicians with interest in age-related dementia and Alzheimer’s disease. Current Alzheimer Research provides a comprehensive ''bird''s-eye view'' of the current state of Alzheimer''s research for neuroscientists, clinicians, health science planners, granting, caregivers and families of this devastating disease.
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