NMR assignment and dynamics of the dimeric form of soluble C-terminal domain major ampullate spidroin 2 from Latrodectus hesperus

IF 0.8 4区 生物学 Q4 BIOPHYSICS Biomolecular NMR Assignments Pub Date : 2023-09-05 DOI:10.1007/s12104-023-10150-6
Nur Alia Oktaviani, Ali D. Malay, Mami Goto, Toshio Nagashima, Fumiaki Hayashi, Keiji Numata
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Abstract

Spider dragline silk has attracted great interest due to its outstanding mechanical properties, which exceed those of man-made synthetic materials. Dragline silk, which is composed of at least major ampullate spider silk protein 1 and 2 (MaSp1 and MaSp2), contains a long repetitive domain flanked by N-terminal and C-terminal domains (NTD and CTD). Despite the small size of the CTD, this domain plays a crucial role as a molecular switch that regulates and directs spider silk self-assembly. In this study, we report the 1H, 13C, and 15N chemical shift assignments of the Latrodectus hesperus MaSp2 CTD in dimeric form at pH 7. Our solution NMR data demonstrated that this protein contains five helix regions connected by a flexible linker.

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赤蛾壶腹蛛素2可溶性c端结构域二聚体的核磁共振分配和动力学研究。
蜘蛛丝由于其优异的力学性能,超过了人造合成材料,引起了人们的极大兴趣。拖丝由至少主要的壶腹蜘蛛丝蛋白1和2(MaSp1和MaSp2)组成,包含一个长的重复结构域,两侧为N端和C端结构域(NTD和CTD)。尽管CTD的尺寸很小,但该结构域作为调节和指导蜘蛛丝自组装的分子开关发挥着至关重要的作用。在本研究中,我们报道了在pH 7下二聚体形式的Latrodectus hesperus MaSp2 CTD的1H、13C和15N化学位移分配。我们的溶液NMR数据表明,这种蛋白质包含五个由柔性接头连接的螺旋区域。
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来源期刊
Biomolecular NMR Assignments
Biomolecular NMR Assignments 生物-光谱学
CiteScore
1.70
自引率
11.10%
发文量
59
审稿时长
6-12 weeks
期刊介绍: Biomolecular NMR Assignments provides a forum for publishing sequence-specific resonance assignments for proteins and nucleic acids as Assignment Notes. Chemical shifts for NMR-active nuclei in macromolecules contain detailed information on molecular conformation and properties. Publication of resonance assignments in Biomolecular NMR Assignments ensures that these data are deposited into a public database at BioMagResBank (BMRB; http://www.bmrb.wisc.edu/), where they are available to other researchers. Coverage includes proteins and nucleic acids; Assignment Notes are processed for rapid online publication and are published in biannual online editions in June and December.
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