Assessing learning in mosquito larvae using video-tracking

IF 2.3 2区 农林科学 Q1 ENTOMOLOGY Journal of insect physiology Pub Date : 2023-09-01 DOI:10.1016/j.jinsphys.2023.104535
Martin Dessart, Miguel Piñeirúa, Claudio R. Lazzari, Fernando J. Guerrieri
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Abstract

Mosquito larvae display a stereotyped escape response when they rest attached to the water surface. It consists in detaching from the surface and diving, to return to the surface after a brief time. It has been shown that this response can be evoked several times, by repeatedly presenting a moving shadow. Diving triggered by a potential danger revealed as a simple bioassay for investigating behavioural responses in mosquito larvae, in particular their ability to learn. In the present work, we describe an automated system, based on video-tracking individuals, and extracting quantitative data of their movements. We validated our system, by reinvestigating the habituation response of larvae of Aedes aegypti reared in the laboratory, and providing original data on field-collected larvae of genera Culex and Anopheles. Habituation could be demonstrated to occur in all the species, even though it was not possible to induce dishabituation in Culex and Anopheles mosquitoes. In addition to non-associative learning, we characterised motor activity in the studied species, thanks to the possibility offered by the tracking system to extract multiple variables. The here-described system and algorithms can be easily adapted to multiple experimental situations and variables of interest.

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使用视频跟踪评估蚊子幼虫的学习情况
当蚊子幼虫附着在水面上休息时,它们会表现出刻板的逃跑反应。它包括脱离水面和潜水,在短暂的时间后返回水面。研究表明,这种反应可以通过反复呈现移动的阴影而被多次唤起。潜在危险引发的潜水是一种简单的生物测定方法,用于调查蚊子幼虫的行为反应,特别是它们的学习能力。在目前的工作中,我们描述了一个基于视频跟踪个人并提取他们运动的定量数据的自动化系统。我们通过重新调查实验室饲养的埃及伊蚊幼虫的适应反应,并提供现场收集的库蚊属和按蚊属幼虫的原始数据,验证了我们的系统。可以证明,所有物种都会出现习性化,尽管不可能在库蚊和按蚊中诱导习性化。除了非联想学习之外,由于跟踪系统提供了提取多个变量的可能性,我们还对所研究物种的运动活动进行了表征。这里描述的系统和算法可以很容易地适应多种实验情况和感兴趣的变量。
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来源期刊
Journal of insect physiology
Journal of insect physiology 生物-昆虫学
CiteScore
4.50
自引率
4.50%
发文量
77
审稿时长
57 days
期刊介绍: All aspects of insect physiology are published in this journal which will also accept papers on the physiology of other arthropods, if the referees consider the work to be of general interest. The coverage includes endocrinology (in relation to moulting, reproduction and metabolism), pheromones, neurobiology (cellular, integrative and developmental), physiological pharmacology, nutrition (food selection, digestion and absorption), homeostasis, excretion, reproduction and behaviour. Papers covering functional genomics and molecular approaches to physiological problems will also be included. Communications on structure and applied entomology can be published if the subject matter has an explicit bearing on the physiology of arthropods. Review articles and novel method papers are also welcomed.
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