Identification and Measurement of Biomarkers at Single Microorganism Level for In Situ Monitoring Deep Ultraviolet Disinfection Process

IF 3.7 4区 生物学 Q1 BIOCHEMICAL RESEARCH METHODS IEEE Transactions on NanoBioscience Pub Date : 2023-09-07 DOI:10.1109/TNB.2023.3312754
Yuxuan Xue;Ye Ma;Zhiyong Sun;Xinyu Liu;Mukun Zhang;Jiawei Zhang;Ning Xi
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Abstract

Since the COVID-19 disease has been further aggravated, the prevention of pathogen transmission becomes a vital issue to restrain casualties. Recent research outcomes have shown the possibilities of the viruses existing on inanimate surfaces up to few days, which carry the risk of touch propagation of the disease. Deep ultraviolet germicide irradiation (UVGI) with the wavelength of 255–280nm has been verified to efficiently disinfect various types of bacteria and virus, which could prevent the aggravation of pandemic spread. Even though considerable experiments and approaches have been applied to evaluate the disinfection effects, there are only few reports about how the individual bio-organism behaves after ultraviolet C (UVC) irradiation, especially in the aspect of mechanical changes. Furthermore, since the standard pathway of virus transmission and reproduction requires the host cell to assemble and transport newly generated virus, the dynamic response of infectious cell is always the vital aspect of virology study. In this work, high power LEDs array has been established with 270nm UVC irradiation to evaluate disinfection capability on various types of bio-organism, and incubator embedded atomic force microscopy (AFM) is used to investigate the single bacterium and virus under UVGI. The real-time tracking of the living Vero cells infected with adenovirus has also been presented in this study. The results show that after sufficient UVGI, the outer shell of bacteria and viruses remain intact in structure, however the bio-organisms lost the capability of reproduction and normal metabolism. The experiment results also indicate that once the host cell is infected with adenovirus, the rapid production of newborn virus capsid will gradually destroy the cellular normal metabolism and lose mechanical integrity.
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识别和测量单个微生物水平的生物标志物,用于现场监测深层紫外线消毒过程。
由于 COVID-19 病情进一步恶化,预防病原体传播成为减少人员伤亡的关键问题。最近的研究结果表明,病毒有可能在无生命的物体表面存在长达数天的时间,这就带来了接触传播疾病的风险。波长为 255-280 纳米的深紫外线杀菌照射(UVGI)已被证实能有效消毒各种细菌和病毒,从而防止大流行病传播的加剧。尽管已有大量实验和方法用于评估消毒效果,但关于紫外线 C(UVC)照射后生物个体的表现,尤其是机械变化方面的报告却寥寥无几。此外,由于病毒传播和繁殖的标准途径需要宿主细胞组装和运输新产生的病毒,因此感染细胞的动态反应一直是病毒学研究的重要方面。在这项工作中,利用 270nm 紫外光照射建立了高功率 LED 阵列,以评估对各类生物的消毒能力,并使用培养箱嵌入式原子力显微镜(AFM)来研究紫外光照射下的单个细菌和病毒。本研究还对感染腺病毒的活体 Vero 细胞进行了实时跟踪。结果表明,经过充分紫外光照射后,细菌和病毒的外壳结构保持完好,但生物体失去了繁殖和正常新陈代谢的能力。实验结果还表明,宿主细胞一旦感染腺病毒,新生病毒壳的快速生成会逐渐破坏细胞的正常新陈代谢,并失去机械完整性。
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来源期刊
IEEE Transactions on NanoBioscience
IEEE Transactions on NanoBioscience 工程技术-纳米科技
CiteScore
7.00
自引率
5.10%
发文量
197
审稿时长
>12 weeks
期刊介绍: The IEEE Transactions on NanoBioscience reports on original, innovative and interdisciplinary work on all aspects of molecular systems, cellular systems, and tissues (including molecular electronics). Topics covered in the journal focus on a broad spectrum of aspects, both on foundations and on applications. Specifically, methods and techniques, experimental aspects, design and implementation, instrumentation and laboratory equipment, clinical aspects, hardware and software data acquisition and analysis and computer based modelling are covered (based on traditional or high performance computing - parallel computers or computer networks).
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