Anti-adhesive effect of naturally obtained dicalcium phosphate dihydrate nanoparticles in the rat uterine wound model.

IF 0.5 4区医学 Q4 SURGERY Cirugia Y Cirujanos Pub Date : 2023-01-01 DOI:10.24875/CIRU.23000097

Begum Kurt, Kerim E Oksuz, Zeynep D Sahin-Inan, Ceylan Hepokur

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Abstract

Objective: In this study, we aimed to compare the anti-adhesive effects of contractubex and dicalcium phosphate dihydrate (DCPD) particles in rats treated with the uterine horn adhesion model.

Materials and methods: Newly adult, 60 Wistar albino rats were used as experimental animals. The modified rat uterine horn adhesion model was used to induce intra-abdominal adhesion. Tumor necrosis factor (TNF)-α, interleukin (IL)-1, vascular endothelial growth factor (VEGF) and transforming growth factor (TGF)-β1 were studied for biochemical and immunohistochemical examination.

Results: TNF-α decreased in each group, while it decreased more in G2 and G3 than in G1. IL-1β decreased in each group, while it decreased the most in G3. TGF-β1 and VEGF localization was less in the G2 compared to G1, the least TGF-β1 and VEGF immunolocalization was detected in the G3 and G4. For both antibodies, the least localization among all groups belonged to G3. From day 7 to day 21, the highest TGF-β1 immunolocalization was observed in G1, lesser localization in G2 and lowest in G3.

Conclusion: DCPD nanoparticles show promise as a clinical antiadhesive agent and should be further evaluated in experimental animal models and human trials.

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天然获得的二水磷酸二钙纳米颗粒在大鼠子宫损伤模型中的抗粘附作用。

目的:比较二水磷酸二钙(DCPD)颗粒对大鼠子宫角粘连模型的抗粘连作用。材料与方法:以新生成年Wistar白化大鼠60只为实验动物。采用改良大鼠子宫角粘连模型诱导腹腔内粘连。对肿瘤坏死因子(TNF)-α、白细胞介素(IL)-1、血管内皮生长因子(VEGF)和转化生长因子(TGF)-β1进行生化和免疫组化检查。结果:TNF-α在各组均呈下降趋势，G2、G3组下降幅度大于G1组。IL-1β在各组均呈下降趋势，其中G3组下降幅度最大。TGF-β1和VEGF在G2中的定位较G1少，G3和G4中TGF-β1和VEGF的免疫定位最少。这两种抗体在所有组中定位最少的都属于G3。从第7天到第21天，G1的TGF-β1免疫定位最高，G2的定位较低，G3的定位最低。结论:DCPD纳米颗粒作为抗黏附剂具有广阔的临床应用前景，值得进一步开展动物实验和人体试验。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cirugia Y Cirujanos 医学-外科

CiteScore

0.90

自引率

20.00%

发文量

207

审稿时长

6-12 weeks

期刊介绍： Cirugía y Cirujanoses exponente del desarrollo académico, científico, médico, quirúrgico y tecnológico en materia de salud en México y en el ámbito internacional. Es una revista bimestral, open access, revisada por pares, que publica en español y en inglés (traducido sin coste para los autores) artículos científicos originales, casos clínicos, artículos de revisión de interés general y cartas al editor. Los artículos se seleccionan y publican siguiendo un riguroso análisis, de acuerdo con los estándares internacionalmente aceptados. Sus espacios están abiertos a los académicos, así como a todo miembro de la comunidad médica que manifieste interés por utilizar este foro para publicar sus trabajos.